Expression of the unconventional myosin Myo1c alters sodium transport in M1 collecting duct cells

Mark C. Wagner, Bonnie L. Blazer-Yost, Judy Boyd-White, Anjaiah Srirangam, Janice Pennington, Stacy Bennett

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

Epithelial cells rely on proper targeting of cellular components to perform their physiological function. This dynamic process utilizes the cytoskeleton and involves movement of vesicles to and from the plasma membrane, thus traversing the actin cortical cytoskeleton. Studies support both direct interaction of actin with channels and an indirect mechanism whereby actin may serve as a track in the final delivery of the channel to the plasma membrane. Actin-dependent processes are often mediated via a member of the myosin family of proteins. Myosin I family members have been implicated in multiple cellular events occurring at the plasma membrane. In these studies, we investigated the function of the unconventional myosin I Myo1c in the M1 mouse collecting duct cell line. Myo1c was observed to be concentrated at or near the plasma membrane, often in discrete membrane domains. To address the possible role of Myo1c in channel regulation, we expressed a truncated Myo1c, lacking ATP and actin domains, in M1 cells and compared electrophysiological responses to control M1 cells, M1 cells expressing the empty vector, and M1 cells expressing the full-length Myo1c construct. Interestingly, cells expressing the Myo1c constructs had modulated antidiuretic hormone (ADH)-stimulated short-circuit current and showed little inhibition of short-circuit current with amiloride addition. Evaluation of enhanced green fluorescent protein-Myo1c constructs supports the importance of the IQ region in targeting the Myo1c to its respective cellular domain. These data are consistent with Myo1c participating in the regulation of the Na+ channel after ADH stimulation.

Original languageEnglish (US)
Pages (from-to)C120-C129
JournalAmerican Journal of Physiology - Cell Physiology
Volume289
Issue number1 58-1
DOIs
StatePublished - Jul 1 2005

Keywords

  • Actin
  • Cytoskeleton
  • Ion channel
  • Kidney

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

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