Expression of Ia-like antigen molecules on human granulocytes during early phases of differentiation

R. J. Winchester, G. D. Ross, C. I. Jarowski, C. Y. Wang, J. Halper, Hal Broxmeyer

Research output: Contribution to journalArticle

133 Citations (Scopus)

Abstract

Human B lymphocyte antigens analogous to the murine Ia determinants were found on myeloblasts and promyelocytes but not on more mature granulocytes. This was apparent by fluorescent staining with both human alloantisera and rabbit antisera to the isolated Ia-like proteins. The cells of patients with chronic myelocytic leukemia showed this difference especially clearly. Separation of the myeloblasts and promyelocytes by multistep density gradient fractionation produced a marked enrichment of the positive cells. The remaining cells from higher density fractions were more-mature neutrophils that were essentially negative. In acute myeloid leukemia, in which myeloid cells early in differentiation predominate, the vast majority of cells were strongly positive. Similar results were obtained with normal bone marrow cells. Here also, only the early forms of the myeloid series separated by gradient centrifugation had Ia antigens. Evidence was also obtained for the presence of Ia determinants on cells with the appearance of early erythroid precursors. Support for the presence of the Ia determinants on granulocyte-macrophage committed stem cells was provided by the inhibition of granulocyte colony formation in agar cultures following preincubation of normal bone marrow with antiserum and complement. Cross absorptions with purified preparations of immature cells provided evidence for the close similarity of the antigenic determinants on both myeloblasts and B cells. A 28,000-37,000-dalton bimolecular complex obtained from myeloblast membranes contained the Ia determinants and was similar to that obtained from peripheral blood B cell membranes.

Original languageEnglish (US)
Pages (from-to)4012-4016
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume74
Issue number9
DOIs
StatePublished - 1977
Externally publishedYes

Fingerprint

Granulocyte Precursor Cells
Histocompatibility Antigens Class II
Granulocytes
B-Lymphocytes
Immune Sera
Myeloid Cells
Leukemia, Myelogenous, Chronic, BCR-ABL Positive
Centrifugation
Acute Myeloid Leukemia
Bone Marrow Cells
Agar
Epitopes
Blood Cells
Neutrophils
Stem Cells
Cell Count
Bone Marrow
Macrophages
Cell Membrane
Staining and Labeling

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Expression of Ia-like antigen molecules on human granulocytes during early phases of differentiation. / Winchester, R. J.; Ross, G. D.; Jarowski, C. I.; Wang, C. Y.; Halper, J.; Broxmeyer, Hal.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 74, No. 9, 1977, p. 4012-4016.

Research output: Contribution to journalArticle

@article{88633dae854746e5b8122624e2bcc1a2,
title = "Expression of Ia-like antigen molecules on human granulocytes during early phases of differentiation",
abstract = "Human B lymphocyte antigens analogous to the murine Ia determinants were found on myeloblasts and promyelocytes but not on more mature granulocytes. This was apparent by fluorescent staining with both human alloantisera and rabbit antisera to the isolated Ia-like proteins. The cells of patients with chronic myelocytic leukemia showed this difference especially clearly. Separation of the myeloblasts and promyelocytes by multistep density gradient fractionation produced a marked enrichment of the positive cells. The remaining cells from higher density fractions were more-mature neutrophils that were essentially negative. In acute myeloid leukemia, in which myeloid cells early in differentiation predominate, the vast majority of cells were strongly positive. Similar results were obtained with normal bone marrow cells. Here also, only the early forms of the myeloid series separated by gradient centrifugation had Ia antigens. Evidence was also obtained for the presence of Ia determinants on cells with the appearance of early erythroid precursors. Support for the presence of the Ia determinants on granulocyte-macrophage committed stem cells was provided by the inhibition of granulocyte colony formation in agar cultures following preincubation of normal bone marrow with antiserum and complement. Cross absorptions with purified preparations of immature cells provided evidence for the close similarity of the antigenic determinants on both myeloblasts and B cells. A 28,000-37,000-dalton bimolecular complex obtained from myeloblast membranes contained the Ia determinants and was similar to that obtained from peripheral blood B cell membranes.",
author = "Winchester, {R. J.} and Ross, {G. D.} and Jarowski, {C. I.} and Wang, {C. Y.} and J. Halper and Hal Broxmeyer",
year = "1977",
doi = "10.1073/pnas.74.9.4012",
language = "English (US)",
volume = "74",
pages = "4012--4016",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "9",

