Expression and regulation of versican in neural precursor cells and their lineages

Wen Li Gu, Sai Li Fu, Yan Xia Wang, Ying Li, Xiao Fei Wang, Xiao-Ming Xu, Pei Hua Lu

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Aim: To have a better understanding of the expression and regulation of versican isoforms in neural precursor cells (NPC) and oligodendrogliogenesis. Methods: By immunocytochemistry, RT-PCR, and real-time PCR, we examined the temporal expression of versican in NPC isolated from embryonic d 16 rats as well as in oligodendrocyte (OL) lineage cells induced to differentiate from NPC, which mimicked the oligodendrogliogenesis in vivo. Results: We found that versican was constitutively expressed in NPC and their lineage cells, including neurons, astrocytes, and OL. In addition, 2 versican isoforms, V1/V0 and V2, were found to express at low levels in NPC, but at significantly higher levels in OL lineage cells. The peak expression of versican V2 was found at the oligo-dendrocyte precursor cell stage. Furthermore, the treatment of 2 pro-inflammatory cytokines, TNF-α and IFN-γ, enhanced the transcription of versican V2 in NPC in a dose-dependent manner, but showed no effect on V1/V0 expression. Conclusion: Taken together, our results demonstrate that versican, particularly the inhibitory V2 isoform, is increasingly expressed in OL lineage cells induced to differentiate from NPC. An increase in versican V2 expression after cytokine stimulation implies the interplay between the injury-induced upregulation of inflammatory cytokines and chondroitin sulfate proteoglycan-mediated inhibition of axonal regeneration after central nervous system injury.

Original languageEnglish (US)
Pages (from-to)1519-1530
Number of pages12
JournalActa Pharmacologica Sinica
Volume28
Issue number10
DOIs
StatePublished - Oct 2007
Externally publishedYes

Fingerprint

Versicans
Cell Lineage
Oligodendroglia
Protein Isoforms
Cytokines
Chondroitin Sulfate Proteoglycans
Neurology
Transcription
Nervous System Trauma
Neurons
Rats
Astrocytes
Real-Time Polymerase Chain Reaction
Regeneration

Keywords

  • Chondroitin sulfate proteoglycans
  • Neural precursor cells
  • Oligodendrocyte precursor cells
  • Oligodendrocytes
  • Pro-inflammatory cytokines
  • Versican

ASJC Scopus subject areas

  • Chemistry(all)
  • Pharmacology

Cite this

Expression and regulation of versican in neural precursor cells and their lineages. / Gu, Wen Li; Fu, Sai Li; Wang, Yan Xia; Li, Ying; Wang, Xiao Fei; Xu, Xiao-Ming; Lu, Pei Hua.

In: Acta Pharmacologica Sinica, Vol. 28, No. 10, 10.2007, p. 1519-1530.

Research output: Contribution to journalArticle

Gu, Wen Li ; Fu, Sai Li ; Wang, Yan Xia ; Li, Ying ; Wang, Xiao Fei ; Xu, Xiao-Ming ; Lu, Pei Hua. / Expression and regulation of versican in neural precursor cells and their lineages. In: Acta Pharmacologica Sinica. 2007 ; Vol. 28, No. 10. pp. 1519-1530.
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