Factors altering DNA synthesis in the cardiac myocyte of the adult newt, Notophthalmus viridescens

Mark Soonpaa, J. O. Oberpriller, J. C. Oberpriller

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

To better understand the mechanisms governing the proliferation of cardiac myocytes it is important to identify the factors controlling this phenomenon, and to characterize their actions. DNA synthesis was quantified in vitro in ventricular myocytes from the adult redspotted newt, Notophthalmus viridescens. Ventricles were enzymatically separated and plated onto laminin. Myocytes were fed modified L-15 medium with 10% fetal bovine serum, and were variously treated with transforming growth factor-beta, transforming growth factor-beta combined with platelet-derived growth factor, acidic fibroblast growth factor, basic fibroblast growth factor, 12-0-tetradecanoylphorbol-13-acetate, heparin, or conditioned medium from ventricular myocytes or non-myocytes (primarily endothelial cells). With their final feeding the cells were given 1 μCi/ml of tritiated thymidine, and 24 hours later were fixed and stained. Dishes were coated with photographic emulsion, exposed, and developed. The percent of cells with labeled nuclei was determined. Experimental media that significantly increased DNA synthesis included those containing acidic fibroblast growth factor (121% of control), basic fibroblast growth factor (119% of control), 12-0-tetradecanoylphorbol-13-acetate (233% of control) and conditioned medium from ventricular myocytes (230% of control) or non-myocytes (128% of control). Media significantly inhibiting DNA synthesis were those containing heparin (31% of control), transforming growth factor-beta (38% of control), non-myocyte conditioned medium and heparin (75% of control), or transforming growth factor-beta and platelet-derived growth factor (63% of control).

Original languageEnglish (US)
Pages (from-to)377-382
Number of pages6
JournalCell and Tissue Research
Volume275
Issue number2
DOIs
StatePublished - Feb 1994
Externally publishedYes

Fingerprint

Notophthalmus viridescens
Salamandridae
Fibroblast Growth Factors
Cardiac Myocytes
Transforming Growth Factor beta
Muscle Cells
Conditioned Culture Medium
Heparin
Fibroblast Growth Factor 1
DNA
Platelet-Derived Growth Factor
Tetradecanoylphorbol Acetate
Fibroblast Growth Factor 2
Laminin
Emulsions
Thymidine
Endothelial Cells
Acetates
Photographic emulsions
Serum

Keywords

  • Cardiac myocyte
  • Cell division
  • DNA synthesis
  • FGF
  • Notophthalmus viridescens (Urodela)
  • PDGF
  • TGF-β
  • TPa

ASJC Scopus subject areas

  • Anatomy
  • Clinical Biochemistry
  • Cell Biology

Cite this

Factors altering DNA synthesis in the cardiac myocyte of the adult newt, Notophthalmus viridescens. / Soonpaa, Mark; Oberpriller, J. O.; Oberpriller, J. C.

In: Cell and Tissue Research, Vol. 275, No. 2, 02.1994, p. 377-382.

Research output: Contribution to journalArticle

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