Expression of the chemokine decoy receptor D6 mediates dendritic cell function and promotes corneal allograft rejection

Amir R. Hajrasouliha, Zahra Sadrai, Hyung K. Lee, Sunil K. Chauhan, Reza Dana

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Purpose: To identify the role of chemokine receptor 6 (D6) expression by dendritic cells (DCs) and its role in corneal transplant immunity. Methods: Flow cytometry analysis was used to assess the expression level of the D6 chemokine receptor in different leukocyte populations and DC maturation following lipopolysaccharides (LPS) stimulation of bone marrow-derived DCs isolated from wild-type (WT) or D6 -/- mice (C57BL/6 background). Mixed-lymphocyte reactions and delayed-type hypersensitivity assays were performed with bone marrow-derived DCs from WT or D6 -/- mice to evaluate T-cell al-loreactivity. Adoptive transfer experiments with T cells from WT or D6 -/- hosts with BALB/c corneal allografts were performed. Graft opacity was assessed over an 8-week period, and graft survival was plotted using Kaplan-Meier survival curves. Results: Expression of the D6 chemokine receptor was signifcantly higher in DCs compared to other leukocyte subpopu-lations, including neutrophils, lymphocytes, and monocytes/macrophages. LPS challenge of D6 -/- bone marrow-derived DCs elicited signifcantly lower levels of major histocompatibility complex II and costimulatory molecules (CD40, CD80, and CD86) compared to WT bone marrow-derived DCs, indicating the role of the D6 chemokine receptor in DC maturation. Further, DCs isolated from D6 -/- mice induced less T-cell proliferation (p≤0.001) and interferon-gamma production in T cells of draining lymph nodes compared to WT mice following corneal transplantation (p≤0.001). Moreover, adoptively transferred T cells from D6 -/- corneal transplanted mice to WT mice led to impaired graft rejection, compared to the hosts that received T cells from the WT transplanted mice. Conclusions: We demonstrated D6 chemokine receptor expression by DCs and identifed its critical function in multiple aspects of DC biology, including maturation and consequent elicitation of alloreactive T-cell responses that are responsible for corneal allograft rejection.

Original languageEnglish (US)
Pages (from-to)2517-2525
Number of pages9
JournalMolecular vision
Volume19
StatePublished - Dec 16 2013
Externally publishedYes

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Dendritic Cells
Allografts
T-Lymphocytes
Bone Marrow
Lipopolysaccharides
Leukocytes
chemokine receptor D6
Transplants
Mixed Lymphocyte Culture Test
Corneal Transplantation
Adoptive Transfer
Kaplan-Meier Estimate
Delayed Hypersensitivity
Graft Rejection
Graft Survival
Major Histocompatibility Complex
Inbred C57BL Mouse
Interferon-gamma
Cell Biology
Monocytes

ASJC Scopus subject areas

  • Ophthalmology

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Expression of the chemokine decoy receptor D6 mediates dendritic cell function and promotes corneal allograft rejection. / Hajrasouliha, Amir R.; Sadrai, Zahra; Lee, Hyung K.; Chauhan, Sunil K.; Dana, Reza.

In: Molecular vision, Vol. 19, 16.12.2013, p. 2517-2525.

Research output: Contribution to journalArticle

Hajrasouliha, Amir R. ; Sadrai, Zahra ; Lee, Hyung K. ; Chauhan, Sunil K. ; Dana, Reza. / Expression of the chemokine decoy receptor D6 mediates dendritic cell function and promotes corneal allograft rejection. In: Molecular vision. 2013 ; Vol. 19. pp. 2517-2525.
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abstract = "Purpose: To identify the role of chemokine receptor 6 (D6) expression by dendritic cells (DCs) and its role in corneal transplant immunity. Methods: Flow cytometry analysis was used to assess the expression level of the D6 chemokine receptor in different leukocyte populations and DC maturation following lipopolysaccharides (LPS) stimulation of bone marrow-derived DCs isolated from wild-type (WT) or D6 -/- mice (C57BL/6 background). Mixed-lymphocyte reactions and delayed-type hypersensitivity assays were performed with bone marrow-derived DCs from WT or D6 -/- mice to evaluate T-cell al-loreactivity. Adoptive transfer experiments with T cells from WT or D6 -/- hosts with BALB/c corneal allografts were performed. Graft opacity was assessed over an 8-week period, and graft survival was plotted using Kaplan-Meier survival curves. Results: Expression of the D6 chemokine receptor was signifcantly higher in DCs compared to other leukocyte subpopu-lations, including neutrophils, lymphocytes, and monocytes/macrophages. LPS challenge of D6 -/- bone marrow-derived DCs elicited signifcantly lower levels of major histocompatibility complex II and costimulatory molecules (CD40, CD80, and CD86) compared to WT bone marrow-derived DCs, indicating the role of the D6 chemokine receptor in DC maturation. Further, DCs isolated from D6 -/- mice induced less T-cell proliferation (p≤0.001) and interferon-gamma production in T cells of draining lymph nodes compared to WT mice following corneal transplantation (p≤0.001). Moreover, adoptively transferred T cells from D6 -/- corneal transplanted mice to WT mice led to impaired graft rejection, compared to the hosts that received T cells from the WT transplanted mice. Conclusions: We demonstrated D6 chemokine receptor expression by DCs and identifed its critical function in multiple aspects of DC biology, including maturation and consequent elicitation of alloreactive T-cell responses that are responsible for corneal allograft rejection.",
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AU - Chauhan, Sunil K.

