Fiber-optic immuno-biosensor for rapid and accurate detection of nerve growth factor in human blood

Liang Tang, Yong Mei Cha, Hongmei Li, Peng-Sheng Chen, Shien-Fong Lin

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Citations (Scopus)

Abstract

An accurate and rapid assay of cardiac nerve growth factor (NGF) levels in blood can provide physicians with critical information regarding myocardial injury and neural remodeling in cardiac tissues to identify patients at risk of impending heart attack, thereby enabling them to receive appropriate lifesaving treatment more quickly. Currently used assay methods, such as enzyme-linked immunosorbent assay (ELISA), are usually time-consuming (hours to days), expensive and technically complicated. In this paper, we described the development and clinical study of a rapid and sensitive method for detection and quantification of NGF in human blood plasma. This method utilizes a fiber-optic, immuno-biosensing system which performs a fluorophore-mediated sandwich immunoassay on the surface of an optical fiber. Physiological concentrations of NGF could be quantified in both buffer and human blood plasma samples within 5 minutes. The NGF concentrations determined by the fiber-optic sensor were comparable to those by the gold standard, ELISA. Preliminary study of NGF assay in cardiac patient plasma samples showed a great potential of the fiber-optic sensor as a rapid diagnostic and prognostic tool in clinical applications.

Original languageEnglish (US)
Title of host publicationAnnual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
Pages811-814
Number of pages4
DOIs
StatePublished - 2006
Externally publishedYes
Event28th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS'06 - New York, NY, United States
Duration: Aug 30 2006Sep 3 2006

Other

Other28th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS'06
CountryUnited States
CityNew York, NY
Period8/30/069/3/06

Fingerprint

Nerve Growth Factor
Biosensors
Fiber optics
Assays
Blood
Immunosorbents
Fiber optic sensors
Plasmas
Enzymes
Fluorophores
Optical fibers
Buffers
Intercellular Signaling Peptides and Proteins
Tissue

ASJC Scopus subject areas

  • Bioengineering

Cite this

Tang, L., Cha, Y. M., Li, H., Chen, P-S., & Lin, S-F. (2006). Fiber-optic immuno-biosensor for rapid and accurate detection of nerve growth factor in human blood. In Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings (pp. 811-814). [4029558] https://doi.org/10.1109/IEMBS.2006.260042

Fiber-optic immuno-biosensor for rapid and accurate detection of nerve growth factor in human blood. / Tang, Liang; Cha, Yong Mei; Li, Hongmei; Chen, Peng-Sheng; Lin, Shien-Fong.

Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. 2006. p. 811-814 4029558.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Tang, L, Cha, YM, Li, H, Chen, P-S & Lin, S-F 2006, Fiber-optic immuno-biosensor for rapid and accurate detection of nerve growth factor in human blood. in Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings., 4029558, pp. 811-814, 28th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS'06, New York, NY, United States, 8/30/06. https://doi.org/10.1109/IEMBS.2006.260042
Tang L, Cha YM, Li H, Chen P-S, Lin S-F. Fiber-optic immuno-biosensor for rapid and accurate detection of nerve growth factor in human blood. In Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. 2006. p. 811-814. 4029558 https://doi.org/10.1109/IEMBS.2006.260042
Tang, Liang ; Cha, Yong Mei ; Li, Hongmei ; Chen, Peng-Sheng ; Lin, Shien-Fong. / Fiber-optic immuno-biosensor for rapid and accurate detection of nerve growth factor in human blood. Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. 2006. pp. 811-814
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