Exploring antigen valency and size effects on capture by immuno-surfaces through analysis and experimentation

Yanan Zheng, A. North, R. Danczyk, T. Webster, Harm HogenEsch, A. Rundell

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Experimental exploration and model development is being pursued for analytical determination of the best configuration and composition of immuno-surfaces for capturing target antigen. The effects of antigen valency, size, and concentration on antigen capture is explored through modeling and analysis of experimental data obtained on a BIACORE 3000. The model of the antigen-immuno-surface interaction is based upon a two-compartment model that describes the mass transport-influenced binding of free analytes to the sensor surface. The lumped model considers the multivalent antigen as a whole with a single pair of association and dissociation rates that vary functionally with antigen valency to incorporate cooperative and steric hindrance effects. The lumped model is revised to incorporate antigen size effects; upon antigen binding, an exclusion area is created within the projection of the antigen on the surface. This effect becomes significant for bacteria target antigens on the scale of 0.1 to 10 microns in diameter, and is reflected in our model as a decrease in the effective surface density of free antibodies.

Original languageEnglish (US)
Title of host publication2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages36-40
Number of pages5
ISBN (Electronic)0780374800, 9780780374805
DOIs
StatePublished - 2002
Externally publishedYes
Event2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Madison, United States
Duration: May 2 2002May 4 2002

Other

Other2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology
CountryUnited States
CityMadison
Period5/2/025/4/02

Fingerprint

Antigens
Surface Antigens
Theoretical Models
Bacteria
Antibodies

Keywords

  • BIACORE
  • concentration and surface density effects
  • mathematical models
  • multivalent antigen capture
  • optimizing immunoassays

ASJC Scopus subject areas

  • Biotechnology
  • Microbiology (medical)

Cite this

Zheng, Y., North, A., Danczyk, R., Webster, T., HogenEsch, H., & Rundell, A. (2002). Exploring antigen valency and size effects on capture by immuno-surfaces through analysis and experimentation. In 2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings (pp. 36-40). [1002260] Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/MMB.2002.1002260

Exploring antigen valency and size effects on capture by immuno-surfaces through analysis and experimentation. / Zheng, Yanan; North, A.; Danczyk, R.; Webster, T.; HogenEsch, Harm; Rundell, A.

2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings. Institute of Electrical and Electronics Engineers Inc., 2002. p. 36-40 1002260.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Zheng, Y, North, A, Danczyk, R, Webster, T, HogenEsch, H & Rundell, A 2002, Exploring antigen valency and size effects on capture by immuno-surfaces through analysis and experimentation. in 2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings., 1002260, Institute of Electrical and Electronics Engineers Inc., pp. 36-40, 2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology, Madison, United States, 5/2/02. https://doi.org/10.1109/MMB.2002.1002260
Zheng Y, North A, Danczyk R, Webster T, HogenEsch H, Rundell A. Exploring antigen valency and size effects on capture by immuno-surfaces through analysis and experimentation. In 2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings. Institute of Electrical and Electronics Engineers Inc. 2002. p. 36-40. 1002260 https://doi.org/10.1109/MMB.2002.1002260
Zheng, Yanan ; North, A. ; Danczyk, R. ; Webster, T. ; HogenEsch, Harm ; Rundell, A. / Exploring antigen valency and size effects on capture by immuno-surfaces through analysis and experimentation. 2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings. Institute of Electrical and Electronics Engineers Inc., 2002. pp. 36-40
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