Expression, sub cellular localization and putative function of platelet- type 12-lipoxygenase in human prostate cancer cell lines of different metastatic potential

József Timár, Erzsébet Rásó, Balázs Döme, Li Li, David Grignon, Daotai Nie, Kenneth V. Honn, Wolfgang Hagmann

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

The involvement of 12-lipoxygenase (12-LOX) expression and function in tumor metastasis has been demonstrated in several murine tumor cell lines. In addition, 12-LOX expression was detected in human prostatic tumors and correlated to the clinical stage of disease. Here we provide data that human prostate cancer cell lines express the platelet-type isoform of 12-LOX at both the mRNA and protein levels, and immunohistochemistry revealed 12-LOX expression in human prostate tumors. The enzyme was localized to the plasma membrane, cytoplasmic organelles and nucleus in non-metastatic cells (PC-3 nm) and to the cytoskeleton and nucleus in metastatic cells (DU-145). After orthotopic/intraprostatic injection of tumor cells into SCID mice, the metastatic prostate carcinoma cells (DU-145) expressed 12-LOX at a significantly higher level corn pared with the non-metastatic counterparts, PC-3nm. The functional involvement of 12-LOX in the metastatic process was demonstrated when DU- 145 cells were pretreated in vitro with the 12-LOX inhibitors N-benzyl-N-hydroxy-5-phenylpentamide (BHPP) or baicalein, the use of which significantly inhibited lung colonization. These data suggest a potential involvement of 12-LOX in the progression of human prostate cancer. (C) 2000 Wiley-Liss, Inc.

Original languageEnglish (US)
Pages (from-to)37-43
Number of pages7
JournalInternational Journal of Cancer
Volume87
Issue number1
DOIs
StatePublished - 2000
Externally publishedYes

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Arachidonate 12-Lipoxygenase
Prostatic Neoplasms
Blood Platelets
Cell Line
Prostate
Neoplasms
Lipoxygenase Inhibitors
SCID Mice
Tumor Cell Line
Cytoskeleton
Organelles
Zea mays
Protein Isoforms
Immunohistochemistry
Cell Membrane
Neoplasm Metastasis
Carcinoma
Lung
Messenger RNA
Injections

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Expression, sub cellular localization and putative function of platelet- type 12-lipoxygenase in human prostate cancer cell lines of different metastatic potential. / Timár, József; Rásó, Erzsébet; Döme, Balázs; Li, Li; Grignon, David; Nie, Daotai; Honn, Kenneth V.; Hagmann, Wolfgang.

In: International Journal of Cancer, Vol. 87, No. 1, 2000, p. 37-43.

Research output: Contribution to journalArticle

Timár, József ; Rásó, Erzsébet ; Döme, Balázs ; Li, Li ; Grignon, David ; Nie, Daotai ; Honn, Kenneth V. ; Hagmann, Wolfgang. / Expression, sub cellular localization and putative function of platelet- type 12-lipoxygenase in human prostate cancer cell lines of different metastatic potential. In: International Journal of Cancer. 2000 ; Vol. 87, No. 1. pp. 37-43.
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