Expression profiling elucidates a molecular gene signature for pulmonary hypertension in sarcoidosis

Sunit Singla, Kamran Javaid, Taimur Abbasi, Michael S. Wade, Roberto Machado, Tong Zhou, Nancy Casanova, Joe G.N. Garcia, Wei Zhang, Shwu Fan Ma, Imre Noth, Nadera J. Sweiss

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Pulmonary hypertension (PH), when it complicates sarcoidosis, carries a poor prognosis, in part because it is difficult to detect early in patients with worsening respiratory symptoms. Pathogenesis of sarcoidosis occurs via incompletely characterized mechanisms that are distinct from the mechanisms of pulmonary vascular remodeling well known to occur in conjunction with other chronic lung diseases. To address the need for a biomarker to aid in early detection as well as the gap in knowledge regarding the mechanisms of PH in sarcoidosis, we used genome-wide peripheral blood gene expression analysis and identified an 18-gene signature capable of distinguishing sarcoidosis patients with PH (n = 8), sarcoidosis patients without PH (n = 17), and healthy controls (n = 45). The discriminative accuracy of this 18-gene signature was 100% in separating sarcoidosis patients with PH from those without it. If validated in a large replicate cohort, this signature could potentially be used as a diagnostic molecular biomarker for sarcoidosis-associated PH.

Original languageEnglish (US)
Pages (from-to)465-471
Number of pages7
JournalPulmonary Circulation
Volume6
Issue number4
DOIs
StatePublished - Dec 1 2016
Externally publishedYes

Fingerprint

Sarcoidosis
Pulmonary Hypertension
Genes
Biomarkers
Molecular Pathology
Lung Diseases
Chronic Disease
Genome
Gene Expression
Lung

Keywords

  • Gene signature
  • Peripheral blood mononuclear cells
  • Pulmonary hypertension
  • Sarcoidosis

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Cite this

Expression profiling elucidates a molecular gene signature for pulmonary hypertension in sarcoidosis. / Singla, Sunit; Javaid, Kamran; Abbasi, Taimur; Wade, Michael S.; Machado, Roberto; Zhou, Tong; Casanova, Nancy; Garcia, Joe G.N.; Zhang, Wei; Ma, Shwu Fan; Noth, Imre; Sweiss, Nadera J.

In: Pulmonary Circulation, Vol. 6, No. 4, 01.12.2016, p. 465-471.

Research output: Contribution to journalArticle

Singla, S, Javaid, K, Abbasi, T, Wade, MS, Machado, R, Zhou, T, Casanova, N, Garcia, JGN, Zhang, W, Ma, SF, Noth, I & Sweiss, NJ 2016, 'Expression profiling elucidates a molecular gene signature for pulmonary hypertension in sarcoidosis', Pulmonary Circulation, vol. 6, no. 4, pp. 465-471. https://doi.org/10.1086/688316
Singla, Sunit ; Javaid, Kamran ; Abbasi, Taimur ; Wade, Michael S. ; Machado, Roberto ; Zhou, Tong ; Casanova, Nancy ; Garcia, Joe G.N. ; Zhang, Wei ; Ma, Shwu Fan ; Noth, Imre ; Sweiss, Nadera J. / Expression profiling elucidates a molecular gene signature for pulmonary hypertension in sarcoidosis. In: Pulmonary Circulation. 2016 ; Vol. 6, No. 4. pp. 465-471.
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