Expression of sphingosine 1-phosphate receptors in the rat dorsal root ganglia and defined single isolated sensory neurons

J. S. Kays, Chao Li, Grant Nicol

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Previously, we demonstrated that sphingosine 1-phosphate (S1P) increased the excitability of small-diameter sensory neurons, in part, through activation of S1P receptor 1 (S1PR1), suggesting that other S1PRs can modulate neuronal excitability. Therefore, studies were undertaken to establish the expression profiles of S1PRs in the intact dorsal root ganglion (DRG) and in defined single isolated sensory neurons. To determine mRNA expression of S1PRs in the DRG, SYBR green quantitative PCR (qPCR) was used. To determine the expression of S1PR mRNAs in single neurons of defined diameters, a preamplification protocol utilizing Taqman primer and probes was used to enhance the sensitivity of detection. The preamplification protocol also permitted detection of mRNA for two hallmark neuronal receptor/ion channels, TRPV1 and P2X3. Expression profiles of S1PR mRNA isolated from lung and brain were used as positive control tissues. In the intact DRG, the order of expression of S1PRs was S1PR3>>R1≈R2>R5≈R4. In the single neurons, the expression of S1PRs was quite variable with some neurons expressing all five subtypes, whereas some expressing only one subtype. In contrast to the DRG, S1PR1 was the highest expressing subtype in 10 of the 18 small-, medium-, and large-diameter sensory neurons. S1PR1 was the second highest expressor in ~50% of those remaining neurons. Overall, in the single neurons, the order of expression was S1PR1>>R3≈R5>R4>R2. The results obtained from the single defined neurons are consistent with our previous findings wherein S1PR1 plays a prominent but not exclusive role in the enhancement of neuronal excitability.

Original languageEnglish
Pages (from-to)889-901
Number of pages13
JournalPhysiological Genomics
Volume44
Issue number18
DOIs
StatePublished - Sep 1 2012

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Lysosphingolipid Receptors
Spinal Ganglia
Sensory Receptor Cells
Neurons
Messenger RNA
Ion Channels
Polymerase Chain Reaction
Lung
Brain

Keywords

  • Neuronal sensitization
  • Single cell qPCR
  • Taqman preamplification

ASJC Scopus subject areas

  • Physiology
  • Genetics

Cite this

Expression of sphingosine 1-phosphate receptors in the rat dorsal root ganglia and defined single isolated sensory neurons. / Kays, J. S.; Li, Chao; Nicol, Grant.

In: Physiological Genomics, Vol. 44, No. 18, 01.09.2012, p. 889-901.

Research output: Contribution to journalArticle

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