}

TY - JOUR

T1 - Expression of Ia-like antigen molecules on human granulocytes during early phases of differentiation

AU - Winchester, R. J.

AU - Ross, G. D.

AU - Jarowski, C. I.

AU - Wang, C. Y.

AU - Halper, J.

AU - Broxmeyer, Hal

PY - 1977

Y1 - 1977

N2 - Human B lymphocyte antigens analogous to the murine Ia determinants were found on myeloblasts and promyelocytes but not on more mature granulocytes. This was apparent by fluorescent staining with both human alloantisera and rabbit antisera to the isolated Ia-like proteins. The cells of patients with chronic myelocytic leukemia showed this difference especially clearly. Separation of the myeloblasts and promyelocytes by multistep density gradient fractionation produced a marked enrichment of the positive cells. The remaining cells from higher density fractions were more-mature neutrophils that were essentially negative. In acute myeloid leukemia, in which myeloid cells early in differentiation predominate, the vast majority of cells were strongly positive. Similar results were obtained with normal bone marrow cells. Here also, only the early forms of the myeloid series separated by gradient centrifugation had Ia antigens. Evidence was also obtained for the presence of Ia determinants on cells with the appearance of early erythroid precursors. Support for the presence of the Ia determinants on granulocyte-macrophage committed stem cells was provided by the inhibition of granulocyte colony formation in agar cultures following preincubation of normal bone marrow with antiserum and complement. Cross absorptions with purified preparations of immature cells provided evidence for the close similarity of the antigenic determinants on both myeloblasts and B cells. A 28,000-37,000-dalton bimolecular complex obtained from myeloblast membranes contained the Ia determinants and was similar to that obtained from peripheral blood B cell membranes.

AB - Human B lymphocyte antigens analogous to the murine Ia determinants were found on myeloblasts and promyelocytes but not on more mature granulocytes. This was apparent by fluorescent staining with both human alloantisera and rabbit antisera to the isolated Ia-like proteins. The cells of patients with chronic myelocytic leukemia showed this difference especially clearly. Separation of the myeloblasts and promyelocytes by multistep density gradient fractionation produced a marked enrichment of the positive cells. The remaining cells from higher density fractions were more-mature neutrophils that were essentially negative. In acute myeloid leukemia, in which myeloid cells early in differentiation predominate, the vast majority of cells were strongly positive. Similar results were obtained with normal bone marrow cells. Here also, only the early forms of the myeloid series separated by gradient centrifugation had Ia antigens. Evidence was also obtained for the presence of Ia determinants on cells with the appearance of early erythroid precursors. Support for the presence of the Ia determinants on granulocyte-macrophage committed stem cells was provided by the inhibition of granulocyte colony formation in agar cultures following preincubation of normal bone marrow with antiserum and complement. Cross absorptions with purified preparations of immature cells provided evidence for the close similarity of the antigenic determinants on both myeloblasts and B cells. A 28,000-37,000-dalton bimolecular complex obtained from myeloblast membranes contained the Ia determinants and was similar to that obtained from peripheral blood B cell membranes.

UR - http://www.scopus.com/inward/record.url?scp=0017749335&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0017749335&partnerID=8YFLogxK

U2 - 10.1073/pnas.74.9.4012

DO - 10.1073/pnas.74.9.4012

M3 - Article

C2 - 71738

AN - SCOPUS:0017749335

VL - 74

SP - 4012

EP - 4016

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 9

ER -