Expression of alcohol dehydrogenase in primary monolayer cultures of rat hepatocytes

David W. Crabb, Ting Kai Li

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

With the use of an extensively modified Leibovitz-15 medium, the alcohol dehydrogenase activity of hepatocytes prepared from male rats was successfully maintained in primary culture at the level observed in freshly isolated hepatocytes. Enzyme activity was higher in freshly isolated cells from female rats than from male rats, but it fell to the level characteristic of the male animals after four days in culture. The levels of activity of the cells in culture from both sexes were unaffected by treatment with estrogens or androgens. The results suggest that the sex-determined differences in alcohol dehydrogenase activity in rats do not arise from direct effects of gonadal steroids on the liver.

Original languageEnglish (US)
Pages (from-to)12-17
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume128
Issue number1
DOIs
StatePublished - Apr 16 1985

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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