Expression of trefoil peptides pS2 and human spasmolytic polypeptide in gastric metaplasia at the margin of duodenal ulcers

S. Khulusi, A. M. Hanby, J. M. Marrero, P. Patel, M. A. Mendall, Sunil Badve, R. Poulsom, G. Elia, N. A. Wright, T. C. Northfield

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Duodenal ulcers are associated with gastric metaplasia in the duodenum, both at the ulcer margin and at more distant sites in the duodenal bulb. pS2 and human spasmolytic polypeptide (hSP) are secretory peptides expressed in gastric epithelial cells and in gastric metaplasia. As these peptides may be important in ulcer healing, this study investigated the possibility that the expression of pS2 and hSP is increased in gastric metaplasia at the margin of duodenal ulcers. Duodenal bulb biopsy specimens from 12 duodenal ulcer patients were assessed. Sections were immunostained with monoclonal antibodies for pS2 and hSP. Cytoplasmic stain intensities were measured by an image analysis system and expressed as integrated optical density (IOD) units, In situ hybridisation for pS2 and hSP mRNA was carried out on parallel sections. Duodenal sections were also stained with diatase periodic acid Schiff/alcian blue to localise areas of gastric metaplasia. pS2 antigen staining in the duodenum was restricted to surface epithelial cells, and hSP to acinar and ductular components of Brunner's gland. mRNA localisation corresponded to immunostaining cells. In gastric metaplasia, pS2 expression was greater at the ulcer margin than away from the ulcer, as judged by the intensity of antibody staining (mean IOD units (SEM), 20.6 (3.3) v 9.5 (3.0); p <0.001). There was a trend towards greater hSP staining at the ulcer margin but this did not achieve statistical significance. These findings support the putative role of pS2 and possible hSP in mucosal healing and provide further evidence for an autocrine 'ulcer-gastric metaplasia-repair' loop involving these trefoil peptides.

Original languageEnglish (US)
Pages (from-to)205-209
Number of pages5
JournalGut
Volume37
Issue number2
StatePublished - 1995
Externally publishedYes

Fingerprint

Metaplasia
Duodenal Ulcer
Stomach
Ulcer
Staining and Labeling
Duodenum
Epithelial Cells
Brunner Glands
Alcian Blue
Messenger RNA
Peptides
Periodic Acid
Stomach Ulcer
spasmolytic polypeptide
Trefoil Factors
In Situ Hybridization
Coloring Agents
Monoclonal Antibodies
Biopsy
Antigens

Keywords

  • gastric metaplasia
  • trefoil peptides

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Khulusi, S., Hanby, A. M., Marrero, J. M., Patel, P., Mendall, M. A., Badve, S., ... Northfield, T. C. (1995). Expression of trefoil peptides pS2 and human spasmolytic polypeptide in gastric metaplasia at the margin of duodenal ulcers. Gut, 37(2), 205-209.

Expression of trefoil peptides pS2 and human spasmolytic polypeptide in gastric metaplasia at the margin of duodenal ulcers. / Khulusi, S.; Hanby, A. M.; Marrero, J. M.; Patel, P.; Mendall, M. A.; Badve, Sunil; Poulsom, R.; Elia, G.; Wright, N. A.; Northfield, T. C.

In: Gut, Vol. 37, No. 2, 1995, p. 205-209.

Research output: Contribution to journalArticle

Khulusi, S, Hanby, AM, Marrero, JM, Patel, P, Mendall, MA, Badve, S, Poulsom, R, Elia, G, Wright, NA & Northfield, TC 1995, 'Expression of trefoil peptides pS2 and human spasmolytic polypeptide in gastric metaplasia at the margin of duodenal ulcers', Gut, vol. 37, no. 2, pp. 205-209.
Khulusi, S. ; Hanby, A. M. ; Marrero, J. M. ; Patel, P. ; Mendall, M. A. ; Badve, Sunil ; Poulsom, R. ; Elia, G. ; Wright, N. A. ; Northfield, T. C. / Expression of trefoil peptides pS2 and human spasmolytic polypeptide in gastric metaplasia at the margin of duodenal ulcers. In: Gut. 1995 ; Vol. 37, No. 2. pp. 205-209.
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AU - Patel, P.

AU - Mendall, M. A.

AU - Badve, Sunil

AU - Poulsom, R.

AU - Elia, G.

AU - Wright, N. A.

AU - Northfield, T. C.

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N2 - Duodenal ulcers are associated with gastric metaplasia in the duodenum, both at the ulcer margin and at more distant sites in the duodenal bulb. pS2 and human spasmolytic polypeptide (hSP) are secretory peptides expressed in gastric epithelial cells and in gastric metaplasia. As these peptides may be important in ulcer healing, this study investigated the possibility that the expression of pS2 and hSP is increased in gastric metaplasia at the margin of duodenal ulcers. Duodenal bulb biopsy specimens from 12 duodenal ulcer patients were assessed. Sections were immunostained with monoclonal antibodies for pS2 and hSP. Cytoplasmic stain intensities were measured by an image analysis system and expressed as integrated optical density (IOD) units, In situ hybridisation for pS2 and hSP mRNA was carried out on parallel sections. Duodenal sections were also stained with diatase periodic acid Schiff/alcian blue to localise areas of gastric metaplasia. pS2 antigen staining in the duodenum was restricted to surface epithelial cells, and hSP to acinar and ductular components of Brunner's gland. mRNA localisation corresponded to immunostaining cells. In gastric metaplasia, pS2 expression was greater at the ulcer margin than away from the ulcer, as judged by the intensity of antibody staining (mean IOD units (SEM), 20.6 (3.3) v 9.5 (3.0); p <0.001). There was a trend towards greater hSP staining at the ulcer margin but this did not achieve statistical significance. These findings support the putative role of pS2 and possible hSP in mucosal healing and provide further evidence for an autocrine 'ulcer-gastric metaplasia-repair' loop involving these trefoil peptides.

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