Expression of IGF-I, IGF-I receptor and IGF binding proteins -1, -2, -3, -4 and -5 in human atherectomy specimens

M. B. Grant, T. J. Wargovich, E. A. Ellis, R. Tarnuzzer, S. Caballero, K. Estes, M. Rossing, P. E. Spoerri, C. Pepine

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

The molecular and cellular processes that induce rapid atherosclerotic plaque progression in patients with unstable angina and initiate restenosis following coronary interventional procedures are uncertain. We examined primary (de novo) and restenotic lesions retrieved at the time of directional coronary atherectomy for expression of insulin-like-growth factor-I (IGF-I), IGF-I receptor, and five IGF binding proteins (IGFBPs), IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, and IGFBP-5 in smooth muscle cells (SMCs) using colloidal gold immunocytochemistry. IGF-I, its receptor and binding proteins were not detected in SMCs of normal coronary arteries. IGF-I localized primarily in synthetic smooth muscle cells (sSMCs) in both de novo and restenotic plaques. IGF-I receptor localized on sSMCs and their processes and colocalized with IGF-I. Although morphometric analysis of IGF-I and IGF-I receptor immunoreactivity in sSMCs of de novo and restenotic lesions showed comparable levels of IGF-I (3.2 ± 1.0 and 2.9 ± 0.9, respectively), IGF-I receptor was significantly higher in de novo lesions as compared to restenotic lesions (10.7 ± 2.5 and 4.2 ± 1.3, P <0.05, respectively). IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4 and IGFBP-5 localized in the cytoplasm of sSMCs and in the extracellular matrix. Quantitative reverse transcription polymerase chain reaction (QRT-PCR) performed on de novo atherectomy specimens identified mRNA for IGF-I, IGF-I receptor, IGFBP-1, IGFBP-2, IGFBP-4, IGFBP-5 levels and detected mRNA for IGFBP-3. The expression of IGF-I, IGF-I receptor, and IGFBPs in atherectomy plaques suggests that the development of coronary obstructive lesions may be a result of changes in the IGF system.

Original languageEnglish (US)
Pages (from-to)137-144
Number of pages8
JournalRegulatory Peptides
Volume67
Issue number3
DOIs
StatePublished - Dec 17 1996
Externally publishedYes

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Atherectomy
Insulin-Like Growth Factor Binding Protein 2
Insulin-Like Growth Factor Binding Protein 1
IGF Type 1 Receptor
Insulin-Like Growth Factor I
Smooth Muscle Myocytes
Muscle
Insulin-Like Growth Factor Binding Protein 5
Insulin-Like Growth Factor Binding Protein 4
Insulin-Like Growth Factor Binding Protein 3
Insulin-Like Growth Factor Binding Proteins
Cells
Coronary Atherectomy
Coronary Restenosis
Gold Colloid
Messenger RNA
Polymerase chain reaction
Unstable Angina
Atherosclerotic Plaques
Transcription

Keywords

  • atherectomy
  • IGF binding protein
  • IGF-I receptor
  • immunocytochemistry
  • quantitative RT-PCR
  • smooth muscle cell

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Neuroscience(all)

Cite this

Grant, M. B., Wargovich, T. J., Ellis, E. A., Tarnuzzer, R., Caballero, S., Estes, K., ... Pepine, C. (1996). Expression of IGF-I, IGF-I receptor and IGF binding proteins -1, -2, -3, -4 and -5 in human atherectomy specimens. Regulatory Peptides, 67(3), 137-144. https://doi.org/10.1016/S0167-0115(96)00124-3

Expression of IGF-I, IGF-I receptor and IGF binding proteins -1, -2, -3, -4 and -5 in human atherectomy specimens. / Grant, M. B.; Wargovich, T. J.; Ellis, E. A.; Tarnuzzer, R.; Caballero, S.; Estes, K.; Rossing, M.; Spoerri, P. E.; Pepine, C.

In: Regulatory Peptides, Vol. 67, No. 3, 17.12.1996, p. 137-144.

Research output: Contribution to journalArticle

Grant, MB, Wargovich, TJ, Ellis, EA, Tarnuzzer, R, Caballero, S, Estes, K, Rossing, M, Spoerri, PE & Pepine, C 1996, 'Expression of IGF-I, IGF-I receptor and IGF binding proteins -1, -2, -3, -4 and -5 in human atherectomy specimens', Regulatory Peptides, vol. 67, no. 3, pp. 137-144. https://doi.org/10.1016/S0167-0115(96)00124-3
Grant, M. B. ; Wargovich, T. J. ; Ellis, E. A. ; Tarnuzzer, R. ; Caballero, S. ; Estes, K. ; Rossing, M. ; Spoerri, P. E. ; Pepine, C. / Expression of IGF-I, IGF-I receptor and IGF binding proteins -1, -2, -3, -4 and -5 in human atherectomy specimens. In: Regulatory Peptides. 1996 ; Vol. 67, No. 3. pp. 137-144.
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abstract = "The molecular and cellular processes that induce rapid atherosclerotic plaque progression in patients with unstable angina and initiate restenosis following coronary interventional procedures are uncertain. We examined primary (de novo) and restenotic lesions retrieved at the time of directional coronary atherectomy for expression of insulin-like-growth factor-I (IGF-I), IGF-I receptor, and five IGF binding proteins (IGFBPs), IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, and IGFBP-5 in smooth muscle cells (SMCs) using colloidal gold immunocytochemistry. IGF-I, its receptor and binding proteins were not detected in SMCs of normal coronary arteries. IGF-I localized primarily in synthetic smooth muscle cells (sSMCs) in both de novo and restenotic plaques. IGF-I receptor localized on sSMCs and their processes and colocalized with IGF-I. Although morphometric analysis of IGF-I and IGF-I receptor immunoreactivity in sSMCs of de novo and restenotic lesions showed comparable levels of IGF-I (3.2 ± 1.0 and 2.9 ± 0.9, respectively), IGF-I receptor was significantly higher in de novo lesions as compared to restenotic lesions (10.7 ± 2.5 and 4.2 ± 1.3, P <0.05, respectively). IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4 and IGFBP-5 localized in the cytoplasm of sSMCs and in the extracellular matrix. Quantitative reverse transcription polymerase chain reaction (QRT-PCR) performed on de novo atherectomy specimens identified mRNA for IGF-I, IGF-I receptor, IGFBP-1, IGFBP-2, IGFBP-4, IGFBP-5 levels and detected mRNA for IGFBP-3. The expression of IGF-I, IGF-I receptor, and IGFBPs in atherectomy plaques suggests that the development of coronary obstructive lesions may be a result of changes in the IGF system.",
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AU - Ellis, E. A.

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