AU - Dana, Reza

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N2 - Purpose: To identify the role of chemokine receptor 6 (D6) expression by dendritic cells (DCs) and its role in corneal transplant immunity. Methods: Flow cytometry analysis was used to assess the expression level of the D6 chemokine receptor in different leukocyte populations and DC maturation following lipopolysaccharides (LPS) stimulation of bone marrow-derived DCs isolated from wild-type (WT) or D6 -/- mice (C57BL/6 background). Mixed-lymphocyte reactions and delayed-type hypersensitivity assays were performed with bone marrow-derived DCs from WT or D6 -/- mice to evaluate T-cell al-loreactivity. Adoptive transfer experiments with T cells from WT or D6 -/- hosts with BALB/c corneal allografts were performed. Graft opacity was assessed over an 8-week period, and graft survival was plotted using Kaplan-Meier survival curves. Results: Expression of the D6 chemokine receptor was signifcantly higher in DCs compared to other leukocyte subpopu-lations, including neutrophils, lymphocytes, and monocytes/macrophages. LPS challenge of D6 -/- bone marrow-derived DCs elicited signifcantly lower levels of major histocompatibility complex II and costimulatory molecules (CD40, CD80, and CD86) compared to WT bone marrow-derived DCs, indicating the role of the D6 chemokine receptor in DC maturation. Further, DCs isolated from D6 -/- mice induced less T-cell proliferation (p≤0.001) and interferon-gamma production in T cells of draining lymph nodes compared to WT mice following corneal transplantation (p≤0.001). Moreover, adoptively transferred T cells from D6 -/- corneal transplanted mice to WT mice led to impaired graft rejection, compared to the hosts that received T cells from the WT transplanted mice. Conclusions: We demonstrated D6 chemokine receptor expression by DCs and identifed its critical function in multiple aspects of DC biology, including maturation and consequent elicitation of alloreactive T-cell responses that are responsible for corneal allograft rejection.

AB - Purpose: To identify the role of chemokine receptor 6 (D6) expression by dendritic cells (DCs) and its role in corneal transplant immunity. Methods: Flow cytometry analysis was used to assess the expression level of the D6 chemokine receptor in different leukocyte populations and DC maturation following lipopolysaccharides (LPS) stimulation of bone marrow-derived DCs isolated from wild-type (WT) or D6 -/- mice (C57BL/6 background). Mixed-lymphocyte reactions and delayed-type hypersensitivity assays were performed with bone marrow-derived DCs from WT or D6 -/- mice to evaluate T-cell al-loreactivity. Adoptive transfer experiments with T cells from WT or D6 -/- hosts with BALB/c corneal allografts were performed. Graft opacity was assessed over an 8-week period, and graft survival was plotted using Kaplan-Meier survival curves. Results: Expression of the D6 chemokine receptor was signifcantly higher in DCs compared to other leukocyte subpopu-lations, including neutrophils, lymphocytes, and monocytes/macrophages. LPS challenge of D6 -/- bone marrow-derived DCs elicited signifcantly lower levels of major histocompatibility complex II and costimulatory molecules (CD40, CD80, and CD86) compared to WT bone marrow-derived DCs, indicating the role of the D6 chemokine receptor in DC maturation. Further, DCs isolated from D6 -/- mice induced less T-cell proliferation (p≤0.001) and interferon-gamma production in T cells of draining lymph nodes compared to WT mice following corneal transplantation (p≤0.001). Moreover, adoptively transferred T cells from D6 -/- corneal transplanted mice to WT mice led to impaired graft rejection, compared to the hosts that received T cells from the WT transplanted mice. Conclusions: We demonstrated D6 chemokine receptor expression by DCs and identifed its critical function in multiple aspects of DC biology, including maturation and consequent elicitation of alloreactive T-cell responses that are responsible for corneal allograft rejection.

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