Extrinsic innervation of the rat kidney: A retrograde tracing study

V. H. Gattone, Carl Marfurt, S. Dallie

Research output: Chapter in Book/Report/Conference proceedingChapter

51 Citations (Scopus)

Abstract

To determine the exact modalities involved in the innervation of the kidney, the present study used a nerve-tracing method with horseradish peroxidase-wheat germ agglutinin (HRP-WGA) as the tracer. Multiple injections of HRP-WGA were made in each of the left kidneys of 12 rats while another four had the HRP-WGA either dripped onto their intact renal mesothelial surface of injected intravascularly. After retrograde transport of the tracer to neurons of origin (i.e., 72-h survival), the rats were briefly perfusion fixed, tissue was removed, and cryostat sections were cut. The free-floating sections were reacted by the tetramethylbenzidine technique. Retrogradely labeled neurons were found in the celiac, bilateral inferior vagal (nodosal), and ipsilateral dorsal root (90% in T12-L1 DRG) ganglia. More labeled neurons were present in the combined vagal ganglia than in the combined DRG within each animal. This labeling was specific compared with the controls (HRP-WGA uptake via intraperitoneal or vascular routes). The celiac ganglion had many labeled neurons; however, no labeled neurons were seen in the dorsal motor nucleus of the vagas, nucleus solitarius, nucleus ambiguus, or any other brain stem structure after renal injections of HRP-WGA. This study has determined that 1) the sympathetic nervous system (celiac ganglion) provides the only renal autonomic efferent (motor) innervation, and 2) the nodosal (inferior vagal) ganglia appear to provide more renal sensory innervation than do the dorsal root ganglia.

Original languageEnglish (US)
Title of host publicationAmerican Journal of Physiology - Renal Fluid and Electrolyte Physiology
Volume250
Edition2
StatePublished - 1986
Externally publishedYes

Fingerprint

Wheat Germ Agglutinins
Horseradish Peroxidase
Kidney
Neurons
Ganglia
Sympathetic Ganglia
Diagnosis-Related Groups
Medulla Oblongata
Injections
Solitary Nucleus
Spinal Nerve Roots
Sympathetic Nervous System
Spinal Ganglia
Abdomen
Brain Stem
Blood Vessels
Perfusion

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Gattone, V. H., Marfurt, C., & Dallie, S. (1986). Extrinsic innervation of the rat kidney: A retrograde tracing study. In American Journal of Physiology - Renal Fluid and Electrolyte Physiology (2 ed., Vol. 250)

Extrinsic innervation of the rat kidney : A retrograde tracing study. / Gattone, V. H.; Marfurt, Carl; Dallie, S.

American Journal of Physiology - Renal Fluid and Electrolyte Physiology. Vol. 250 2. ed. 1986.

Research output: Chapter in Book/Report/Conference proceedingChapter

Gattone, VH, Marfurt, C & Dallie, S 1986, Extrinsic innervation of the rat kidney: A retrograde tracing study. in American Journal of Physiology - Renal Fluid and Electrolyte Physiology. 2 edn, vol. 250.
Gattone VH, Marfurt C, Dallie S. Extrinsic innervation of the rat kidney: A retrograde tracing study. In American Journal of Physiology - Renal Fluid and Electrolyte Physiology. 2 ed. Vol. 250. 1986
Gattone, V. H. ; Marfurt, Carl ; Dallie, S. / Extrinsic innervation of the rat kidney : A retrograde tracing study. American Journal of Physiology - Renal Fluid and Electrolyte Physiology. Vol. 250 2. ed. 1986.
@inbook{4f1f025f27f343f7b9c6d229ec99297e,
title = "Extrinsic innervation of the rat kidney: A retrograde tracing study",
abstract = "To determine the exact modalities involved in the innervation of the kidney, the present study used a nerve-tracing method with horseradish peroxidase-wheat germ agglutinin (HRP-WGA) as the tracer. Multiple injections of HRP-WGA were made in each of the left kidneys of 12 rats while another four had the HRP-WGA either dripped onto their intact renal mesothelial surface of injected intravascularly. After retrograde transport of the tracer to neurons of origin (i.e., 72-h survival), the rats were briefly perfusion fixed, tissue was removed, and cryostat sections were cut. The free-floating sections were reacted by the tetramethylbenzidine technique. Retrogradely labeled neurons were found in the celiac, bilateral inferior vagal (nodosal), and ipsilateral dorsal root (90{\%} in T12-L1 DRG) ganglia. More labeled neurons were present in the combined vagal ganglia than in the combined DRG within each animal. This labeling was specific compared with the controls (HRP-WGA uptake via intraperitoneal or vascular routes). The celiac ganglion had many labeled neurons; however, no labeled neurons were seen in the dorsal motor nucleus of the vagas, nucleus solitarius, nucleus ambiguus, or any other brain stem structure after renal injections of HRP-WGA. This study has determined that 1) the sympathetic nervous system (celiac ganglion) provides the only renal autonomic efferent (motor) innervation, and 2) the nodosal (inferior vagal) ganglia appear to provide more renal sensory innervation than do the dorsal root ganglia.",
author = "Gattone, {V. H.} and Carl Marfurt and S. Dallie",
year = "1986",
language = "English (US)",
volume = "250",
booktitle = "American Journal of Physiology - Renal Fluid and Electrolyte Physiology",
edition = "2",

