Extensive variation and rapid shift of the MG192 sequence in Mycoplasma genitalium strains from patients with chronic infection

Liang Ma, Miriam Mancuso, James A. Williams, Barbara Van Der Pol, J. Fortenberry, Qiuyao Jia, Leann Myers, David H. Martin

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Mycoplasma genitalium causes persistent urogenital tract infection in humans. Antigenic variation of the protein encoded by the MG192 gene has been proposed as one of the mechanisms for persistence. The aims of this study were to determine MG192 sequence variation in patients with chronic M. genitalium infection and to analyze the sequence structural features of the MG192 gene and its encoded protein. Urogenital specimens were obtained from 13 patients who were followed for 10 days to 14 months. The variable region of the MG192 gene was PCR amplified, subcloned into plasmids, and sequenced. Sequence analysis of 220 plasmid clones yielded 97 unique MG192 variant sequences. MG192 sequence shift was identified between sequential specimens from all but one patient. Despite great variation of the MG192 gene among and within clinical specimens from different patients, MG192 sequences were more related within M. genitalium specimens from an individual patient than between patients. The MG192 variable region consisted of 11 discrete subvariable regions with different degrees of variability. Analysis of the two most variable regions (V4 and V6) in five sequential specimens from one patient showed that sequence changes increased over time and that most sequences were present at only one time point, suggesting immune selection. Topology analysis of the deduced MG192 protein predicted a surface-exposed membrane protein. Extensive variation of the MG192 sequence may not only change the antigenicity of the protein to allow immune evasion but also alter the mobility and adhesion ability of the organism to adapt to diverse host microenvironments, thus facilitating persistent infection.

Original languageEnglish
Pages (from-to)1326-1334
Number of pages9
JournalInfection and Immunity
Volume82
Issue number3
DOIs
StatePublished - Mar 2014

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Mycoplasma genitalium
Infection
Genes
Sequence Analysis
Proteins
Plasmids
Mycoplasma Infections
Immune Evasion
Antigenic Variation
Membrane Proteins
Clone Cells
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Parasitology
  • Infectious Diseases

Cite this

Extensive variation and rapid shift of the MG192 sequence in Mycoplasma genitalium strains from patients with chronic infection. / Ma, Liang; Mancuso, Miriam; Williams, James A.; Van Der Pol, Barbara; Fortenberry, J.; Jia, Qiuyao; Myers, Leann; Martin, David H.

In: Infection and Immunity, Vol. 82, No. 3, 03.2014, p. 1326-1334.

Research output: Contribution to journalArticle

Ma, Liang ; Mancuso, Miriam ; Williams, James A. ; Van Der Pol, Barbara ; Fortenberry, J. ; Jia, Qiuyao ; Myers, Leann ; Martin, David H. / Extensive variation and rapid shift of the MG192 sequence in Mycoplasma genitalium strains from patients with chronic infection. In: Infection and Immunity. 2014 ; Vol. 82, No. 3. pp. 1326-1334.
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