Expression of Xenopus XlSALL4 during limb development and regeneration

Anton W. Neff, Michael King, Mark W. Harty, Trent Nguyen, John Calley, Rosamund C. Smith, Anthony Mescher

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The multi-C2H2 zinc-finger domain containing transcriptional regulators of the spalt (SAL) family plays important developmental regulatory roles. In a competitive subtractive hybridization screen of genes expressed in Xenopus laevis hindlimb regeneration blastemas, we identified a SAL family member that, by phylogenetic analysis, falls in the same clade as human SALL4 and have designated it as XlSALL4. Mutations of human SALL4 have been linked to Okihiro syndrome, which includes preaxial (anterior) limb defects. The expression pattern of XlSALL4 transcripts during normal forelimb and hindlimb development and during hindlimb regeneration at the regeneration-competent and regeneration-incompetent stages is temporally and regionally dynamic. We show for the first time that a SAL family member (XlSALL4) is expressed at the right place and time to play a role regulating both digit identity along the anterior/posterior axis and epimorphic limb regeneration.

Original languageEnglish
Pages (from-to)356-367
Number of pages12
JournalDevelopmental Dynamics
Volume233
Issue number2
DOIs
StatePublished - Jun 2005

Fingerprint

Xenopus
Regeneration
Extremities
Hindlimb
Duane Retraction Syndrome
Forelimb
Xenopus laevis
Mutation
Genes

Keywords

  • Development
  • Limb
  • Regeneration
  • SALL4
  • Xenopus laevis

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

Cite this

Expression of Xenopus XlSALL4 during limb development and regeneration. / Neff, Anton W.; King, Michael; Harty, Mark W.; Nguyen, Trent; Calley, John; Smith, Rosamund C.; Mescher, Anthony.

In: Developmental Dynamics, Vol. 233, No. 2, 06.2005, p. 356-367.

Research output: Contribution to journalArticle

Neff, Anton W. ; King, Michael ; Harty, Mark W. ; Nguyen, Trent ; Calley, John ; Smith, Rosamund C. ; Mescher, Anthony. / Expression of Xenopus XlSALL4 during limb development and regeneration. In: Developmental Dynamics. 2005 ; Vol. 233, No. 2. pp. 356-367.
@article{664e5657dace43ab96637698c87f7b74,
title = "Expression of Xenopus XlSALL4 during limb development and regeneration",
abstract = "The multi-C2H2 zinc-finger domain containing transcriptional regulators of the spalt (SAL) family plays important developmental regulatory roles. In a competitive subtractive hybridization screen of genes expressed in Xenopus laevis hindlimb regeneration blastemas, we identified a SAL family member that, by phylogenetic analysis, falls in the same clade as human SALL4 and have designated it as XlSALL4. Mutations of human SALL4 have been linked to Okihiro syndrome, which includes preaxial (anterior) limb defects. The expression pattern of XlSALL4 transcripts during normal forelimb and hindlimb development and during hindlimb regeneration at the regeneration-competent and regeneration-incompetent stages is temporally and regionally dynamic. We show for the first time that a SAL family member (XlSALL4) is expressed at the right place and time to play a role regulating both digit identity along the anterior/posterior axis and epimorphic limb regeneration.",
keywords = "Development, Limb, Regeneration, SALL4, Xenopus laevis",
author = "Neff, {Anton W.} and Michael King and Harty, {Mark W.} and Trent Nguyen and John Calley and Smith, {Rosamund C.} and Anthony Mescher",
year = "2005",
month = "6",
doi = "10.1002/dvdy.20363",
language = "English",
volume = "233",
pages = "356--367",
journal = "Developmental Dynamics",
issn = "1058-8388",
publisher = "Wiley-Liss Inc.",
number = "2",

}

TY - JOUR

T1 - Expression of Xenopus XlSALL4 during limb development and regeneration

AU - Neff, Anton W.

AU - King, Michael

AU - Harty, Mark W.

AU - Nguyen, Trent

AU - Calley, John

AU - Smith, Rosamund C.

AU - Mescher, Anthony

PY - 2005/6

Y1 - 2005/6

N2 - The multi-C2H2 zinc-finger domain containing transcriptional regulators of the spalt (SAL) family plays important developmental regulatory roles. In a competitive subtractive hybridization screen of genes expressed in Xenopus laevis hindlimb regeneration blastemas, we identified a SAL family member that, by phylogenetic analysis, falls in the same clade as human SALL4 and have designated it as XlSALL4. Mutations of human SALL4 have been linked to Okihiro syndrome, which includes preaxial (anterior) limb defects. The expression pattern of XlSALL4 transcripts during normal forelimb and hindlimb development and during hindlimb regeneration at the regeneration-competent and regeneration-incompetent stages is temporally and regionally dynamic. We show for the first time that a SAL family member (XlSALL4) is expressed at the right place and time to play a role regulating both digit identity along the anterior/posterior axis and epimorphic limb regeneration.

AB - The multi-C2H2 zinc-finger domain containing transcriptional regulators of the spalt (SAL) family plays important developmental regulatory roles. In a competitive subtractive hybridization screen of genes expressed in Xenopus laevis hindlimb regeneration blastemas, we identified a SAL family member that, by phylogenetic analysis, falls in the same clade as human SALL4 and have designated it as XlSALL4. Mutations of human SALL4 have been linked to Okihiro syndrome, which includes preaxial (anterior) limb defects. The expression pattern of XlSALL4 transcripts during normal forelimb and hindlimb development and during hindlimb regeneration at the regeneration-competent and regeneration-incompetent stages is temporally and regionally dynamic. We show for the first time that a SAL family member (XlSALL4) is expressed at the right place and time to play a role regulating both digit identity along the anterior/posterior axis and epimorphic limb regeneration.

KW - Development

KW - Limb

KW - Regeneration

KW - SALL4

KW - Xenopus laevis

UR - http://www.scopus.com/inward/record.url?scp=19544379537&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=19544379537&partnerID=8YFLogxK

U2 - 10.1002/dvdy.20363

DO - 10.1002/dvdy.20363

M3 - Article

VL - 233

SP - 356

EP - 367

JO - Developmental Dynamics

JF - Developmental Dynamics

SN - 1058-8388

IS - 2

ER -