Failure of ethanol metabolites to alter gonadotropin secretion or luteinizing hormone synthesis in vitro

S. Uddin, L. Kirsteins, N. LaPaglia, N. V. Emanuele, A. M. Lawrence, M. R. Kelley, M. A. Emanuele

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The impact of ethanol on the male reproductive axis are multiple and varied, with both gonadal and control hypothalamic-pituitary pertubations being reported. There appears to be a discrepancy, however, between the in vivo and in vitro effects of ethanol on hypothalamic luteinizing hormones releasing hormone (LHRH) and the pituitary gonadotropins luteinizing hormone (LH) and follicle stimulating hormone (FSH). While in vivo data suggests a decrease in LHRH release after EtOH, in vitro studies find no effect on secretion. Similarily, in vivo acute EtOH profoundly diminishes LH synthesis and secretion, while in vitro impaired release with no alteration in the transcription of 3LH has been found. A potential exploration for these discrept results could be the in vivo metabolism of EtOH into acetaldehyde and acetate, or the subsequent formation of salsolinol, a product of acetate combining with dopamine. To test this possibility, a series of in vitro experiments were conducted exposing dispensed anterior pituitary cells from male rats to different doses of acetaldehyde, acetate or salsolinol for varying amounts of time for which gonadotropin secretion and 0LH mRNA levels were assessed. The results demonstrated no effect of either acetaldehyde or acetate on basal or LHRH stimulated LH release, FSH release or steady.

Original languageEnglish (US)
Pages (from-to)653-670
Number of pages18
JournalEndocrine Research
Volume21
Issue number3
DOIs
StatePublished - Jan 1995

Fingerprint

Luteinizing Hormone
Gonadotropins
Ethanol
Gonadotropin-Releasing Hormone
Acetaldehyde
Acetates
Hypothalamic Hormones
Follicle Stimulating Hormone
Endorphins
Pituitary Gonadotropins
Messenger RNA
Excitatory Amino Acids
Hyperprolactinemia
Substance P
Catecholamines
In Vitro Techniques
Dopamine

ASJC Scopus subject areas

  • Endocrinology

Cite this

Uddin, S., Kirsteins, L., LaPaglia, N., Emanuele, N. V., Lawrence, A. M., Kelley, M. R., & Emanuele, M. A. (1995). Failure of ethanol metabolites to alter gonadotropin secretion or luteinizing hormone synthesis in vitro. Endocrine Research, 21(3), 653-670. https://doi.org/10.1080/07435809509030481

Failure of ethanol metabolites to alter gonadotropin secretion or luteinizing hormone synthesis in vitro. / Uddin, S.; Kirsteins, L.; LaPaglia, N.; Emanuele, N. V.; Lawrence, A. M.; Kelley, M. R.; Emanuele, M. A.

In: Endocrine Research, Vol. 21, No. 3, 01.1995, p. 653-670.

Research output: Contribution to journalArticle

Uddin, S, Kirsteins, L, LaPaglia, N, Emanuele, NV, Lawrence, AM, Kelley, MR & Emanuele, MA 1995, 'Failure of ethanol metabolites to alter gonadotropin secretion or luteinizing hormone synthesis in vitro', Endocrine Research, vol. 21, no. 3, pp. 653-670. https://doi.org/10.1080/07435809509030481
Uddin, S. ; Kirsteins, L. ; LaPaglia, N. ; Emanuele, N. V. ; Lawrence, A. M. ; Kelley, M. R. ; Emanuele, M. A. / Failure of ethanol metabolites to alter gonadotropin secretion or luteinizing hormone synthesis in vitro. In: Endocrine Research. 1995 ; Vol. 21, No. 3. pp. 653-670.
@article{1e9b80bfb717427ab11addf12111a8d0,
title = "Failure of ethanol metabolites to alter gonadotropin secretion or luteinizing hormone synthesis in vitro",
abstract = "The impact of ethanol on the male reproductive axis are multiple and varied, with both gonadal and control hypothalamic-pituitary pertubations being reported. There appears to be a discrepancy, however, between the in vivo and in vitro effects of ethanol on hypothalamic luteinizing hormones releasing hormone (LHRH) and the pituitary gonadotropins luteinizing hormone (LH) and follicle stimulating hormone (FSH). While in vivo data suggests a decrease in LHRH release after EtOH, in vitro studies find no effect on secretion. Similarily, in vivo acute EtOH profoundly diminishes LH synthesis and secretion, while in vitro impaired release with no alteration in the transcription of 3LH has been found. A potential exploration for these discrept results could be the in vivo metabolism of EtOH into acetaldehyde and acetate, or the subsequent formation of salsolinol, a product of acetate combining with dopamine. To test this possibility, a series of in vitro experiments were conducted exposing dispensed anterior pituitary cells from male rats to different doses of acetaldehyde, acetate or salsolinol for varying amounts of time for which gonadotropin secretion and 0LH mRNA levels were assessed. The results demonstrated no effect of either acetaldehyde or acetate on basal or LHRH stimulated LH release, FSH release or steady.",
author = "S. Uddin and L. Kirsteins and N. LaPaglia and Emanuele, {N. V.} and Lawrence, {A. M.} and Kelley, {M. R.} and Emanuele, {M. A.}",
year = "1995",
month = "1",
doi = "10.1080/07435809509030481",
language = "English (US)",
volume = "21",
pages = "653--670",
journal = "Endocrine Research",
issn = "0743-5800",
publisher = "Informa Healthcare",
number = "3",

