Expression of the CEA gene family members NCA-50/90 and NCA-160 (CD66) in childhood acute lymphoblastic leukemias (ALLs) and in cell lines of B-cell origin

Helmut Hanenberg, Margit Baumann, Iris Quentin, Gerhard Nagel, Hans Grosse-Wilde, Sabine Von Kleist, Ulrich Göbel, Stefan Burdach, Fritz Grunert

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The carcinoembryonic antigen (CEA) and the classical non-specific cross-reacting antigens (NCAs) belong to the CEA gene family which is part of the immunoglobulin super-family. In normal hematopoiesis, CEA gene family members (CGMs) have only been reported on cells of myeloid and monocytic origin. In the present study, we analyzed 62 childhood acute lymphoblastic leukemias (ALLs) and seven surface immunoglogulin positive (sig+) B-cell lines for the expression of the CEA family members CEA, NCA-50/90, NCA-95, NCA-160, CGM1 and CGM7. We demonstrated that members of the CEA family were present in 76% of childhood ALLs of B- and T-cell origin. In ALLs of B-cell origin, 82% of the samples expressed at least one CEA subgroup member: 38% NCA-50/90 (CD66c), 31% NCA-160 (CD66a), and 13% both. Six of seven B-cell lines solely expressed NCA-160. In seven ALL of T-cell origin, sole NCA-160 expression was present in 29% of the cases. CEA and CGM1 were not expressed in childhood ALLs or in the slg+ B-cell lines. In 15 ALLs and seven B-cell lines which could be analyzed for CGM7 expression, the antigen was not detected. NCA-95 was not expressed in 91% of the B-lineage ALLs, in T-lineage ALLs and in the B-cell lines. However, five B-lineage ALLs showed conflicting data on the binding patterns of two, on leukocytes specifically NCA-95 recognizing antibodies suggesting either expression of unknown forms of NCA-95 or NCA-50/90 or of a yet unknown member of the CEA family in these ALL cells. The expression of CEA subgroup members in childhood ALL cells might have prognostic impacts, as an inverse correlation exists between NCA expression on leukemic blasts and the risk factor white blood count at diagnosis.

Original languageEnglish (US)
Pages (from-to)2127-2133
Number of pages7
JournalLeukemia
Volume8
Issue number12
StatePublished - Dec 1994
Externally publishedYes

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Carcinoembryonic Antigen
Precursor Cell Lymphoblastic Leukemia-Lymphoma
B-Lymphocytes
Antigens
Cell Line
Genes
CD66 antigens
Biphenotypic Acute Leukemia
Precursor T-Cell Lymphoblastic Leukemia-Lymphoma
Hematopoiesis
Myeloid Cells
Immunoglobulins
Leukocytes

ASJC Scopus subject areas

  • Cancer Research
  • Hematology

Cite this

Hanenberg, H., Baumann, M., Quentin, I., Nagel, G., Grosse-Wilde, H., Von Kleist, S., ... Grunert, F. (1994). Expression of the CEA gene family members NCA-50/90 and NCA-160 (CD66) in childhood acute lymphoblastic leukemias (ALLs) and in cell lines of B-cell origin. Leukemia, 8(12), 2127-2133.

Expression of the CEA gene family members NCA-50/90 and NCA-160 (CD66) in childhood acute lymphoblastic leukemias (ALLs) and in cell lines of B-cell origin. / Hanenberg, Helmut; Baumann, Margit; Quentin, Iris; Nagel, Gerhard; Grosse-Wilde, Hans; Von Kleist, Sabine; Göbel, Ulrich; Burdach, Stefan; Grunert, Fritz.

In: Leukemia, Vol. 8, No. 12, 12.1994, p. 2127-2133.

