Expression of PTEN in mycosis fungoides and correlation with loss of heterozygosity

Terrence M. Katona, Bruce R. Smoller, Alden L. Webb, Eyas M. Hattab, Ahmed Khalil, Kim M. Hiatt

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

BACKGROUND:: Mycosis fungoides (MF) exhibits a variety of underlying molecular defects including aberrations involving the PTEN tumor suppressor gene. Specifically, loss of heterozygosity of PTEN has been previously demonstrated. We hypothesize that abnormalities of PTEN may result in altered immunohistochemical expression of its protein product. METHODS:: Thirty-six MF specimens were stained with monoclonal antibody against PTEN protein. The percentage of nuclei retaining PTEN expression and the staining intensity was recorded. RESULTS:: Average percentage of lymphoma cells retaining expression of the PTEN protein was 92% within patch-stage lesions, 81.4% in plaque-stage lesions, and 81.1% in tumor-stage lesions. Average intensity of staining for patch-stage lesions was 2.90, 2.50 for plaque lesions and 2.44 for tumor lesions. Cases lacking loss of heterozygozity at PTEN (n = 6) had an average expression of 81% and an average intensity of staining of 2.42. Whereas, cases with loss of heterozygozity at PTEN (n = 6) had an average expression of 75% of cells with an average staining intensity of 2.33. CONCLUSIONS:: The percentage of cells retaining PTEN and staining intensity decrease from patch- to plaque-stage lesions, whereas both parameters show mild diminution in tumor lesions compared with plaque lesions. PTEN expression in a small sample seems to correlate with previous demonstration of loss of heterozygosity at the molecular level. Although a trend for loss of PTEN expression exists with histologic progression of MF, the effect is modest and may not represent the pivotal defect in MF pathogenesis.

Original languageEnglish (US)
Pages (from-to)555-560
Number of pages6
JournalAmerican Journal of Dermatopathology
Volume35
Issue number5
DOIs
StatePublished - Jul 1 2013

Keywords

  • cutaneous lymphoma
  • immunohistochemistry
  • mycosis fungoides
  • plaque
  • tumor
  • tumor suppressor gene

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Dermatology

Fingerprint Dive into the research topics of 'Expression of PTEN in mycosis fungoides and correlation with loss of heterozygosity'. Together they form a unique fingerprint.

  • Cite this

    Katona, T. M., Smoller, B. R., Webb, A. L., Hattab, E. M., Khalil, A., & Hiatt, K. M. (2013). Expression of PTEN in mycosis fungoides and correlation with loss of heterozygosity. American Journal of Dermatopathology, 35(5), 555-560. https://doi.org/10.1097/DAD.0b013e318276cc68