Extracellular signal-regulated kinases mediate the enhancing effects of inflammatory mediators on resurgent currents in dorsal root ganglion neurons

Bin Wu, Jeff S. McDermott, Jeffrey L. Krajewski, Kelly L. Knopp, Eric S. Nisenbaum, Theodore Cummins, Zhi Yong Tan

Research output: Contribution to journalArticle

Abstract

Previously we reported that a group of inflammatory mediators significantly enhanced resurgent currents in dorsal root ganglion neurons. To understand the underlying intracellular signaling mechanism, we investigated the effects of inhibition of extracellular signal-regulated kinases and protein kinase C on the enhancing effects of inflammatory mediators on resurgent currents in rat dorsal root ganglion neurons. We found that the extracellular signal-regulated kinases inhibitor U0126 completely prevented the enhancing effects of the inflammatory mediators on both Tetrodotoxin-sensitive and Tetrodotoxin-resistant resurgent currents in both small and medium dorsal root ganglion neurons. U0126 substantially reduced repetitive firing in small dorsal root ganglion neurons exposed to inflammatory mediators, consistent with prevention of resurgent current amplitude increases. The protein kinase C inhibitor Bisindolylmaleimide I also showed attenuating effects on resurgent currents, although to a lesser extent compared to extracellular signal-regulated kinases inhibition. These results indicate a critical role of extracellular signal-regulated kinases signaling in modulating resurgent currents and membrane excitability in dorsal root ganglion neurons treated with inflammatory mediators. It is also suggested that targeting extracellular signal-regulated kinases-resurgent currents might be a useful strategy to reduce inflammatory pain.

Original languageEnglish (US)
JournalMolecular Pain
Volume15
DOIs
StatePublished - Mar 1 2019

Fingerprint

Extracellular Signal-Regulated MAP Kinases
Spinal Ganglia
Neurons
Tetrodotoxin
Protein Kinase C
Protein C Inhibitor
Protein Kinase Inhibitors
Pain
Membranes
U 0126
Inhibition (Psychology)

Keywords

  • dorsal root ganglion
  • extracellular signal-regulated kinases
  • inflammatory mediators
  • protein kinase C
  • Resurgent currents
  • Tetrodotoxin-resistant
  • Tetrodotoxin-sensitive

ASJC Scopus subject areas

  • Molecular Medicine
  • Cellular and Molecular Neuroscience
  • Anesthesiology and Pain Medicine

Cite this

Extracellular signal-regulated kinases mediate the enhancing effects of inflammatory mediators on resurgent currents in dorsal root ganglion neurons. / Wu, Bin; McDermott, Jeff S.; Krajewski, Jeffrey L.; Knopp, Kelly L.; Nisenbaum, Eric S.; Cummins, Theodore; Tan, Zhi Yong.

In: Molecular Pain, Vol. 15, 01.03.2019.

Research output: Contribution to journalArticle

Wu, Bin ; McDermott, Jeff S. ; Krajewski, Jeffrey L. ; Knopp, Kelly L. ; Nisenbaum, Eric S. ; Cummins, Theodore ; Tan, Zhi Yong. / Extracellular signal-regulated kinases mediate the enhancing effects of inflammatory mediators on resurgent currents in dorsal root ganglion neurons. In: Molecular Pain. 2019 ; Vol. 15.
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