Expression of cytolethal distending toxin and hemolysin is not required for pustule formation by Haemophilus ducreyi in human volunteers

R. S. Young, K. R. Fortney, V. Gelfanova, Carrie Phillips, Barry Katz, A. F. Hood, J. L. Latimer, Jr Munson R.S., E. J. Hansen, Stanley Spinola

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Abstract

Haemophilus ducreyi makes cytolethal distending toxin (CDT) and hemolysin. In a previous human challenge trial, an isogenic hemolysin-deficient mutant caused pustules with a rate similar to that of its parent. To test whether CDT was required for pustule formation, six human subjects were inoculated with a CDT mutant and parent at multiple sites. The pustule formation rates were similar at both parent and mutant sites. A CDT and hemolysin double mutant was constructed and tested in five additional subjects. The pustule formation rates were similar for the parent and double mutant. These results indicate that neither the expression of CDT, nor that of hemolysin, nor both are required for pustule formation by H. ducreyi in humans.

Original languageEnglish
Pages (from-to)1938-1942
Number of pages5
JournalInfection and Immunity
Volume69
Issue number3
DOIs
StatePublished - 2001

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Haemophilus ducreyi
Hemolysin Proteins
Volunteers
cytolethal distending toxin

ASJC Scopus subject areas

  • Immunology

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Expression of cytolethal distending toxin and hemolysin is not required for pustule formation by Haemophilus ducreyi in human volunteers. / Young, R. S.; Fortney, K. R.; Gelfanova, V.; Phillips, Carrie; Katz, Barry; Hood, A. F.; Latimer, J. L.; Munson R.S., Jr; Hansen, E. J.; Spinola, Stanley.

In: Infection and Immunity, Vol. 69, No. 3, 2001, p. 1938-1942.

Research output: Contribution to journalArticle

Young, R. S. ; Fortney, K. R. ; Gelfanova, V. ; Phillips, Carrie ; Katz, Barry ; Hood, A. F. ; Latimer, J. L. ; Munson R.S., Jr ; Hansen, E. J. ; Spinola, Stanley. / Expression of cytolethal distending toxin and hemolysin is not required for pustule formation by Haemophilus ducreyi in human volunteers. In: Infection and Immunity. 2001 ; Vol. 69, No. 3. pp. 1938-1942.
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