Expression profiles frame the promoter specificity dilemma of the ETS family of transcription factors

Peter Hollenhorst, David A. Jones, Barbara J. Graves

Research output: Contribution to journalArticle

161 Citations (Scopus)

Abstract

Sequence-specific DNA binding proteins that function as transcription factors are frequently encoded by gene families. Such proteins display highly conserved DNA binding properties, yet are expected to retain promoter selectivity. In this report we investigate this problem using the ets gene family, a group of metazoan genes whose members regulate cell growth and differentiation and are mutated in human cancers. We, tested whether the level of mRNA can serve as a specificity determinant. The mRNA levels of the 27 paralogous human ets genes were measured in 23 tissues and cell lines. Real-time RT-PCR provided accurate measurement of absolute mRNA levels for each gene down to one copy per cell. Surprisingly, at least 16 paralogs were expressed in each cell sample and over half were expressed ubiquitously. Tissues and complementary cell lines showed similar expression patterns, indicating that tissue complexity was not a limitation. There was no unique, highly expressed gene for each cell type. Instead, one of only eight ets genes showed the highest expression in all samples. DNA binding studies illustrate both overlapping and unique specificities for ubiquitous ETS proteins. These findings establish the parameters of the promoter specificity dilemma within the ets family of transcription factors.

Original languageEnglish (US)
Pages (from-to)5693-5702
Number of pages10
JournalNucleic Acids Research
Volume32
Issue number18
DOIs
StatePublished - 2004
Externally publishedYes

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Transcription Factors
Genes
Messenger RNA
Proto-Oncogene Proteins c-ets
Cell Line
DNA
DNA-Binding Proteins
Real-Time Polymerase Chain Reaction
Cell Differentiation
Proteins
Growth
Neoplasms

ASJC Scopus subject areas

  • Genetics

Cite this

Expression profiles frame the promoter specificity dilemma of the ETS family of transcription factors. / Hollenhorst, Peter; Jones, David A.; Graves, Barbara J.

In: Nucleic Acids Research, Vol. 32, No. 18, 2004, p. 5693-5702.

Research output: Contribution to journalArticle

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