Facilitation of retrovirus-mediated gene transfer into hematopoietic stem and progenitor cells and peripheral blood T-lymphocytes utilizing recombinant fibronectin fragments

Karen E. Pollok, David A. Williams

Research output: Contribution to journalReview article

17 Scopus citations

Abstract

A transduction strategy has been developed, using fibronectin (FN)assisted retroviral-mediated gene transfer, based on the observation that hematopoietic stem and progenitor cells bind to specific adhesion domains of fibronectin, via the integrins, very late antigen-4 (VLA-4)α4β1 and very late antigen-5 (VLA-5)α5β1. Retrovirus-mediated transduction on a recombinant FN fragment, FN CH-296, containing binding sites for VLA-4 and VLA-5, separated by type III repeats 12 to 14, makes it possible to efficiently target hematopoietic stem and progenitor cells and T-lymphocytes due to colocalization of target cells and retrovirus particles. These gene therapy strategies are applicable to the potential treatment of a variety of acquired and inherited immune disorders.

Original languageEnglish (US)
Pages (from-to)595-604
Number of pages10
JournalCurrent Opinion in Molecular Therapeutics
Volume1
Issue number5
StatePublished - Nov 18 1999

Keywords

  • Colocalization
  • Fibronectin
  • Gene transfer
  • Retrovirus-mediated
  • VLA-4
  • VLA-5

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)
  • Pharmacology, Toxicology and Pharmaceutics(all)

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