Filamentous actin regulates insulin exocytosis through direct interaction with Syntaxin 4

Jenna L. Jewell, Wei Luo, Eunjin Oh, Zhanxiang Wang, Debbie C. Thurmond

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

Glucose-induced insulin exocytosis is coupled to associations between F-actin and SNARE proteins, although the nature and function of these interactions remains unknown. Toward this end we show here that both Syntaxin 1A and Syntaxin 4 associated with F-actin in MIN6 cells and that each interaction was rapidly and transiently diminished by stimulation of cells with D-glucose. Of the two isoforms, only Syntaxin 4 was capable of interacting directly with F-actin in an in vitro sedimentation assay, conferred by the N-terminal 39-112 residues of Syntaxin 4. The 39-112 fragment was capable of selective competitive inhibitory action, disrupting endogenous F-actin-Syntaxin 4 binding in MIN6 cells. Disruption of F-actin-Syntaxin 4 binding correlated with enhanced glucose-stimulated insulin secretion, mediated by increased granule accumulation at the plasma membrane and increased Syntaxin 4 accessibility under basal conditions. However, no increase in basal level Syntaxin4-VAMP2 association occurred with either latrunculin treatment or expression of the 39-112 fragment. Taken together, these data disclose a new underlying mechanism by which F-actin negatively regulates exocytosis via binding and blocking Syntaxin 4 accessibility, but they also reveal the existence of additional signals and/or steps required to trigger the subsequent docking and fusion steps of exocytosis.

Original languageEnglish
Pages (from-to)10716-10726
Number of pages11
JournalJournal of Biological Chemistry
Volume283
Issue number16
DOIs
StatePublished - Apr 18 2008

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Qa-SNARE Proteins
Exocytosis
Actins
Insulin
Glucose
Vesicle-Associated Membrane Protein 2
Syntaxin 1
SNARE Proteins
Cell membranes
Sedimentation
Cell Communication
Assays
Protein Isoforms
Fusion reactions
Cell Membrane
Association reactions

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Filamentous actin regulates insulin exocytosis through direct interaction with Syntaxin 4. / Jewell, Jenna L.; Luo, Wei; Oh, Eunjin; Wang, Zhanxiang; Thurmond, Debbie C.

In: Journal of Biological Chemistry, Vol. 283, No. 16, 18.04.2008, p. 10716-10726.

Research output: Contribution to journalArticle

Jewell, Jenna L. ; Luo, Wei ; Oh, Eunjin ; Wang, Zhanxiang ; Thurmond, Debbie C. / Filamentous actin regulates insulin exocytosis through direct interaction with Syntaxin 4. In: Journal of Biological Chemistry. 2008 ; Vol. 283, No. 16. pp. 10716-10726.
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