Expression of protein kinase C isozymes in nonpregnant and pregnant human myometrium

William W. Hurd, Victor P. Fomin, Viswanathan Natarajan, Haywood L. Brown, Robert Bigsby, Dawn M. Singh

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

OBJECTIVE: The aim of this study was to compare the distributions of protein kinase C isozymes in human nonpregnant and pregnant myometrial tissues and primary cell cultures. STUDY DESIGN: Myometrial tissues were obtained at hysterectomy from nonpregnant women and at cesarean delivery from women both before and during early labor at term. Western immunoblot analysis was performed on homogenates of myometrial tissues and primary cell cultures with monoclonal antibodies specific for protein kinase C isozymes. Redistribution and translocation of protein kinase C were examined by means of immunocytochemical methods. RESULTS: Nonpregnant myometrial tissues contained protein kinase C isozymes α, γ, δ, μ, ι, and ζ but not β 1, β 2, θ, or ε. Pregnant myometrial tissues both before and during early labor contained the same protein kinase C isozymes and also β 1 andβ 2. The protein kinase C isozyme distribution in primary myometrial cell cultures was identical to that in the myometrial tissues. Protein kinase C redistribution and translocation were demonstrated in these cultured myometrial cells. CONCLUSION: Both human myometrial tissues and primary cell cultures expressed a broad range of protein kinase C isozymes. Protein kinase C isozymes β 1 and β 2 were absent in nonpregnant myometrium but were induced during pregnancy. Labor at term did not alter protein kinase C isozyme expression.

Original languageEnglish
Pages (from-to)1525-1531
Number of pages7
JournalAmerican Journal of Obstetrics and Gynecology
Volume183
Issue number6
DOIs
StatePublished - 2000

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Myometrium
Protein Kinase C
Isoenzymes
Primary Cell Culture
Hysterectomy
Cultured Cells
Western Blotting
Monoclonal Antibodies
Pregnancy

Keywords

  • Human
  • Isozymes
  • Myometrium
  • Pregnancy
  • Protein kinase C
  • Uterus

ASJC Scopus subject areas

  • Medicine(all)
  • Obstetrics and Gynecology

Cite this

Expression of protein kinase C isozymes in nonpregnant and pregnant human myometrium. / Hurd, William W.; Fomin, Victor P.; Natarajan, Viswanathan; Brown, Haywood L.; Bigsby, Robert; Singh, Dawn M.

In: American Journal of Obstetrics and Gynecology, Vol. 183, No. 6, 2000, p. 1525-1531.

Research output: Contribution to journalArticle

Hurd, William W. ; Fomin, Victor P. ; Natarajan, Viswanathan ; Brown, Haywood L. ; Bigsby, Robert ; Singh, Dawn M. / Expression of protein kinase C isozymes in nonpregnant and pregnant human myometrium. In: American Journal of Obstetrics and Gynecology. 2000 ; Vol. 183, No. 6. pp. 1525-1531.
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