}

TY - CHAP

T1 - Extrinsic innervation of the rat kidney

T2 - A retrograde tracing study

AU - Gattone, V. H.

AU - Marfurt, Carl

AU - Dallie, S.

PY - 1986

Y1 - 1986

N2 - To determine the exact modalities involved in the innervation of the kidney, the present study used a nerve-tracing method with horseradish peroxidase-wheat germ agglutinin (HRP-WGA) as the tracer. Multiple injections of HRP-WGA were made in each of the left kidneys of 12 rats while another four had the HRP-WGA either dripped onto their intact renal mesothelial surface of injected intravascularly. After retrograde transport of the tracer to neurons of origin (i.e., 72-h survival), the rats were briefly perfusion fixed, tissue was removed, and cryostat sections were cut. The free-floating sections were reacted by the tetramethylbenzidine technique. Retrogradely labeled neurons were found in the celiac, bilateral inferior vagal (nodosal), and ipsilateral dorsal root (90% in T12-L1 DRG) ganglia. More labeled neurons were present in the combined vagal ganglia than in the combined DRG within each animal. This labeling was specific compared with the controls (HRP-WGA uptake via intraperitoneal or vascular routes). The celiac ganglion had many labeled neurons; however, no labeled neurons were seen in the dorsal motor nucleus of the vagas, nucleus solitarius, nucleus ambiguus, or any other brain stem structure after renal injections of HRP-WGA. This study has determined that 1) the sympathetic nervous system (celiac ganglion) provides the only renal autonomic efferent (motor) innervation, and 2) the nodosal (inferior vagal) ganglia appear to provide more renal sensory innervation than do the dorsal root ganglia.

AB - To determine the exact modalities involved in the innervation of the kidney, the present study used a nerve-tracing method with horseradish peroxidase-wheat germ agglutinin (HRP-WGA) as the tracer. Multiple injections of HRP-WGA were made in each of the left kidneys of 12 rats while another four had the HRP-WGA either dripped onto their intact renal mesothelial surface of injected intravascularly. After retrograde transport of the tracer to neurons of origin (i.e., 72-h survival), the rats were briefly perfusion fixed, tissue was removed, and cryostat sections were cut. The free-floating sections were reacted by the tetramethylbenzidine technique. Retrogradely labeled neurons were found in the celiac, bilateral inferior vagal (nodosal), and ipsilateral dorsal root (90% in T12-L1 DRG) ganglia. More labeled neurons were present in the combined vagal ganglia than in the combined DRG within each animal. This labeling was specific compared with the controls (HRP-WGA uptake via intraperitoneal or vascular routes). The celiac ganglion had many labeled neurons; however, no labeled neurons were seen in the dorsal motor nucleus of the vagas, nucleus solitarius, nucleus ambiguus, or any other brain stem structure after renal injections of HRP-WGA. This study has determined that 1) the sympathetic nervous system (celiac ganglion) provides the only renal autonomic efferent (motor) innervation, and 2) the nodosal (inferior vagal) ganglia appear to provide more renal sensory innervation than do the dorsal root ganglia.

UR - http://www.scopus.com/inward/record.url?scp=0022486403&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022486403&partnerID=8YFLogxK

M3 - Chapter

C2 - 3753828

AN - SCOPUS:0022486403

VL - 250

BT - American Journal of Physiology - Renal Fluid and Electrolyte Physiology

ER -