Expression of the type 1 and type 2 receptors for tumor necrosis factor after traumatic spinal cord injury in adult rats

Ping Yan, Naikui Liu, Gyeong Moon Kim, Jingming Xu, Jian Xu, Qun Li, Chung Y. Hsu, Xiao-Ming Xu

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

Posttraumatic inflammation has been implicated in secondary tissue damage after spinal cord injury (SCI). Tumor necrosis factor-α (TNF-α) is a key inflammatory mediator that is increasingly expressed after SCI. The effect of TNF-α is mediated through its receptors TNFR1 (p55) and TNFR2 (p75). However, whether these two receptors are expressed after SCI has not been demonstrated. In the present study, the temporo-spatial expression of TNFR1 and TNFR2 was examined in rats that had received a 10 g impact injury dropped at a height of 12.5 mm using the New York University impact device. In sham operates, no detectable TNFR1 or TNFR2 immunoreactivity (IR) was observed. In contused spinal cord, TNFR1 protein expression and immunoreactivity (IR) were detected as early as 15 min postinjury, reached its peak at 8 h, and declined markedly after 1 and 3 days postinjury. The temporal pattern of TNFR2 expression was similar to that of TNFR1 but its expression peaked at 4 h postinjury. During peak expression, TNFR1- and TNFR2-IR were most intense at the site of injury and decreased gradually from the injury epicenter. TNFR1- and TNFR2-positive cells included neurons, astrocytes, and oligodendrocytes. Methylprednisolone (MP), a synthetic glucocorticoid, partially inhibited the injury-induced expression of TNFR1 and TNFR2, an effect which could be reversed by RU486, an antagonist of glucocorticoid receptors. We suggest that the expression of TNFR1 and TNFR2 after SCI may contribute to posttraumatic inflammatory responses of TNF-α.

Original languageEnglish (US)
Pages (from-to)286-297
Number of pages12
JournalExperimental Neurology
Volume183
Issue number2
DOIs
StatePublished - Oct 1 2003
Externally publishedYes

Fingerprint

Receptors, Tumor Necrosis Factor, Type II
Receptors, Tumor Necrosis Factor, Type I
Spinal Cord Injuries
Tumor Necrosis Factor-alpha
Wounds and Injuries
Contusions
Oligodendroglia
Glucocorticoid Receptors
Methylprednisolone
Astrocytes
Glucocorticoids
Spinal Cord
Inflammation
Neurons
Equipment and Supplies

Keywords

  • Glucocorticoids
  • Inflammation
  • Rat
  • Spinal cord injury
  • TNF receptor
  • TNF-α

ASJC Scopus subject areas

  • Neurology
  • Neuroscience(all)

Cite this

Expression of the type 1 and type 2 receptors for tumor necrosis factor after traumatic spinal cord injury in adult rats. / Yan, Ping; Liu, Naikui; Kim, Gyeong Moon; Xu, Jingming; Xu, Jian; Li, Qun; Hsu, Chung Y.; Xu, Xiao-Ming.

In: Experimental Neurology, Vol. 183, No. 2, 01.10.2003, p. 286-297.

Research output: Contribution to journalArticle

Yan, Ping ; Liu, Naikui ; Kim, Gyeong Moon ; Xu, Jingming ; Xu, Jian ; Li, Qun ; Hsu, Chung Y. ; Xu, Xiao-Ming. / Expression of the type 1 and type 2 receptors for tumor necrosis factor after traumatic spinal cord injury in adult rats. In: Experimental Neurology. 2003 ; Vol. 183, No. 2. pp. 286-297.
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