Expression of foreign epitopes on recombinant occlusion bodies of baculoviruses

J. H. McLinden, M. J. Fraser, E. D. Rosen

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Recombinant polyhedrin proteins of the baculovirus Autographa californica nuclear polyhedrosis virus were constructed to serve as immunologic carriers of foreign epitopes. Several recombinants containing an influenza haemagglutinin epitope were obtained. Three of the five recombinants formed occlusion bodies (OBs) and two did not. All of the recombinant polyhedrin proteins reacted with a monoclonal antibody (mAb ) specific for an influenza epitope in Western blots. Presentation of the foreign epitope on the surface of recombinant OBs was demonstrated by specific immunoprecipitation of the anti-influenza mAb with recombinant OBs. The recombinant polyhedrin-influenza epitope fusion protein stimulated an influenza-specific immune response in rabbits.

Original languageEnglish (US)
Pages (from-to)231-237
Number of pages7
JournalTopics in Catalysis
Volume10
Issue number4
DOIs
StatePublished - Jan 1 1992

Fingerprint

Baculoviridae
influenza
Human Influenza
epitopes
Epitopes
Recombinant Proteins
recombinant proteins
monoclonal antibodies
Monoclonal Antibodies
Autographa californica multiple nucleopolyhedrovirus
Nucleopolyhedrovirus
Hemagglutinins
hemagglutinins
Immunoprecipitation
Western blotting
Western Blotting
rabbits
immune response
Rabbits
Proteins

Keywords

  • Autographa californica
  • baculovirus
  • epitope
  • influenza
  • occlusion bodies

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Immunology
  • Microbiology
  • Virology
  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • veterinary(all)

Cite this

Expression of foreign epitopes on recombinant occlusion bodies of baculoviruses. / McLinden, J. H.; Fraser, M. J.; Rosen, E. D.

In: Topics in Catalysis, Vol. 10, No. 4, 01.01.1992, p. 231-237.

Research output: Contribution to journalArticle

McLinden, J. H. ; Fraser, M. J. ; Rosen, E. D. / Expression of foreign epitopes on recombinant occlusion bodies of baculoviruses. In: Topics in Catalysis. 1992 ; Vol. 10, No. 4. pp. 231-237.
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