Expression of foreign epitopes on recombinant occlusion bodies of baculoviruses

J. H. McLinden, V. A. Ploplis, Elliot Rosen, E. D. Rosen

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Recombinant polyhedrin proteins of the baculovirus Autographa californica nuclear polyhedrosis virus were constructed to serve as immunologic carriers of foreign epitopes. Several recombinants containing an influenza haemagglutinin epitope were obtained. Three of the five recombinants formed occlusion bodies (OBs) and two did not. All of the recombinant polyhedrin proteins reacted with a monoclonal antibody (mAb) specific for an influenza epitope in Western blots. Presentation of the foreign epitope on the surface of recombinant OBs was demonstrated by specific immunoprecipitation of the anti-influenza mAb with recombinant OBs. The recombinant polyhedrin-influenza epitope fusion protein stimulated an influenza-specific immune response in rabbits.

Original languageEnglish (US)
Pages (from-to)231-237
Number of pages7
JournalVaccine
Volume10
Issue number4
StatePublished - 1992
Externally publishedYes

Fingerprint

Baculoviridae
influenza
Human Influenza
epitopes
Epitopes
Recombinant Proteins
recombinant proteins
monoclonal antibodies
Monoclonal Antibodies
Autographa californica multiple nucleopolyhedrovirus
Nucleopolyhedrovirus
Hemagglutinins
hemagglutinins
Immunoprecipitation
Western blotting
Western Blotting
rabbits
immune response
Rabbits
Proteins

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Virology
  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • veterinary(all)

Cite this

McLinden, J. H., Ploplis, V. A., Rosen, E., & Rosen, E. D. (1992). Expression of foreign epitopes on recombinant occlusion bodies of baculoviruses. Vaccine, 10(4), 231-237.

Expression of foreign epitopes on recombinant occlusion bodies of baculoviruses. / McLinden, J. H.; Ploplis, V. A.; Rosen, Elliot; Rosen, E. D.

In: Vaccine, Vol. 10, No. 4, 1992, p. 231-237.

Research output: Contribution to journalArticle

McLinden, JH, Ploplis, VA, Rosen, E & Rosen, ED 1992, 'Expression of foreign epitopes on recombinant occlusion bodies of baculoviruses', Vaccine, vol. 10, no. 4, pp. 231-237.
McLinden, J. H. ; Ploplis, V. A. ; Rosen, Elliot ; Rosen, E. D. / Expression of foreign epitopes on recombinant occlusion bodies of baculoviruses. In: Vaccine. 1992 ; Vol. 10, No. 4. pp. 231-237.
@article{e7fa93851c0a43d488a00ba47113b5cd,
title = "Expression of foreign epitopes on recombinant occlusion bodies of baculoviruses",
abstract = "Recombinant polyhedrin proteins of the baculovirus Autographa californica nuclear polyhedrosis virus were constructed to serve as immunologic carriers of foreign epitopes. Several recombinants containing an influenza haemagglutinin epitope were obtained. Three of the five recombinants formed occlusion bodies (OBs) and two did not. All of the recombinant polyhedrin proteins reacted with a monoclonal antibody (mAb) specific for an influenza epitope in Western blots. Presentation of the foreign epitope on the surface of recombinant OBs was demonstrated by specific immunoprecipitation of the anti-influenza mAb with recombinant OBs. The recombinant polyhedrin-influenza epitope fusion protein stimulated an influenza-specific immune response in rabbits.",
author = "McLinden, {J. H.} and Ploplis, {V. A.} and Elliot Rosen and Rosen, {E. D.}",
year = "1992",
language = "English (US)",
volume = "10",
pages = "231--237",
journal = "Vaccine",
issn = "0264-410X",
publisher = "Elsevier BV",
number = "4",

}

TY - JOUR

T1 - Expression of foreign epitopes on recombinant occlusion bodies of baculoviruses

AU - McLinden, J. H.

AU - Ploplis, V. A.

AU - Rosen, Elliot

AU - Rosen, E. D.

PY - 1992

Y1 - 1992

N2 - Recombinant polyhedrin proteins of the baculovirus Autographa californica nuclear polyhedrosis virus were constructed to serve as immunologic carriers of foreign epitopes. Several recombinants containing an influenza haemagglutinin epitope were obtained. Three of the five recombinants formed occlusion bodies (OBs) and two did not. All of the recombinant polyhedrin proteins reacted with a monoclonal antibody (mAb) specific for an influenza epitope in Western blots. Presentation of the foreign epitope on the surface of recombinant OBs was demonstrated by specific immunoprecipitation of the anti-influenza mAb with recombinant OBs. The recombinant polyhedrin-influenza epitope fusion protein stimulated an influenza-specific immune response in rabbits.

AB - Recombinant polyhedrin proteins of the baculovirus Autographa californica nuclear polyhedrosis virus were constructed to serve as immunologic carriers of foreign epitopes. Several recombinants containing an influenza haemagglutinin epitope were obtained. Three of the five recombinants formed occlusion bodies (OBs) and two did not. All of the recombinant polyhedrin proteins reacted with a monoclonal antibody (mAb) specific for an influenza epitope in Western blots. Presentation of the foreign epitope on the surface of recombinant OBs was demonstrated by specific immunoprecipitation of the anti-influenza mAb with recombinant OBs. The recombinant polyhedrin-influenza epitope fusion protein stimulated an influenza-specific immune response in rabbits.

UR - http://www.scopus.com/inward/record.url?scp=0026549055&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026549055&partnerID=8YFLogxK

M3 - Article

VL - 10

SP - 231

EP - 237

JO - Vaccine

JF - Vaccine

SN - 0264-410X

IS - 4

ER -