Experiments with Osteoblasts Cultured under Hypergravity Conditions

Melissa A. Kacena, Paul Todd, Louis C. Gerstenfeld, William J. Landis

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

To understand further the role of gravity in osteoblast attachment, osteoblasts were subjected to hypergravity conditions in vitro. Scanning electron microscopy of all confluent coverslips from FPA units show that the number of attached osteoblasts was similar among gravitational levels and growth durations (∼-90 cells/microscopic field). Specifically, confluent 1.0G control cultures contained an average of 91±8 cells/field, 3.3G samples had 88±8 cells/field, and 4. 0G cultures averaged 907plusmn;7 cells/field. The sparsely plated cultures assessed by immunohistochemistry also had similar numbers of cells at each time point (1.0G was similar to 3.3 and 4.0G), but cell number changed from one time point to the next as those cells proliferated. Immunohistochemistry of centrifugea samples showed an increase in number (up to 160% increase) and thickness (up to 49% increase) of actin fibers, a decrease in intensity of fibro-nectin fluorescence (18-23% decrease) and an increase in number of vinculin bulbs (202-374% increase in number of vinculin bulbs/area). While hypergravity exposure did not alter the number of attached osteoblasts, it did result in altered actin, fibronectin, and vinculin elements, changing some aspects of osteoblast-substrate adhesion.

Original languageEnglish (US)
Pages (from-to)28-34
Number of pages7
JournalMicrogravity Science and Technology
Volume15
Issue number1
DOIs
StatePublished - Jan 1 2004
Externally publishedYes

ASJC Scopus subject areas

  • Modeling and Simulation
  • Engineering(all)
  • Physics and Astronomy(all)
  • Applied Mathematics

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