Expression of receptor activator of nuclear factor κb ligand in ligature-induced periodontitis in osteoporotic and non-osteoporotic rats

E. Allam, A. Draz, A. Hassan, A. Neamat, M. Galal, L. Windsor

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Background and Objective: This study investigated the expression of a key mediator that regulates differentiation of osteoclasts, receptor activator of nuclear factor κB ligand (RANKL), in rats with or without osteoporosis and periodontitis, to provide a better understanding of the association between these two diseases. Material and Methods: Forty adult Albino rats were divided into four groups: (1) control group; (2) experimentally induced periodontitis group; (3) experimentally induced osteoporosis group; and (4) experimentally induced osteoporosis and periodontitis group. At the end of the experimental period, blood samples were obtained and animals were sacrificed. Serum alkaline phosphatase (ALP) activity levels were measured. Histological evaluation and immunohistochemical detection of RANKL in the periodontal ligament and bone tissues were performed. Results: There were significantly higher ALP levels in all of the experimental groups than in the control group. The pathology observed in the histological sections from group 4 was more severe than in either group 2 or group 3. The percentage of RANKL-immunoreactive cells in both the periodontal ligament and bone tissues in group 4 (16.8 ± 5.1 and 11.2 ± 5.2%, respectively) was significantly higher (p <0.001) than in the other groups. In the periodontal ligament, the percentage of RANKL-immunoreactive cells in group 2 (10.1 ± 1.9%) was significantly higher (p <0.001) than in group 3 (5.3 ± 2.7%) and the control group (4.12 ± 1.5%). Conclusion: The increased bone loss observed in group 4 compared with either group 2 or group 3 supports the existence of an additive pathological effect of the two disease conditions. This is consistent with the increased RANKL expression observed in group 4.

Original languageEnglish (US)
Pages (from-to)136-142
Number of pages7
JournalJournal of Periodontal Research
Volume45
Issue number1
DOIs
StatePublished - Feb 2010

Fingerprint

Periodontal Ligament
Periodontitis
Cytoplasmic and Nuclear Receptors
Osteoporosis
Ligation
Ligands
Bone and Bones
Control Groups
Alkaline Phosphatase
Osteoclasts
Pathology
Serum

Keywords

  • Immunohistochemistry
  • Osteoporosis
  • Periodontitis
  • Receptor activator of nuclear factor κB ligand

ASJC Scopus subject areas

  • Periodontics

Cite this

Expression of receptor activator of nuclear factor κb ligand in ligature-induced periodontitis in osteoporotic and non-osteoporotic rats. / Allam, E.; Draz, A.; Hassan, A.; Neamat, A.; Galal, M.; Windsor, L.

In: Journal of Periodontal Research, Vol. 45, No. 1, 02.2010, p. 136-142.

Research output: Contribution to journalArticle

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abstract = "Background and Objective: This study investigated the expression of a key mediator that regulates differentiation of osteoclasts, receptor activator of nuclear factor κB ligand (RANKL), in rats with or without osteoporosis and periodontitis, to provide a better understanding of the association between these two diseases. Material and Methods: Forty adult Albino rats were divided into four groups: (1) control group; (2) experimentally induced periodontitis group; (3) experimentally induced osteoporosis group; and (4) experimentally induced osteoporosis and periodontitis group. At the end of the experimental period, blood samples were obtained and animals were sacrificed. Serum alkaline phosphatase (ALP) activity levels were measured. Histological evaluation and immunohistochemical detection of RANKL in the periodontal ligament and bone tissues were performed. Results: There were significantly higher ALP levels in all of the experimental groups than in the control group. The pathology observed in the histological sections from group 4 was more severe than in either group 2 or group 3. The percentage of RANKL-immunoreactive cells in both the periodontal ligament and bone tissues in group 4 (16.8 ± 5.1 and 11.2 ± 5.2{\%}, respectively) was significantly higher (p <0.001) than in the other groups. In the periodontal ligament, the percentage of RANKL-immunoreactive cells in group 2 (10.1 ± 1.9{\%}) was significantly higher (p <0.001) than in group 3 (5.3 ± 2.7{\%}) and the control group (4.12 ± 1.5{\%}). Conclusion: The increased bone loss observed in group 4 compared with either group 2 or group 3 supports the existence of an additive pathological effect of the two disease conditions. This is consistent with the increased RANKL expression observed in group 4.",
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AB - Background and Objective: This study investigated the expression of a key mediator that regulates differentiation of osteoclasts, receptor activator of nuclear factor κB ligand (RANKL), in rats with or without osteoporosis and periodontitis, to provide a better understanding of the association between these two diseases. Material and Methods: Forty adult Albino rats were divided into four groups: (1) control group; (2) experimentally induced periodontitis group; (3) experimentally induced osteoporosis group; and (4) experimentally induced osteoporosis and periodontitis group. At the end of the experimental period, blood samples were obtained and animals were sacrificed. Serum alkaline phosphatase (ALP) activity levels were measured. Histological evaluation and immunohistochemical detection of RANKL in the periodontal ligament and bone tissues were performed. Results: There were significantly higher ALP levels in all of the experimental groups than in the control group. The pathology observed in the histological sections from group 4 was more severe than in either group 2 or group 3. The percentage of RANKL-immunoreactive cells in both the periodontal ligament and bone tissues in group 4 (16.8 ± 5.1 and 11.2 ± 5.2%, respectively) was significantly higher (p <0.001) than in the other groups. In the periodontal ligament, the percentage of RANKL-immunoreactive cells in group 2 (10.1 ± 1.9%) was significantly higher (p <0.001) than in group 3 (5.3 ± 2.7%) and the control group (4.12 ± 1.5%). Conclusion: The increased bone loss observed in group 4 compared with either group 2 or group 3 supports the existence of an additive pathological effect of the two disease conditions. This is consistent with the increased RANKL expression observed in group 4.

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