Expression of DR antigens on early human progenitors of neutrophilic and eosinophilic colonies in diffusion chambers (CFUd)

N. Jacobsen, Hal Broxmeyer, R. J. Winchester, M. A S Moore

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Abstract

Incubation of the non-adherent low density cells from normal human bone marrow with an antiserum to DR antigens markedly inhibited their capacity to form granulocytic colonies in fibrin clot diffusion chambers implanted intraperitoneally into irradiated mice. The inhibitory effect of antiserum was abrogated after absorption of antiserum with B cells. Inhibition was obtained both with and without complement treatment. Neutrophilic colony formation exhibited a higher sensitivity to diluted antiserum to DR antigens as compared with eosinophilic colony formation. These results suggest that DR determinants are expressed on the neutrophilic and eosinophilic progenitor cells (CFUd) which give rise to colonies in diffusion chambers.

Original languageEnglish (US)
Pages (from-to)227-233
Number of pages7
JournalScandinavian Journal of Haematology
Volume24
Issue number3
StatePublished - 1980
Externally publishedYes

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Immune Sera
Antigens
Fibrin
B-Lymphocytes
Stem Cells
Cell Count
Bone Marrow

ASJC Scopus subject areas

  • Hematology

Cite this

Expression of DR antigens on early human progenitors of neutrophilic and eosinophilic colonies in diffusion chambers (CFUd). / Jacobsen, N.; Broxmeyer, Hal; Winchester, R. J.; Moore, M. A S.

In: Scandinavian Journal of Haematology, Vol. 24, No. 3, 1980, p. 227-233.

Research output: Contribution to journalArticle

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AB - Incubation of the non-adherent low density cells from normal human bone marrow with an antiserum to DR antigens markedly inhibited their capacity to form granulocytic colonies in fibrin clot diffusion chambers implanted intraperitoneally into irradiated mice. The inhibitory effect of antiserum was abrogated after absorption of antiserum with B cells. Inhibition was obtained both with and without complement treatment. Neutrophilic colony formation exhibited a higher sensitivity to diluted antiserum to DR antigens as compared with eosinophilic colony formation. These results suggest that DR determinants are expressed on the neutrophilic and eosinophilic progenitor cells (CFUd) which give rise to colonies in diffusion chambers.

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