}

TY - JOUR

T1 - Failure of ethanol metabolites to alter gonadotropin secretion or luteinizing hormone synthesis in vitro

AU - Uddin, S.

AU - Kirsteins, L.

AU - LaPaglia, N.

AU - Emanuele, N. V.

AU - Lawrence, A. M.

AU - Kelley, M. R.

AU - Emanuele, M. A.

PY - 1995/1

Y1 - 1995/1

N2 - The impact of ethanol on the male reproductive axis are multiple and varied, with both gonadal and control hypothalamic-pituitary pertubations being reported. There appears to be a discrepancy, however, between the in vivo and in vitro effects of ethanol on hypothalamic luteinizing hormones releasing hormone (LHRH) and the pituitary gonadotropins luteinizing hormone (LH) and follicle stimulating hormone (FSH). While in vivo data suggests a decrease in LHRH release after EtOH, in vitro studies find no effect on secretion. Similarily, in vivo acute EtOH profoundly diminishes LH synthesis and secretion, while in vitro impaired release with no alteration in the transcription of 3LH has been found. A potential exploration for these discrept results could be the in vivo metabolism of EtOH into acetaldehyde and acetate, or the subsequent formation of salsolinol, a product of acetate combining with dopamine. To test this possibility, a series of in vitro experiments were conducted exposing dispensed anterior pituitary cells from male rats to different doses of acetaldehyde, acetate or salsolinol for varying amounts of time for which gonadotropin secretion and 0LH mRNA levels were assessed. The results demonstrated no effect of either acetaldehyde or acetate on basal or LHRH stimulated LH release, FSH release or steady.

AB - The impact of ethanol on the male reproductive axis are multiple and varied, with both gonadal and control hypothalamic-pituitary pertubations being reported. There appears to be a discrepancy, however, between the in vivo and in vitro effects of ethanol on hypothalamic luteinizing hormones releasing hormone (LHRH) and the pituitary gonadotropins luteinizing hormone (LH) and follicle stimulating hormone (FSH). While in vivo data suggests a decrease in LHRH release after EtOH, in vitro studies find no effect on secretion. Similarily, in vivo acute EtOH profoundly diminishes LH synthesis and secretion, while in vitro impaired release with no alteration in the transcription of 3LH has been found. A potential exploration for these discrept results could be the in vivo metabolism of EtOH into acetaldehyde and acetate, or the subsequent formation of salsolinol, a product of acetate combining with dopamine. To test this possibility, a series of in vitro experiments were conducted exposing dispensed anterior pituitary cells from male rats to different doses of acetaldehyde, acetate or salsolinol for varying amounts of time for which gonadotropin secretion and 0LH mRNA levels were assessed. The results demonstrated no effect of either acetaldehyde or acetate on basal or LHRH stimulated LH release, FSH release or steady.

UR - http://www.scopus.com/inward/record.url?scp=0029112867&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029112867&partnerID=8YFLogxK

U2 - 10.1080/07435809509030481

DO - 10.1080/07435809509030481

M3 - Article

C2 - 7588434

AN - SCOPUS:0029112867

VL - 21

SP - 653

EP - 670

JO - Endocrine Research

JF - Endocrine Research

SN - 0743-5800

IS - 3

ER -