Failure of ethanol to induce changes in gonadotropin gene expression in selectively bred ethanol-sensitive rats

Margaret M. Halloran, Mary Ann Emanuele, Laura Draski, John J. Tentler, Nicholas V. Emanuele, Mark Kelley

Research output: Contribution to journalArticle

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Abstract

The recent availability of genetically altered rat lines differing in sensitivity to ethanol (EtOH) has allowed deeper investigation into the mechanisms of EtOH-induced cellular toxicity in several systems. Since the male central reproductive axis has been demonstrated to be exquisitely sensitive to EtOH, studies were undertaken to determine if the gonadotropin suppression reported earlier could be duplicated in one of these selected rat lines. Castrated high alcohol sensitivity (HAS), low alcohol sensitivity (LAS) and control alcohol sensitivity (CAS) rats were given EtOH or saline acutely. Castrated non-selectively bred Sprague Dawley rats were treated similarily and used as an additional control. At sacrifice, serum and pituitary luteinizing hormone (LH) and follicle stimulating hormone (FSH) levels were obtained and the mRNA levels for both gonadotropins assessed. In the selectivity bred animal there was essentially no change in serum or pituitary LH or FSH levels between EtOH and saline treated animals. The mRNA levels for both LH and FSH similarly were unaffected by EtOH, in striking contrast to the non-selectively bred Sprague Dawley rats where serum LH, FSH and βLH mRNA levels are markedly suppressed after EtOH exposure. The selectively bred lines of rats genetically manipulated for high or low EtOH sensitivity, as well as their non-selected controls, appeared to have a hypothalamic-pituitary reproductive unit that is resistant to EtOH. This is in contrast to Sprague-Dawley rats, where suppression of this axis previously has been consistently demonstrated.

Original languageEnglish
Pages (from-to)317-329
Number of pages13
JournalEndocrine Research
Volume19
Issue number4
DOIs
StatePublished - 1993

Fingerprint

Luteinizing Hormone
Gonadotropins
Follicle Stimulating Hormone
Ethanol
Gene Expression
Sprague Dawley Rats
Pituitary Hormones
Alcohols
Messenger RNA
Serum
Hypothalamo-Hypophyseal System

ASJC Scopus subject areas

  • Endocrinology

Cite this

Failure of ethanol to induce changes in gonadotropin gene expression in selectively bred ethanol-sensitive rats. / Halloran, Margaret M.; Emanuele, Mary Ann; Draski, Laura; Tentler, John J.; Emanuele, Nicholas V.; Kelley, Mark.

In: Endocrine Research, Vol. 19, No. 4, 1993, p. 317-329.

Research output: Contribution to journalArticle

Halloran, Margaret M. ; Emanuele, Mary Ann ; Draski, Laura ; Tentler, John J. ; Emanuele, Nicholas V. ; Kelley, Mark. / Failure of ethanol to induce changes in gonadotropin gene expression in selectively bred ethanol-sensitive rats. In: Endocrine Research. 1993 ; Vol. 19, No. 4. pp. 317-329.
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