Fibroblast growth factor protects nitric oxide-induced apoptosis in neuronal SHSY-5Y cells

A. Wagle, J. P. Singh

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Fibroblast growth factor (FGF) has been shown to protect tissue damage in animal models of cerebral and myocardial ischemia. The cellular and molecular mechanisms of FGF effects have not been fully defined. In the present study, we have investigated the effect of FGF homologs on nitric oxide (NO)-mediated neuronal cell death. Addition of NO donor S-nitroso-N-acetylpenicillamine (SNAP) to cultures of human neuroblastoma SHSY-5Y cells resulted in a concentration-dependent cell death. TdT-mediated dUTP-X nick end labeling and oligonucleosome assays confirmed that NO-mediated cell death occurred through the apoptotic pathway. In the presence of 150 μM SNAP, about 40% of the cells in culture underwent apoptosis. Treatment with FGF-2 resulted in greater than 80% reduction in NO-induced cell death. FGF addition to cell cultures also enhanced cell survival without affecting cell proliferation. FGF-2 effectively inhibited NO-mediated apoptosis even when added 6 h after treatment with SNAP. Examination of other homologs of FGF on NO-mediated cell death showed that in SHSY-5Y cells, FGF-2 and FGF-4, but not other FGF homologs, inhibited NO-mediated apoptosis. These results show that FGF-2 was a potent cell survival factor and protected SHSY-5Y cells from NO-mediated apoptosis. These effects were limited to FGF-2 and FGF-4 homologs.

Original languageEnglish (US)
Pages (from-to)889-895
Number of pages7
JournalJournal of Pharmacology and Experimental Therapeutics
Volume295
Issue number3
StatePublished - 2000
Externally publishedYes

Fingerprint

Fibroblast Growth Factors
Nitric Oxide
Fibroblast Growth Factor 2
Apoptosis
S-Nitroso-N-Acetylpenicillamine
Cell Death
Fibroblast Growth Factor 4
Cell Survival
Cell Culture Techniques
Nitric Oxide Donors
Brain Ischemia
Neuroblastoma
Myocardial Ischemia
Animal Models
Cell Proliferation

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology

Cite this

Fibroblast growth factor protects nitric oxide-induced apoptosis in neuronal SHSY-5Y cells. / Wagle, A.; Singh, J. P.

In: Journal of Pharmacology and Experimental Therapeutics, Vol. 295, No. 3, 2000, p. 889-895.

Research output: Contribution to journalArticle

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