Expression of human papillomavirus type 16 E7 is sufficient to significantly increase expression of angiogenic factors but is not sufficient to induce endothelial cell migration

Joanna Walker, Lucy Clare Smiley, David Ingram, Ann Roman

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Tumor suppressors negatively regulate angiogenesis, an essential step in tumor progression. Together, HPV 16 E6 and E7 proteins, which target p53 and pRb family members, respectively, for degradation, increase the expression of two angiogenic inducers, VEGF and IL-8, in primary foreskin keratinocytes (HFKs). Conditioned media from such cells are sufficient to alter endothelial cell behavior. Here, the individual contribution of E6 and E7 to angiogenesis was investigated. E7 and, to a lesser extent E6, increased expression of VEGF and IL-8. Nevertheless, neither conditioned media from HPV 16 E6 nor E7-expressing HFKs were sufficient to induce migration of endothelial cells. Conditioned media from HFKs expressing the HPV 16 E6 and the E7 mutant E7C24G, which can target p107 and p130 but not pRb for degradation, contained increased levels of VEGF and IL-8. The results suggest that the mechanism of HPV 16 E7-mediated increased levels of VEGF is pRb-independent.

Original languageEnglish
Pages (from-to)283-290
Number of pages8
JournalVirology
Volume410
Issue number2
DOIs
StatePublished - Feb 20 2011

Fingerprint

Human papillomavirus 16
Angiogenesis Inducing Agents
Vascular Endothelial Growth Factor A
Cell Movement
Endothelial Cells
Conditioned Culture Medium
Interleukin-8
Foreskin
Keratinocytes
Neoplasms

Keywords

  • Angiogenesis
  • Human foreskin keratinocytes
  • Human microvascular endothelial cells
  • Human papillomaviruses
  • IL-8
  • P107
  • P130
  • PRb
  • VEGF

ASJC Scopus subject areas

  • Virology

Cite this

Expression of human papillomavirus type 16 E7 is sufficient to significantly increase expression of angiogenic factors but is not sufficient to induce endothelial cell migration. / Walker, Joanna; Smiley, Lucy Clare; Ingram, David; Roman, Ann.

In: Virology, Vol. 410, No. 2, 20.02.2011, p. 283-290.

Research output: Contribution to journalArticle

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