Experimental infection of human volunteers with Haemophilus ducreyi does not confer protection against subsequent challenge

Jaffar A. Al-Tawfiq, Katherine L. Palmer, Cheng Yen Chen, Jennifer C. Haley, Barry Katz, Antoinette F. Hood, Stanley Spinola

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Two groups of human volunteers were inoculated with 2 doses of live Haemophilus ducreyi 35000HP. The reinfection group consisted of 7 subjects who previously had participated in experimental infection with 35000HP to the pustular stage of disease. The control group consisted of 7 naive subjects. Papules developed at 92.8% (95% confidence interval [CI], 66.1%-99.8%) of sites inoculated with live bacteria, in the reinfection group, and at 85.7% (95% CI, 57.2%-98.2%) of sites in the control group. Sixty-nine percent (95% CI, 36.8%-90.9%) of papules evolved into pustules in the reinfection group, compared with 41% (95% CI, 15.2%-72.3%) in the control group. The recovery rates of H. ducreyi from surface cultures and the histopathology of biopsies obtained from both groups were similar. Thus, experimental infection to the pustular stage of disease does not provide protective immunity against subsequent challenge.

Original languageEnglish
Pages (from-to)1283-1287
Number of pages5
JournalJournal of Infectious Diseases
Volume179
Issue number5
DOIs
StatePublished - 1999

Fingerprint

Haemophilus ducreyi
Volunteers
Confidence Intervals
Infection
Control Groups
Immunity
Bacteria
Biopsy

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Immunology

Cite this

Experimental infection of human volunteers with Haemophilus ducreyi does not confer protection against subsequent challenge. / Al-Tawfiq, Jaffar A.; Palmer, Katherine L.; Chen, Cheng Yen; Haley, Jennifer C.; Katz, Barry; Hood, Antoinette F.; Spinola, Stanley.

In: Journal of Infectious Diseases, Vol. 179, No. 5, 1999, p. 1283-1287.

Research output: Contribution to journalArticle

Al-Tawfiq, Jaffar A. ; Palmer, Katherine L. ; Chen, Cheng Yen ; Haley, Jennifer C. ; Katz, Barry ; Hood, Antoinette F. ; Spinola, Stanley. / Experimental infection of human volunteers with Haemophilus ducreyi does not confer protection against subsequent challenge. In: Journal of Infectious Diseases. 1999 ; Vol. 179, No. 5. pp. 1283-1287.
@article{22be5348e1684d00a10ebe1230af7677,
title = "Experimental infection of human volunteers with Haemophilus ducreyi does not confer protection against subsequent challenge",
abstract = "Two groups of human volunteers were inoculated with 2 doses of live Haemophilus ducreyi 35000HP. The reinfection group consisted of 7 subjects who previously had participated in experimental infection with 35000HP to the pustular stage of disease. The control group consisted of 7 naive subjects. Papules developed at 92.8{\%} (95{\%} confidence interval [CI], 66.1{\%}-99.8{\%}) of sites inoculated with live bacteria, in the reinfection group, and at 85.7{\%} (95{\%} CI, 57.2{\%}-98.2{\%}) of sites in the control group. Sixty-nine percent (95{\%} CI, 36.8{\%}-90.9{\%}) of papules evolved into pustules in the reinfection group, compared with 41{\%} (95{\%} CI, 15.2{\%}-72.3{\%}) in the control group. The recovery rates of H. ducreyi from surface cultures and the histopathology of biopsies obtained from both groups were similar. Thus, experimental infection to the pustular stage of disease does not provide protective immunity against subsequent challenge.",
author = "Al-Tawfiq, {Jaffar A.} and Palmer, {Katherine L.} and Chen, {Cheng Yen} and Haley, {Jennifer C.} and Barry Katz and Hood, {Antoinette F.} and Stanley Spinola",
year = "1999",
doi = "10.1086/314732",
language = "English",
volume = "179",
pages = "1283--1287",
journal = "Journal of Infectious Diseases",
issn = "0022-1899",
publisher = "Oxford University Press",
number = "5",

}

TY - JOUR

T1 - Experimental infection of human volunteers with Haemophilus ducreyi does not confer protection against subsequent challenge

AU - Al-Tawfiq, Jaffar A.

AU - Palmer, Katherine L.

AU - Chen, Cheng Yen

AU - Haley, Jennifer C.

AU - Katz, Barry

AU - Hood, Antoinette F.

AU - Spinola, Stanley

PY - 1999

Y1 - 1999

N2 - Two groups of human volunteers were inoculated with 2 doses of live Haemophilus ducreyi 35000HP. The reinfection group consisted of 7 subjects who previously had participated in experimental infection with 35000HP to the pustular stage of disease. The control group consisted of 7 naive subjects. Papules developed at 92.8% (95% confidence interval [CI], 66.1%-99.8%) of sites inoculated with live bacteria, in the reinfection group, and at 85.7% (95% CI, 57.2%-98.2%) of sites in the control group. Sixty-nine percent (95% CI, 36.8%-90.9%) of papules evolved into pustules in the reinfection group, compared with 41% (95% CI, 15.2%-72.3%) in the control group. The recovery rates of H. ducreyi from surface cultures and the histopathology of biopsies obtained from both groups were similar. Thus, experimental infection to the pustular stage of disease does not provide protective immunity against subsequent challenge.

AB - Two groups of human volunteers were inoculated with 2 doses of live Haemophilus ducreyi 35000HP. The reinfection group consisted of 7 subjects who previously had participated in experimental infection with 35000HP to the pustular stage of disease. The control group consisted of 7 naive subjects. Papules developed at 92.8% (95% confidence interval [CI], 66.1%-99.8%) of sites inoculated with live bacteria, in the reinfection group, and at 85.7% (95% CI, 57.2%-98.2%) of sites in the control group. Sixty-nine percent (95% CI, 36.8%-90.9%) of papules evolved into pustules in the reinfection group, compared with 41% (95% CI, 15.2%-72.3%) in the control group. The recovery rates of H. ducreyi from surface cultures and the histopathology of biopsies obtained from both groups were similar. Thus, experimental infection to the pustular stage of disease does not provide protective immunity against subsequent challenge.

UR - http://www.scopus.com/inward/record.url?scp=0032969994&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032969994&partnerID=8YFLogxK

U2 - 10.1086/314732

DO - 10.1086/314732

M3 - Article

VL - 179

SP - 1283

EP - 1287

JO - Journal of Infectious Diseases

JF - Journal of Infectious Diseases

SN - 0022-1899

IS - 5

ER -