Expression of serotonin transporter protein in developing rat brain

Feng Zhou, Youssef Sari, Jing K. Zhang

Research output: Contribution to journalArticle

85 Citations (Scopus)

Abstract

Serotonin transporter (5-HTT), a transmembrane protein, has been shown in adult brain to be distributed not only on synaptic terminals but to a great extent on axons as well. Here we report the ontogeny of 5-HTT and its relationship with serotonin (5-HT) neurons using established 5-HTT and 5-HT antibodies. Both 5-HTT- and 5-HT-immunostaining (-im) appear in 5-HT neurons at embryonic day 12 (E12) in rostral raphe nuclei (RRN). Soon after appearing, 5-HTT-im is highly expressed on axons, similar to adult expression. However, in contrast to adult, 5-HTT-im also outlines the soma-dendrites. Rich 5-HTT-im appears along the entire length of projecting axons, extending to the growth tip. In the next 2 days, intensive 5-HTT-im axons from RRN travel a course preferentially in the floor plate and later, the medial forebrain bundle trajectory. A group of new 5-HT-im neurons and 5-HTT-im axons appear at E13 in caudal raphe nuclei. At E16-18, taking the exact trajectory course of 5-HT axons, 5-HTT-im axons reach ganglionic eminence, olfactory bulb, and cortex and disperse into many brain regions in E18-20. No 5-HTT-im cell bodies were seen in nigral, locus ceruleus, or hypothalamus. However, the transient expression of 5-HTT on non-serotonergic system was seen in cortical and striatal neuroepithelia at E12 and sensory thalamic pathways at P0-P10. Prominent 5-HTT-im fibers in thalamocortical bundles project from sensory thalamic nuclei through reticular nucleus, internal capsule bundle and form barrels in somatosensory cortices. No 5-HTT-im was seen in glia-like cells using currently available antibody. These observations indicate that 5-HTT is: (a) associated preferentially with 5-HT neurons in brainstem, (b) temporally co-expressed with 5-HT in 5-HT neurons, (c) expressed on axons prior to synaptical sites at target neurons, which strongly indicates a volumic (extrasynaptic) transmission, (d) expressed in non-5-HT neurons within a specific window, which may affect the development of the systems 'borrowing' the 5-HT. The early appearance of 5-HTT may also endow functionality as well as vulnerabilities of 5-HT, sensory thalamic, and cortical neurons to 5-HTT targeting drugs during pregnancy and after birth. Copyright (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)33-45
Number of pages13
JournalDevelopmental Brain Research
Volume119
Issue number1
DOIs
StatePublished - Jan 3 2000

Fingerprint

Serotonin Plasma Membrane Transport Proteins
Serotonin
Axons
Brain
Neurons
Proteins
Medial Forebrain Bundle
Internal Capsule
Corpus Striatum
Thalamic Nuclei
Raphe Nuclei
Somatosensory Cortex
Locus Coeruleus
Antibodies
Olfactory Bulb
Presynaptic Terminals
Carisoprodol
Substantia Nigra
Drug Delivery Systems
Dendrites

Keywords

  • Barrel formation
  • Development
  • Drug abuse
  • High-affinity uptake
  • Ontogeny
  • Serotonin, 5-HT
  • Somatosensory
  • Thalamocortical projection
  • Volume transmission

ASJC Scopus subject areas

  • Developmental Biology
  • Developmental Neuroscience

Cite this

Expression of serotonin transporter protein in developing rat brain. / Zhou, Feng; Sari, Youssef; Zhang, Jing K.

In: Developmental Brain Research, Vol. 119, No. 1, 03.01.2000, p. 33-45.

Research output: Contribution to journalArticle

Zhou, Feng ; Sari, Youssef ; Zhang, Jing K. / Expression of serotonin transporter protein in developing rat brain. In: Developmental Brain Research. 2000 ; Vol. 119, No. 1. pp. 33-45.
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