Expression, sub cellular localization and putative function of platelet- type 12-lipoxygenase in human prostate cancer cell lines of different metastatic potential

József Timár, Erzsébet Rásó, Balázs Döme, Li Li, David Grignon, Daotai Nie, Kenneth V. Honn, Wolfgang Hagmann

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70 Scopus citations

Abstract

The involvement of 12-lipoxygenase (12-LOX) expression and function in tumor metastasis has been demonstrated in several murine tumor cell lines. In addition, 12-LOX expression was detected in human prostatic tumors and correlated to the clinical stage of disease. Here we provide data that human prostate cancer cell lines express the platelet-type isoform of 12-LOX at both the mRNA and protein levels, and immunohistochemistry revealed 12-LOX expression in human prostate tumors. The enzyme was localized to the plasma membrane, cytoplasmic organelles and nucleus in non-metastatic cells (PC-3 nm) and to the cytoskeleton and nucleus in metastatic cells (DU-145). After orthotopic/intraprostatic injection of tumor cells into SCID mice, the metastatic prostate carcinoma cells (DU-145) expressed 12-LOX at a significantly higher level corn pared with the non-metastatic counterparts, PC-3nm. The functional involvement of 12-LOX in the metastatic process was demonstrated when DU- 145 cells were pretreated in vitro with the 12-LOX inhibitors N-benzyl-N-hydroxy-5-phenylpentamide (BHPP) or baicalein, the use of which significantly inhibited lung colonization. These data suggest a potential involvement of 12-LOX in the progression of human prostate cancer. (C) 2000 Wiley-Liss, Inc.

Original languageEnglish (US)
Pages (from-to)37-43
Number of pages7
JournalInternational Journal of Cancer
Volume87
Issue number1
DOIs
StatePublished - Jan 1 2000

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ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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