Fiber type-specific determinants of Vmax for insulin-stimulated muscle glucose uptake in vivo

Hilary Ann Petersen, Patrick T. Fueger, Deanna P. Bracy, David H. Wasserman, Amy E. Halseth

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The aim of this study was to determine barriers limiting muscle glucose uptake (MGU) during increased glucose flux created by raising blood glucose in the presence of fixed insulin. The determinants of the maximal velocity (Vmax) of MGU in muscles of different fiber types were defined. Conscious rats were studied during a 4 mU·kg-1·min-1 insulin clamp with plasma glucose at 2.5, 5.5, and 8.5 mM. [U-14C] mannitol and 3-O-methyl-[3H]glucose ([3H]MG) were infused to steady-state levels (t = - 180 to 0 min). These isotope infusions were continued from 0 to 40 min with the addition of a 2-deoxy-[3H] glucose ([3H]DG) infusion. Muscles were excised at t = 40 min. Glucose metabolic index (Rg) was calculated from muscle-phosphorylated [3H] DG. [U-14C] mannitol was used to determine extracellular (EC) H2O. Glucose at the outer ([G]om) and inner ([G]im) sarcolemmal surfaces was determined by the ratio of [3H] MG in intracellular to EC H2O and muscle glucose. Rg was comparable at the two higher glucose concentrations, suggesting that rates of uptake near Vmax were reached. In summary, by defining the relationship of arterial glucose to [G]om and [G]im in the presence of fixed hyperinsulinemia, it is concluded that 1) Vmax for MGU is limited by extracellular and intracellular barriers in type I fibers, as the sarcolemma is freely permeable to glucose; 2) Vmax is limited in muscles with predominantly type IIb fibers by extracellular resistance and transport resistance; and 3) limits to Rg are determined by resistance at multiple steps and are better defined by distributed control rather than by a single rate-limiting step.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume284
Issue number3 47-3
StatePublished - Mar 1 2003
Externally publishedYes

Fingerprint

Muscle
Insulin
Glucose
Muscles
Fibers
Mannitol
Sarcolemma
Clamping devices
Hyperinsulinism
Isotopes
Blood Glucose
Rats
Fluxes
Plasmas

Keywords

  • Extracellular, intracellular water
  • Glucose analogs
  • Rat

ASJC Scopus subject areas

  • Physiology
  • Endocrinology
  • Biochemistry

Cite this

Petersen, H. A., Fueger, P. T., Bracy, D. P., Wasserman, D. H., & Halseth, A. E. (2003). Fiber type-specific determinants of Vmax for insulin-stimulated muscle glucose uptake in vivo. American Journal of Physiology - Endocrinology and Metabolism, 284(3 47-3).

Fiber type-specific determinants of Vmax for insulin-stimulated muscle glucose uptake in vivo. / Petersen, Hilary Ann; Fueger, Patrick T.; Bracy, Deanna P.; Wasserman, David H.; Halseth, Amy E.

In: American Journal of Physiology - Endocrinology and Metabolism, Vol. 284, No. 3 47-3, 01.03.2003.

Research output: Contribution to journalArticle

Petersen, Hilary Ann ; Fueger, Patrick T. ; Bracy, Deanna P. ; Wasserman, David H. ; Halseth, Amy E. / Fiber type-specific determinants of Vmax for insulin-stimulated muscle glucose uptake in vivo. In: American Journal of Physiology - Endocrinology and Metabolism. 2003 ; Vol. 284, No. 3 47-3.
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