Expression of pulmonary metallothionein genes in late gestational lambs

Bruce R. Pitt, Margaret Schwarz, A. Regina Steve, Arthur B. Atlas, Paul Davies, Shium Ming Kuo, John S. Lazo

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Metallothioneins (MT) are low molecular weight proteins that are important in providing protection against heavy metals such as cadmium. Other precise physiologic roles for this family of proteins are less clear, but fetal hepatic cell proliferation and differentiation may be regulated through changes in MT levels and attendant MT-mediated regulation of zinc levels. The role of MT in other developing tissue, most notably lung, is far less clear. Although expression of MT has been reported to be extremely low in early postnatal and mature lung, we hypothesized that MT has a more ubiquitous role in organ development and that pulmonary MT levels may be elevated during periods of rapid lung growth. Thus, we studied expression of MT in late-gestation fetal lambs. Sheep are particularly useful because alveolarization of lung parenchyma occurs before birth (by d 120 of a normal 147-d gestation). Immunoreactive MT was localized to bronchial epithelium of fetal, newborn, and mature sheep. The intensity of staining was greatest in the 130-d gestational age (saccular) lung, where positive reaction product was noted in the cytoplasm and nucleus of alveolar epithelial and interstitial cells. We next evaluated MT expression in developing lung tissue using Northern blot analysis and32P-cDNA probes against the 3’-untranslated regions of mRNA encoding each of four known functional sheep MT (sMT) isoforms. Expression of sMT-II, sMT-Ia, and sMT-Ib was restricted to the saccular stage (120-132 d gestational age), and sMT-Ic mRNA was not detected in pulmonary samples from any stage of development. In contrast, all mRNA isoforms were expressed in fetal and newborn liver and at levels considerably greater than lung. These data show that pulmonary MT gene expression is apparent in the saccular stage of lung development when considerable remodeling of lung parenchyma occurs. In addition, an organ- and age-specific expression of MT mRNA isoforms in developing sheep lung and liver was noted for the first time, suggesting that MT isoforms may play specific roles in organ development.

Original languageEnglish (US)
Pages (from-to)424-430
Number of pages7
JournalPediatric Research
Volume32
Issue number4
StatePublished - 1992
Externally publishedYes

Fingerprint

Metallothionein
Lung
Sheep
Genes
RNA Isoforms
Gestational Age
Protein Isoforms
Alveolar Epithelial Cells
Pregnancy
Messenger RNA
Liver
3' Untranslated Regions
Heavy Metals
Cadmium
Northern Blotting
Zinc
Cell Differentiation
Hepatocytes
Cytoplasm
Proteins

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health

Cite this

Pitt, B. R., Schwarz, M., Steve, A. R., Atlas, A. B., Davies, P., Kuo, S. M., & Lazo, J. S. (1992). Expression of pulmonary metallothionein genes in late gestational lambs. Pediatric Research, 32(4), 424-430.

Expression of pulmonary metallothionein genes in late gestational lambs. / Pitt, Bruce R.; Schwarz, Margaret; Steve, A. Regina; Atlas, Arthur B.; Davies, Paul; Kuo, Shium Ming; Lazo, John S.

In: Pediatric Research, Vol. 32, No. 4, 1992, p. 424-430.

Research output: Contribution to journalArticle

Pitt, BR, Schwarz, M, Steve, AR, Atlas, AB, Davies, P, Kuo, SM & Lazo, JS 1992, 'Expression of pulmonary metallothionein genes in late gestational lambs', Pediatric Research, vol. 32, no. 4, pp. 424-430.
Pitt BR, Schwarz M, Steve AR, Atlas AB, Davies P, Kuo SM et al. Expression of pulmonary metallothionein genes in late gestational lambs. Pediatric Research. 1992;32(4):424-430.
Pitt, Bruce R. ; Schwarz, Margaret ; Steve, A. Regina ; Atlas, Arthur B. ; Davies, Paul ; Kuo, Shium Ming ; Lazo, John S. / Expression of pulmonary metallothionein genes in late gestational lambs. In: Pediatric Research. 1992 ; Vol. 32, No. 4. pp. 424-430.
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AU - Lazo, John S.

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