Research output: Contribution to journalArticle

Hanenberg, H, Baumann, M, Quentin, I, Nagel, G, Grosse-Wilde, H, Von Kleist, S, Göbel, U, Burdach, S & Grunert, F 1994, 'Expression of the CEA gene family members NCA-50/90 and NCA-160 (CD66) in childhood acute lymphoblastic leukemias (ALLs) and in cell lines of B-cell origin', Leukemia, vol. 8, no. 12, pp. 2127-2133.
Hanenberg H, Baumann M, Quentin I, Nagel G, Grosse-Wilde H, Von Kleist S et al. Expression of the CEA gene family members NCA-50/90 and NCA-160 (CD66) in childhood acute lymphoblastic leukemias (ALLs) and in cell lines of B-cell origin. Leukemia. 1994 Dec;8(12):2127-2133.
Hanenberg, Helmut ; Baumann, Margit ; Quentin, Iris ; Nagel, Gerhard ; Grosse-Wilde, Hans ; Von Kleist, Sabine ; Göbel, Ulrich ; Burdach, Stefan ; Grunert, Fritz. / Expression of the CEA gene family members NCA-50/90 and NCA-160 (CD66) in childhood acute lymphoblastic leukemias (ALLs) and in cell lines of B-cell origin. In: Leukemia. 1994 ; Vol. 8, No. 12. pp. 2127-2133.
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title = "Expression of the CEA gene family members NCA-50/90 and NCA-160 (CD66) in childhood acute lymphoblastic leukemias (ALLs) and in cell lines of B-cell origin",
abstract = "The carcinoembryonic antigen (CEA) and the classical non-specific cross-reacting antigens (NCAs) belong to the CEA gene family which is part of the immunoglobulin super-family. In normal hematopoiesis, CEA gene family members (CGMs) have only been reported on cells of myeloid and monocytic origin. In the present study, we analyzed 62 childhood acute lymphoblastic leukemias (ALLs) and seven surface immunoglogulin positive (sig+) B-cell lines for the expression of the CEA family members CEA, NCA-50/90, NCA-95, NCA-160, CGM1 and CGM7. We demonstrated that members of the CEA family were present in 76{\%} of childhood ALLs of B- and T-cell origin. In ALLs of B-cell origin, 82{\%} of the samples expressed at least one CEA subgroup member: 38{\%} NCA-50/90 (CD66c), 31{\%} NCA-160 (CD66a), and 13{\%} both. Six of seven B-cell lines solely expressed NCA-160. In seven ALL of T-cell origin, sole NCA-160 expression was present in 29{\%} of the cases. CEA and CGM1 were not expressed in childhood ALLs or in the slg+ B-cell lines. In 15 ALLs and seven B-cell lines which could be analyzed for CGM7 expression, the antigen was not detected. NCA-95 was not expressed in 91{\%} of the B-lineage ALLs, in T-lineage ALLs and in the B-cell lines. However, five B-lineage ALLs showed conflicting data on the binding patterns of two, on leukocytes specifically NCA-95 recognizing antibodies suggesting either expression of unknown forms of NCA-95 or NCA-50/90 or of a yet unknown member of the CEA family in these ALL cells. The expression of CEA subgroup members in childhood ALL cells might have prognostic impacts, as an inverse correlation exists between NCA expression on leukemic blasts and the risk factor white blood count at diagnosis.",
author = "Helmut Hanenberg and Margit Baumann and Iris Quentin and Gerhard Nagel and Hans Grosse-Wilde and {Von Kleist}, Sabine and Ulrich G{\"o}bel and Stefan Burdach and Fritz Grunert",
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AU - Hanenberg, Helmut

AU - Baumann, Margit

AU - Quentin, Iris

AU - Nagel, Gerhard

AU - Grosse-Wilde, Hans

AU - Von Kleist, Sabine

AU - Göbel, Ulrich

AU - Burdach, Stefan

AU - Grunert, Fritz

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AB - The carcinoembryonic antigen (CEA) and the classical non-specific cross-reacting antigens (NCAs) belong to the CEA gene family which is part of the immunoglobulin super-family. In normal hematopoiesis, CEA gene family members (CGMs) have only been reported on cells of myeloid and monocytic origin. In the present study, we analyzed 62 childhood acute lymphoblastic leukemias (ALLs) and seven surface immunoglogulin positive (sig+) B-cell lines for the expression of the CEA family members CEA, NCA-50/90, NCA-95, NCA-160, CGM1 and CGM7. We demonstrated that members of the CEA family were present in 76% of childhood ALLs of B- and T-cell origin. In ALLs of B-cell origin, 82% of the samples expressed at least one CEA subgroup member: 38% NCA-50/90 (CD66c), 31% NCA-160 (CD66a), and 13% both. Six of seven B-cell lines solely expressed NCA-160. In seven ALL of T-cell origin, sole NCA-160 expression was present in 29% of the cases. CEA and CGM1 were not expressed in childhood ALLs or in the slg+ B-cell lines. In 15 ALLs and seven B-cell lines which could be analyzed for CGM7 expression, the antigen was not detected. NCA-95 was not expressed in 91% of the B-lineage ALLs, in T-lineage ALLs and in the B-cell lines. However, five B-lineage ALLs showed conflicting data on the binding patterns of two, on leukocytes specifically NCA-95 recognizing antibodies suggesting either expression of unknown forms of NCA-95 or NCA-50/90 or of a yet unknown member of the CEA family in these ALL cells. The expression of CEA subgroup members in childhood ALL cells might have prognostic impacts, as an inverse correlation exists between NCA expression on leukemic blasts and the risk factor white blood count at diagnosis.

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