Fatty acid synthase gene overexpression and copy number gain in prostate adenocarcinoma

Uzma S. Shah, Rajiv Dhir, Susanne M. Gollin, Uma R. Chandran, Dale Lewis, Marie Acquafondata, Beth Pflug

Research output: Contribution to journalArticle

77 Citations (Scopus)

Abstract

Cancer cells frequently exhibit a significant increase in overexpression and activity of fatty acid synthase (FASN). Elevated FASN pathway activity also occurs in prostate cancer, the second leading cause of cancer-related death in men in the United States. Studies show that genes associated with an increase in protein expression, such as HER2/neu in breast cancer, are associated with an increase in gene copy number as well as an increase in transcription. In the present study, we evaluated whether FASN follows a similar paradigm in prostate cancer. To date, elevated FASN expression in prostate cancer has not been correlated with gene copy number alterations. Using immunohistochemistry and fluorescence in situ hybridization analysis in paraffin-embedded tissue microarrays, we observed gene copy gain in 24% of all prostate adenocarcinoma specimens examined with concurrent increased FASN protein expression. Immunohistochemistry alone showed 59% of prostate cancer specimens in the same tissue microarray with high FASN expression. Increased FASN gene was observed in 53% of all prostate tissues expressing elevated FASN protein levels and in 2 of 5 prostate tumor cell lines tested. These findings suggest that FASN gene copy number increases may be involved in the resultant increase in FASN protein expression observed in prostatic disease.

Original languageEnglish (US)
Pages (from-to)401-409
Number of pages9
JournalHuman Pathology
Volume37
Issue number4
DOIs
StatePublished - Apr 2006
Externally publishedYes

Fingerprint

Fatty Acid Synthases
Gene Dosage
Prostate
Adenocarcinoma
Prostatic Neoplasms
Proteins
Immunohistochemistry
Prostatic Diseases
Genes
Tumor Cell Line
Fluorescence In Situ Hybridization
Paraffin
Neoplasms
Breast Neoplasms

Keywords

  • Cytogenetics
  • Fatty acid synthase
  • FISH
  • Gene copy number
  • IHC
  • Prostate cancer

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Shah, U. S., Dhir, R., Gollin, S. M., Chandran, U. R., Lewis, D., Acquafondata, M., & Pflug, B. (2006). Fatty acid synthase gene overexpression and copy number gain in prostate adenocarcinoma. Human Pathology, 37(4), 401-409. https://doi.org/10.1016/j.humpath.2005.11.022

Fatty acid synthase gene overexpression and copy number gain in prostate adenocarcinoma. / Shah, Uzma S.; Dhir, Rajiv; Gollin, Susanne M.; Chandran, Uma R.; Lewis, Dale; Acquafondata, Marie; Pflug, Beth.

In: Human Pathology, Vol. 37, No. 4, 04.2006, p. 401-409.

Research output: Contribution to journalArticle

Shah, US, Dhir, R, Gollin, SM, Chandran, UR, Lewis, D, Acquafondata, M & Pflug, B 2006, 'Fatty acid synthase gene overexpression and copy number gain in prostate adenocarcinoma', Human Pathology, vol. 37, no. 4, pp. 401-409. https://doi.org/10.1016/j.humpath.2005.11.022
Shah US, Dhir R, Gollin SM, Chandran UR, Lewis D, Acquafondata M et al. Fatty acid synthase gene overexpression and copy number gain in prostate adenocarcinoma. Human Pathology. 2006 Apr;37(4):401-409. https://doi.org/10.1016/j.humpath.2005.11.022
Shah, Uzma S. ; Dhir, Rajiv ; Gollin, Susanne M. ; Chandran, Uma R. ; Lewis, Dale ; Acquafondata, Marie ; Pflug, Beth. / Fatty acid synthase gene overexpression and copy number gain in prostate adenocarcinoma. In: Human Pathology. 2006 ; Vol. 37, No. 4. pp. 401-409.
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