Expression of OmpP2A and OmpP2B is not required for pustule formation by Haemophilus ducreyi in human volunteers

Diane Janowicz, Nicole R. Luke, Kate R. Fortney, Barry Katz, Anthony A. Campagnari, Stanley Spinola

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Haemophilus ducreyi express two porin proteins, termed OmpP2A and OmpP2B. To test whether expression of OmpP2A and OmpP2B was necessary for virulence in humans, eight volunteers were experimentally infected with the parent (35000HP) in one arm and a double OmpP2A OmpP2B mutant (35000HP::P2AB) in the other arm. The pustule formation rates were 58.3% (95% CI, 33.2-83.5%) for the parent and 41.7% (95% CI, 19.3-64.0%) for the mutant (P=0.25). Biopsy of 35000HP and 35000HP::P2AB-infected sites yielded similar amounts of bacteria in quantitative culture. These results indicate that expression of OmpP2A and OmpP2B is not necessary to initiate disease or to progress to pustule formation in humans.

Original languageEnglish
Pages (from-to)110-115
Number of pages6
JournalMicrobial Pathogenesis
Volume40
Issue number3
DOIs
StatePublished - Mar 2006

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Haemophilus ducreyi
Volunteers
Porins
Virulence
Bacteria
Biopsy
Proteins

Keywords

  • Haemophilus ducreyi
  • Human challenge model
  • Porin proteins
  • Pustule formation
  • Uirulence

ASJC Scopus subject areas

  • Microbiology
  • Infectious Diseases

Cite this

Expression of OmpP2A and OmpP2B is not required for pustule formation by Haemophilus ducreyi in human volunteers. / Janowicz, Diane; Luke, Nicole R.; Fortney, Kate R.; Katz, Barry; Campagnari, Anthony A.; Spinola, Stanley.

In: Microbial Pathogenesis, Vol. 40, No. 3, 03.2006, p. 110-115.

Research output: Contribution to journalArticle

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