Expression and localization of p80 interleukin-1 receptor protein in the rat spinal cord

Xiao Fei Wang, Lan Yin, Jian Guo Hu, Li Dong Huang, Pan Pan Yu, Xiao Yan Jiang, Xiao-Ming Xu, Pei Hua Lu

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The biological effects of interleukin (IL)-1 are mediated by two distinct receptors, the p80 or type I (IL-1RI) and p68 or type II (IL-1RII) receptors. Because IL-1RII has a short, 29-amino acid cytoplasmic domain which may not be sufficient for signaling, there is considerable evidence indicating that IL-1 may signal exclusively through the IL-1RI receptor. Here, we report the expression, distribution, and cellular localization of the IL-1RI protein in the adult rat spinal cord in vivo and embryonic spinal cord in vitro. We found that IL-1RI was expressed in both the gray and white matter throughout the entire length of the spinal cord and was localized in neurons of the anterior horn, astrocytes, oligodendrocytes, and central canal ependymal cells. Interestingly, resting microglia were negative for IL-1RI. In primary cultures obtained from the embryonic day (E) 15 rats, IL-1RI was expressed in neurons, astrocytes, and oligodendrocytes as well as microglia. These data provide both in vivo and in vitro evidence that neurons and glial cells express the IL-1RI proteins. The differential expression of IL-1RI in the developing, but not mature, microglia may indicate the difference of these cells in response to IL-1 stimuli during maturation. The distribution and cellular localization of IL-1RI proteins in the spinal cord provide a molecular basis for understanding the reciprocal interaction between the immune and the central nervous systems.

Original languageEnglish (US)
Pages (from-to)45-53
Number of pages9
JournalJournal of Molecular Neuroscience
Volume29
Issue number1
DOIs
StatePublished - May 2006
Externally publishedYes

Fingerprint

Interleukin-1 Receptors
Interleukins
Rats
Spinal Cord
Interleukin-1
Microglia
Interleukin Receptors
Proteins
Oligodendroglia
Neurons
Astrocytes
Anterior Horn Cells
Interleukin-15
Neuroglia
Neurology
Canals
Central Nervous System
Amino Acids

Keywords

  • Expression
  • Immunohistochemistry
  • Spinal cord
  • Type I interleukin-1 receptor

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry
  • Genetics

Cite this

Wang, X. F., Yin, L., Hu, J. G., Huang, L. D., Yu, P. P., Jiang, X. Y., ... Lu, P. H. (2006). Expression and localization of p80 interleukin-1 receptor protein in the rat spinal cord. Journal of Molecular Neuroscience, 29(1), 45-53. https://doi.org/10.1385/JMN:29:1:45

Expression and localization of p80 interleukin-1 receptor protein in the rat spinal cord. / Wang, Xiao Fei; Yin, Lan; Hu, Jian Guo; Huang, Li Dong; Yu, Pan Pan; Jiang, Xiao Yan; Xu, Xiao-Ming; Lu, Pei Hua.

In: Journal of Molecular Neuroscience, Vol. 29, No. 1, 05.2006, p. 45-53.

Research output: Contribution to journalArticle

Wang, Xiao Fei ; Yin, Lan ; Hu, Jian Guo ; Huang, Li Dong ; Yu, Pan Pan ; Jiang, Xiao Yan ; Xu, Xiao-Ming ; Lu, Pei Hua. / Expression and localization of p80 interleukin-1 receptor protein in the rat spinal cord. In: Journal of Molecular Neuroscience. 2006 ; Vol. 29, No. 1. pp. 45-53.
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