Extracellular purines are biomarkers of neutrophilic airway inflammation

C. R. Esther, N. E. Alexis, M. L. Clas, E. R. Lazarowski, S. H. Donaldson, C. M. Pedrosa Ribeiro, C. G. Moore, Stephanie Davis, R. C. Boucher

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

Purinergic signalling regulates airway defence mechanisms, suggesting that extracellular purines could serve as airway inflammation biomarkers in cystic fibrosis (CF). The purines adenosine triphosphate (ATP), adenosine diphosphate (ADP), adenosine monophosphate (AMP) and adenosine were measured in sputum from 21 adults (spontaneously expectorated from seven CF patients, induced from 14 healthy controls) to assess normal values and CF-associated changes. Subsequently, purine levels were measured in bronchoalveolar lavage fluid (BALF) from 37 children (25 CF patients, 12 disease controls) and compared with neutrophil counts, presence of airway infection and lung function. To noninvasively assess airway purines, ATP levels were measured using luminometry in exhaled breath condensate (EBC) from 14 children with CF and 14 healthy controls, then 14 CF children during a pulmonary exacerbation. Both ATP and AMP were elevated in sputum and BALF from CF subjects compared with controls. In BALF, ATP and AMP levels were inversely related to lung function and strongly correlated with neutrophil counts. In EBC, ATP levels were increased in CF relative to controls and decreased after treatment of CF pulmonary exacerbation. The purines adenosine triphosphate and adenosine monophosphate are candidate biomarkers of neutrophilic airways inflammation. Measurement of purines in sputum or exhaled breath condensate may provide a relatively simple and noninvasive method to track this inflammation. Copyright

Original languageEnglish (US)
Pages (from-to)949-956
Number of pages8
JournalEuropean Respiratory Journal
Volume31
Issue number5
DOIs
StatePublished - May 2008
Externally publishedYes

Fingerprint

Purines
Cystic Fibrosis
Biomarkers
Inflammation
Bronchoalveolar Lavage Fluid
Adenosine Monophosphate
Sputum
Adenosine Triphosphate
Lung
Neutrophils
Reference Values

Keywords

  • Biomarker
  • Bronchoalveolar lavage fluid
  • Cystic fibrosis
  • Exhaled breath condensate
  • Inflammation
  • Purinergic signalling

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Medicine(all)

Cite this

Esther, C. R., Alexis, N. E., Clas, M. L., Lazarowski, E. R., Donaldson, S. H., Pedrosa Ribeiro, C. M., ... Boucher, R. C. (2008). Extracellular purines are biomarkers of neutrophilic airway inflammation. European Respiratory Journal, 31(5), 949-956. https://doi.org/10.1183/09031936.00089807

Extracellular purines are biomarkers of neutrophilic airway inflammation. / Esther, C. R.; Alexis, N. E.; Clas, M. L.; Lazarowski, E. R.; Donaldson, S. H.; Pedrosa Ribeiro, C. M.; Moore, C. G.; Davis, Stephanie; Boucher, R. C.

In: European Respiratory Journal, Vol. 31, No. 5, 05.2008, p. 949-956.

Research output: Contribution to journalArticle

Esther, CR, Alexis, NE, Clas, ML, Lazarowski, ER, Donaldson, SH, Pedrosa Ribeiro, CM, Moore, CG, Davis, S & Boucher, RC 2008, 'Extracellular purines are biomarkers of neutrophilic airway inflammation', European Respiratory Journal, vol. 31, no. 5, pp. 949-956. https://doi.org/10.1183/09031936.00089807
Esther CR, Alexis NE, Clas ML, Lazarowski ER, Donaldson SH, Pedrosa Ribeiro CM et al. Extracellular purines are biomarkers of neutrophilic airway inflammation. European Respiratory Journal. 2008 May;31(5):949-956. https://doi.org/10.1183/09031936.00089807
Esther, C. R. ; Alexis, N. E. ; Clas, M. L. ; Lazarowski, E. R. ; Donaldson, S. H. ; Pedrosa Ribeiro, C. M. ; Moore, C. G. ; Davis, Stephanie ; Boucher, R. C. / Extracellular purines are biomarkers of neutrophilic airway inflammation. In: European Respiratory Journal. 2008 ; Vol. 31, No. 5. pp. 949-956.
@article{25a7a15451fe44eebae2dda60f2d5497,
title = "Extracellular purines are biomarkers of neutrophilic airway inflammation",
abstract = "Purinergic signalling regulates airway defence mechanisms, suggesting that extracellular purines could serve as airway inflammation biomarkers in cystic fibrosis (CF). The purines adenosine triphosphate (ATP), adenosine diphosphate (ADP), adenosine monophosphate (AMP) and adenosine were measured in sputum from 21 adults (spontaneously expectorated from seven CF patients, induced from 14 healthy controls) to assess normal values and CF-associated changes. Subsequently, purine levels were measured in bronchoalveolar lavage fluid (BALF) from 37 children (25 CF patients, 12 disease controls) and compared with neutrophil counts, presence of airway infection and lung function. To noninvasively assess airway purines, ATP levels were measured using luminometry in exhaled breath condensate (EBC) from 14 children with CF and 14 healthy controls, then 14 CF children during a pulmonary exacerbation. Both ATP and AMP were elevated in sputum and BALF from CF subjects compared with controls. In BALF, ATP and AMP levels were inversely related to lung function and strongly correlated with neutrophil counts. In EBC, ATP levels were increased in CF relative to controls and decreased after treatment of CF pulmonary exacerbation. The purines adenosine triphosphate and adenosine monophosphate are candidate biomarkers of neutrophilic airways inflammation. Measurement of purines in sputum or exhaled breath condensate may provide a relatively simple and noninvasive method to track this inflammation. Copyright",
keywords = "Biomarker, Bronchoalveolar lavage fluid, Cystic fibrosis, Exhaled breath condensate, Inflammation, Purinergic signalling",
author = "Esther, {C. R.} and Alexis, {N. E.} and Clas, {M. L.} and Lazarowski, {E. R.} and Donaldson, {S. H.} and {Pedrosa Ribeiro}, {C. M.} and Moore, {C. G.} and Stephanie Davis and Boucher, {R. C.}",
year = "2008",
month = "5",
doi = "10.1183/09031936.00089807",
language = "English (US)",
volume = "31",
pages = "949--956",
journal = "European Respiratory Journal",
issn = "0903-1936",
publisher = "European Respiratory Society",
number = "5",

