Expression Genomics of Cervical Cancer

Molecular Classification and Prediction of Radiotherapy Response by DNA Microarray

Yick Fu Wong, Zachariah E. Selvanayagam, Nien Wei, Joseph Porter, Ragini Vittal, Rong Hu, Yong Lin, Jason Liao, Joe Weichung Shih, Tak Hong Cheung, Keith Wing Kit Lo, So Fan Yim, Shing Kai Yip, Danny Tse Ngong, Nelson Siu, Loucia Kit Ying Chan, Chun Sing Chan, Tony Kong, Elena Kutlina, Randall D. McKinnon & 3 others David T. Denhardt, Khew Voon Chin, Tony Kwok Hung Chung

Research output: Contribution to journalArticle

86 Citations (Scopus)

Abstract

Purpose: The incidence and mortality rates of cervical cancer are declining in the United States; however, worldwide, cervical cancer is still one of the leading causes of death in women, second only to breast cancer. This disparity is at least partially explained by the absence of or comparatively ineffective screening programs in the developing world. Recent advances in expression genomics have enabled the use of DNA microarray to profile gene expression of various cancers. These expression profiles may be suitable for molecular classification and prediction of disease outcome and treatment response. We envision that expression genomics applied in cervical cancer may provide a more rational approach to the classification and treatment of the disease. Experimental Design: In this report, we examined the expression profiles of cervical cancer compared with normal cervical tissues in DNA microarrays that contained approximately 11,000 features that correspond to either human transcripts with known function or anonymous expressed sequence tags. Results: Our results showed that normal cervical tissues were completely segregated from the cancer samples using about 40 genes whose expressions were significantly different between these specimens. In addition, clinical stage IB and stage IIB tumors could also be classified based on their signature expression patterns. Most importantly, some of the tumor samples were further stratified into two major groups based on their response to radiotherapy, and we were able to predict the response of these patients to radiotherapy from their expression profiles. Conclusions: Gene expression profiling by DNA microarray may be used for further molecular classification of disease stages and prediction of treatment response in cervical cancer.

Original languageEnglish (US)
Pages (from-to)5486-5492
Number of pages7
JournalClinical Cancer Research
Volume9
Issue number15
StatePublished - Nov 15 2003
Externally publishedYes

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Genomics
Oligonucleotide Array Sequence Analysis
Uterine Cervical Neoplasms
Radiotherapy
Neoplasms
Expressed Sequence Tags
Gene Expression Profiling
Transcriptome
Cause of Death
Research Design
Breast Neoplasms
Gene Expression
Mortality
Incidence
Therapeutics

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Wong, Y. F., Selvanayagam, Z. E., Wei, N., Porter, J., Vittal, R., Hu, R., ... Chung, T. K. H. (2003). Expression Genomics of Cervical Cancer: Molecular Classification and Prediction of Radiotherapy Response by DNA Microarray. Clinical Cancer Research, 9(15), 5486-5492.

Expression Genomics of Cervical Cancer : Molecular Classification and Prediction of Radiotherapy Response by DNA Microarray. / Wong, Yick Fu; Selvanayagam, Zachariah E.; Wei, Nien; Porter, Joseph; Vittal, Ragini; Hu, Rong; Lin, Yong; Liao, Jason; Shih, Joe Weichung; Cheung, Tak Hong; Lo, Keith Wing Kit; Yim, So Fan; Yip, Shing Kai; Ngong, Danny Tse; Siu, Nelson; Chan, Loucia Kit Ying; Chan, Chun Sing; Kong, Tony; Kutlina, Elena; McKinnon, Randall D.; Denhardt, David T.; Chin, Khew Voon; Chung, Tony Kwok Hung.

In: Clinical Cancer Research, Vol. 9, No. 15, 15.11.2003, p. 5486-5492.

Research output: Contribution to journalArticle

Wong, YF, Selvanayagam, ZE, Wei, N, Porter, J, Vittal, R, Hu, R, Lin, Y, Liao, J, Shih, JW, Cheung, TH, Lo, KWK, Yim, SF, Yip, SK, Ngong, DT, Siu, N, Chan, LKY, Chan, CS, Kong, T, Kutlina, E, McKinnon, RD, Denhardt, DT, Chin, KV & Chung, TKH 2003, 'Expression Genomics of Cervical Cancer: Molecular Classification and Prediction of Radiotherapy Response by DNA Microarray', Clinical Cancer Research, vol. 9, no. 15, pp. 5486-5492.
Wong, Yick Fu ; Selvanayagam, Zachariah E. ; Wei, Nien ; Porter, Joseph ; Vittal, Ragini ; Hu, Rong ; Lin, Yong ; Liao, Jason ; Shih, Joe Weichung ; Cheung, Tak Hong ; Lo, Keith Wing Kit ; Yim, So Fan ; Yip, Shing Kai ; Ngong, Danny Tse ; Siu, Nelson ; Chan, Loucia Kit Ying ; Chan, Chun Sing ; Kong, Tony ; Kutlina, Elena ; McKinnon, Randall D. ; Denhardt, David T. ; Chin, Khew Voon ; Chung, Tony Kwok Hung. / Expression Genomics of Cervical Cancer : Molecular Classification and Prediction of Radiotherapy Response by DNA Microarray. In: Clinical Cancer Research. 2003 ; Vol. 9, No. 15. pp. 5486-5492.
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AU - Porter, Joseph

AU - Vittal, Ragini

AU - Hu, Rong

AU - Lin, Yong

AU - Liao, Jason

AU - Shih, Joe Weichung

AU - Cheung, Tak Hong

AU - Lo, Keith Wing Kit

AU - Yim, So Fan

AU - Yip, Shing Kai

AU - Ngong, Danny Tse

AU - Siu, Nelson

AU - Chan, Loucia Kit Ying

AU - Chan, Chun Sing

AU - Kong, Tony

AU - Kutlina, Elena

AU - McKinnon, Randall D.

AU - Denhardt, David T.

AU - Chin, Khew Voon

AU - Chung, Tony Kwok Hung

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N2 - Purpose: The incidence and mortality rates of cervical cancer are declining in the United States; however, worldwide, cervical cancer is still one of the leading causes of death in women, second only to breast cancer. This disparity is at least partially explained by the absence of or comparatively ineffective screening programs in the developing world. Recent advances in expression genomics have enabled the use of DNA microarray to profile gene expression of various cancers. These expression profiles may be suitable for molecular classification and prediction of disease outcome and treatment response. We envision that expression genomics applied in cervical cancer may provide a more rational approach to the classification and treatment of the disease. Experimental Design: In this report, we examined the expression profiles of cervical cancer compared with normal cervical tissues in DNA microarrays that contained approximately 11,000 features that correspond to either human transcripts with known function or anonymous expressed sequence tags. Results: Our results showed that normal cervical tissues were completely segregated from the cancer samples using about 40 genes whose expressions were significantly different between these specimens. In addition, clinical stage IB and stage IIB tumors could also be classified based on their signature expression patterns. Most importantly, some of the tumor samples were further stratified into two major groups based on their response to radiotherapy, and we were able to predict the response of these patients to radiotherapy from their expression profiles. Conclusions: Gene expression profiling by DNA microarray may be used for further molecular classification of disease stages and prediction of treatment response in cervical cancer.

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