Exploring the use of five-color flow cytometry to examine the effect of acute Tai Chi practice on pro-inflammatory monocyte subtypes

Hongtao Li, Steven Templeton, Roy W. Geib

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Objective: The purpose of this study was to determine whether five-color flow cytometry could be used to examine the effect of acute Tai Chi (TC) practice on pro-inflammatory monocyte subtypes within the total monocyte population in peripheral blood. Design: A pilot pre- post-intervention design was our experimental model: pre-intervention measurements acted as the control for the post-intervention measurements. The research was conducted at Landsbaum Center for Health Education in a Midwestern community. Healthy subjects were asked to perform their style of TC for one hour. Methods: Four milliliter of peripheral blood was collected in the BD Vacutainer CPT tubes immediately prior and after one hour of TC practice. Peripheral blood mononuclear cells (PBMCs) were isolated following the manufacturer's protocol. PBMCs were then assessed for CD14, CD16, TLR2, TLR4, and HLA-DR expressions using five-color flow cytometry measurement. FlowJo and Graphpad Prism software were used to analyze the results. Results: The proportion of pro-inflammatory CD14+CD16+ monocytes decreased immediately after one hour of TC practice (p<0.01). Conclusion: The results indicate that the five-color flow cytometric process was sufficiently sensitive to be used to explore changes in peripheral blood pro-inflammatory monocytes following one hour of TC practice.

Original languageEnglish (US)
Title of host publication50th Annual Rocky Mountain Bioengineering Symposium and 50th International ISA Biomedical Sciences Instrumentation Symposium 2013
Pages210-216
Number of pages7
StatePublished - Sep 9 2013
Event50th Annual Rocky Mountain Bioengineering Symposium and 50th International ISA Biomedical Sciences Instrumentation Symposium 2013 - Colorado Springs, CO, United States
Duration: Apr 5 2013Apr 7 2013

Publication series

Name50th Annual Rocky Mountain Bioengineering Symposium and 50th International ISA Biomedical Sciences Instrumentation Symposium 2013
Volume493

Other

Other50th Annual Rocky Mountain Bioengineering Symposium and 50th International ISA Biomedical Sciences Instrumentation Symposium 2013
CountryUnited States
CityColorado Springs, CO
Period4/5/134/7/13

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Keywords

  • Anti-inflammatory
  • Flow cytometry
  • Inflammation
  • Monocytes
  • Peripheral blood mononuclear cells
  • TC

ASJC Scopus subject areas

  • Bioengineering
  • Biomedical Engineering

Cite this

Li, H., Templeton, S., & Geib, R. W. (2013). Exploring the use of five-color flow cytometry to examine the effect of acute Tai Chi practice on pro-inflammatory monocyte subtypes. In 50th Annual Rocky Mountain Bioengineering Symposium and 50th International ISA Biomedical Sciences Instrumentation Symposium 2013 (pp. 210-216). (50th Annual Rocky Mountain Bioengineering Symposium and 50th International ISA Biomedical Sciences Instrumentation Symposium 2013; Vol. 493).