Expression of EMAP II in the developing and adult mouse

Ulrike E. Knies, Susanne Kröger, Matthias Clauss

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Endothelial monocyte-activating polypeptide II (EMAP II) is a chemoattractant for monocytes and granulocytes. EMAP II is translated as a precursor protein, proEMAP II, and is proteolytically cleaved to become the mature, biologically active cytokine. In this study we show that the EMAP II mRNA and the EMAP II precursor protein are constitutively expressed by all cell types analyzed in vitro, whereas the mature cytokine is only present in the supernatant of apoptotic cells. During mouse embryogenesis we found widespread expression of the EMAP II mRNA with transcripts being abundant in areas of tissue remodeling, where a large number of apoptotic cells could be detected by TUNEL staining. In the adult mouse, strong expression of the EMAP II mRNA is restricted to the brain, testis and thymus. Interestingly, prominent signals for EMAP II mRNA are found in local correlation with sites of apoptosis in thymus and testis but not in the brain. We propose that during development, the generation and release of the mature EMAP II may provide a mechanism for the recruitment of phagocytic cells to sites of programmed cell death. In the adult brain, the generation of mature EMAP II may contribute to the recruitment of monocytes and the immunosurveillance of this tissue.

Original languageEnglish (US)
Pages (from-to)141-151
Number of pages11
JournalApoptosis
Volume5
Issue number2
DOIs
StatePublished - 2000
Externally publishedYes

Fingerprint

Thymus
Brain
Messenger RNA
Protein Precursors
Thymus Gland
Testis
Monocytes
Cells
Tissue
Cytokines
small inducible cytokine subfamily E, member 1
Immunologic Monitoring
Chemotactic Factors
In Situ Nick-End Labeling
Cell death
Phagocytes
Granulocytes
Embryonic Development
Cell Death
Cell Count

Keywords

  • Brain
  • EMAP II
  • Immunosurveillance
  • Monocytes
  • tRNA multi-synthetase complex

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Cell Biology

Cite this

Expression of EMAP II in the developing and adult mouse. / Knies, Ulrike E.; Kröger, Susanne; Clauss, Matthias.

In: Apoptosis, Vol. 5, No. 2, 2000, p. 141-151.

Research output: Contribution to journalArticle

Knies, Ulrike E. ; Kröger, Susanne ; Clauss, Matthias. / Expression of EMAP II in the developing and adult mouse. In: Apoptosis. 2000 ; Vol. 5, No. 2. pp. 141-151.
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