}

TY - JOUR

T1 - Extracellular purines are biomarkers of neutrophilic airway inflammation

AU - Esther, C. R.

AU - Alexis, N. E.

AU - Clas, M. L.

AU - Lazarowski, E. R.

AU - Donaldson, S. H.

AU - Pedrosa Ribeiro, C. M.

AU - Moore, C. G.

AU - Davis, Stephanie

AU - Boucher, R. C.

PY - 2008/5

Y1 - 2008/5

N2 - Purinergic signalling regulates airway defence mechanisms, suggesting that extracellular purines could serve as airway inflammation biomarkers in cystic fibrosis (CF). The purines adenosine triphosphate (ATP), adenosine diphosphate (ADP), adenosine monophosphate (AMP) and adenosine were measured in sputum from 21 adults (spontaneously expectorated from seven CF patients, induced from 14 healthy controls) to assess normal values and CF-associated changes. Subsequently, purine levels were measured in bronchoalveolar lavage fluid (BALF) from 37 children (25 CF patients, 12 disease controls) and compared with neutrophil counts, presence of airway infection and lung function. To noninvasively assess airway purines, ATP levels were measured using luminometry in exhaled breath condensate (EBC) from 14 children with CF and 14 healthy controls, then 14 CF children during a pulmonary exacerbation. Both ATP and AMP were elevated in sputum and BALF from CF subjects compared with controls. In BALF, ATP and AMP levels were inversely related to lung function and strongly correlated with neutrophil counts. In EBC, ATP levels were increased in CF relative to controls and decreased after treatment of CF pulmonary exacerbation. The purines adenosine triphosphate and adenosine monophosphate are candidate biomarkers of neutrophilic airways inflammation. Measurement of purines in sputum or exhaled breath condensate may provide a relatively simple and noninvasive method to track this inflammation. Copyright

AB - Purinergic signalling regulates airway defence mechanisms, suggesting that extracellular purines could serve as airway inflammation biomarkers in cystic fibrosis (CF). The purines adenosine triphosphate (ATP), adenosine diphosphate (ADP), adenosine monophosphate (AMP) and adenosine were measured in sputum from 21 adults (spontaneously expectorated from seven CF patients, induced from 14 healthy controls) to assess normal values and CF-associated changes. Subsequently, purine levels were measured in bronchoalveolar lavage fluid (BALF) from 37 children (25 CF patients, 12 disease controls) and compared with neutrophil counts, presence of airway infection and lung function. To noninvasively assess airway purines, ATP levels were measured using luminometry in exhaled breath condensate (EBC) from 14 children with CF and 14 healthy controls, then 14 CF children during a pulmonary exacerbation. Both ATP and AMP were elevated in sputum and BALF from CF subjects compared with controls. In BALF, ATP and AMP levels were inversely related to lung function and strongly correlated with neutrophil counts. In EBC, ATP levels were increased in CF relative to controls and decreased after treatment of CF pulmonary exacerbation. The purines adenosine triphosphate and adenosine monophosphate are candidate biomarkers of neutrophilic airways inflammation. Measurement of purines in sputum or exhaled breath condensate may provide a relatively simple and noninvasive method to track this inflammation. Copyright

KW - Biomarker

KW - Bronchoalveolar lavage fluid

KW - Cystic fibrosis

KW - Exhaled breath condensate

KW - Inflammation

KW - Purinergic signalling

UR - http://www.scopus.com/inward/record.url?scp=47049124894&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=47049124894&partnerID=8YFLogxK

U2 - 10.1183/09031936.00089807

DO - 10.1183/09031936.00089807

M3 - Article

VL - 31

SP - 949

EP - 956

JO - European Respiratory Journal

JF - European Respiratory Journal

SN - 0903-1936

IS - 5

ER -