Experimental infection with Haemophilus ducreyi in persons who are infected with HIV does not cause local or augment systemic viral replication

Diane Janowicz, Klara Tenner-Racz, Paul Racz, Tricia L. Humphreys, Carol Schnizlein-Bick, Kate R. Fortney, Beth Zwickl, Barry Katz, James J. Campbell, David D. Ho, Stanley Spinola

Research output: Contribution to journalArticle

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Abstract

We infected 11 HIV-seropositive volunteers whose CD4+ cell counts were >350 cells/μL (7 of whom were receiving antiretrovirals) with Haemophilus ducreyi. The papule and pustule formation rates were similar to those observed in HIV-seronegative historical control subjects. No subject experienced a sustained change in CD4+ cell count or HIV RNA level. The cellular infiltrate in biopsy samples obtained from the HIV-seropositive and HIV-seronegative subjects did not differ with respect to the percentage of leukocytes, neutrophils, macrophages, or T cells. The CD4+:CD8 + cell ratio in biopsy samples from the HIV-seropositive subjects was 1: 3, the inverse of the ratio seen in the HIV-seronegative subjects (P < .0001). Although CD4+ cells proliferated in lesions, in situ hybridization and reverse-transcription polymerase chain reaction for HIV RNA was negative. We conclude that experimental infection in HIV-seropositive persons is clinically similar to infection in HIV-seronegative persons and does not cause local or augment systemic viral replication. Thus, prompt treatment of chancroid may abrogate increases in viral replication associated with natural disease.

Original languageEnglish
Pages (from-to)1443-1451
Number of pages9
JournalJournal of Infectious Diseases
Volume195
Issue number10
DOIs
StatePublished - May 15 2007

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Haemophilus ducreyi
HIV
Infection
CD4 Lymphocyte Count
HIV Infections
Chancroid
RNA
Biopsy
CD4-CD8 Ratio
Reverse Transcription
In Situ Hybridization
Volunteers
Neutrophils
Leukocytes
Macrophages
T-Lymphocytes
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Immunology

Cite this

Experimental infection with Haemophilus ducreyi in persons who are infected with HIV does not cause local or augment systemic viral replication. / Janowicz, Diane; Tenner-Racz, Klara; Racz, Paul; Humphreys, Tricia L.; Schnizlein-Bick, Carol; Fortney, Kate R.; Zwickl, Beth; Katz, Barry; Campbell, James J.; Ho, David D.; Spinola, Stanley.

In: Journal of Infectious Diseases, Vol. 195, No. 10, 15.05.2007, p. 1443-1451.

Research output: Contribution to journalArticle

Janowicz, Diane ; Tenner-Racz, Klara ; Racz, Paul ; Humphreys, Tricia L. ; Schnizlein-Bick, Carol ; Fortney, Kate R. ; Zwickl, Beth ; Katz, Barry ; Campbell, James J. ; Ho, David D. ; Spinola, Stanley. / Experimental infection with Haemophilus ducreyi in persons who are infected with HIV does not cause local or augment systemic viral replication. In: Journal of Infectious Diseases. 2007 ; Vol. 195, No. 10. pp. 1443-1451.
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