Fine needle aspiration of medullary carcinoma of the thyroid

Cytomorphology, immunocytochemistry and electron microscopy

B. T. Collins, Harvey Cramer, K. Tabatowski, S. Hearn, A. Raminhos, H. Lampe

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

OBJECTIVE: To analyze of medullary carcinoma of the thyroid (MCT) diagnosed by fine needle aspiration (FNA) utilizing cytomorphologic features and ancillary studies. STUDY DESIGN: Nine cases of MCT were collected, and the cytomorphologic findings were reviewed. Additionally, immunocytochemistry, immunoelectron microscopy and ultrastructural examination results were reviewed for selected cases. RESULTS: In five cases, loose groups predominated over single cells, whereas single cells predominated in three cases. One case showed only highly cohesive groups of cells. Most cells were round to oval, and every case had some degree of plasmacytoid morphology. Spindle-shaped cells were predominant in one case and were occasionally noted as a subpopulation in the other cases. Binucleation was noted in seven cases, and scattered, abnormally large nuclei were identified in five cases. The cytoplasm was moderate to abundant and delicate in all cases. Routine immunocytochemical staining for calcitonin and chromogranin was positive in three of four cases, and staining positive for the markers was detected by immunoelectron microscopy in two cases. In four cases, electron microscopy revealed neurosecretory granules. CONCLUSION: The cytomorphologic appearance of medullary thyroid carcinoma is highly distinctive, and the diagnosis can be corroborated by appropriate ancillary studies.

Original languageEnglish (US)
Pages (from-to)920-930
Number of pages11
JournalActa Cytologica
Volume39
Issue number5
StatePublished - 1995
Externally publishedYes

Fingerprint

Fine Needle Biopsy
Electron Microscopy
Immunohistochemistry
Immunoelectron Microscopy
Staining and Labeling
Chromogranins
Calcitonin
Cytoplasm
Medullary Thyroid cancer

Keywords

  • aspiration biopsy
  • carcinoma
  • electron microscopy
  • immunocytochemistry
  • thyroid neoplasms

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology
  • Histology

Cite this

Fine needle aspiration of medullary carcinoma of the thyroid : Cytomorphology, immunocytochemistry and electron microscopy. / Collins, B. T.; Cramer, Harvey; Tabatowski, K.; Hearn, S.; Raminhos, A.; Lampe, H.

In: Acta Cytologica, Vol. 39, No. 5, 1995, p. 920-930.

Research output: Contribution to journalArticle

Collins, BT, Cramer, H, Tabatowski, K, Hearn, S, Raminhos, A & Lampe, H 1995, 'Fine needle aspiration of medullary carcinoma of the thyroid: Cytomorphology, immunocytochemistry and electron microscopy', Acta Cytologica, vol. 39, no. 5, pp. 920-930.
Collins, B. T. ; Cramer, Harvey ; Tabatowski, K. ; Hearn, S. ; Raminhos, A. ; Lampe, H. / Fine needle aspiration of medullary carcinoma of the thyroid : Cytomorphology, immunocytochemistry and electron microscopy. In: Acta Cytologica. 1995 ; Vol. 39, No. 5. pp. 920-930.
@article{e2f972af1e8f42ff95b487d2dd1d9d8c,
title = "Fine needle aspiration of medullary carcinoma of the thyroid: Cytomorphology, immunocytochemistry and electron microscopy",
abstract = "OBJECTIVE: To analyze of medullary carcinoma of the thyroid (MCT) diagnosed by fine needle aspiration (FNA) utilizing cytomorphologic features and ancillary studies. STUDY DESIGN: Nine cases of MCT were collected, and the cytomorphologic findings were reviewed. Additionally, immunocytochemistry, immunoelectron microscopy and ultrastructural examination results were reviewed for selected cases. RESULTS: In five cases, loose groups predominated over single cells, whereas single cells predominated in three cases. One case showed only highly cohesive groups of cells. Most cells were round to oval, and every case had some degree of plasmacytoid morphology. Spindle-shaped cells were predominant in one case and were occasionally noted as a subpopulation in the other cases. Binucleation was noted in seven cases, and scattered, abnormally large nuclei were identified in five cases. The cytoplasm was moderate to abundant and delicate in all cases. Routine immunocytochemical staining for calcitonin and chromogranin was positive in three of four cases, and staining positive for the markers was detected by immunoelectron microscopy in two cases. In four cases, electron microscopy revealed neurosecretory granules. CONCLUSION: The cytomorphologic appearance of medullary thyroid carcinoma is highly distinctive, and the diagnosis can be corroborated by appropriate ancillary studies.",
keywords = "aspiration biopsy, carcinoma, electron microscopy, immunocytochemistry, thyroid neoplasms",
author = "Collins, {B. T.} and Harvey Cramer and K. Tabatowski and S. Hearn and A. Raminhos and H. Lampe",
year = "1995",
language = "English (US)",
volume = "39",
pages = "920--930",
journal = "Acta Cytologica",
issn = "0001-5547",
publisher = "Science Printers and Publishers Inc.",
number = "5",

}

TY - JOUR

T1 - Fine needle aspiration of medullary carcinoma of the thyroid

T2 - Cytomorphology, immunocytochemistry and electron microscopy

AU - Collins, B. T.

AU - Cramer, Harvey

AU - Tabatowski, K.

AU - Hearn, S.

AU - Raminhos, A.

AU - Lampe, H.

PY - 1995

Y1 - 1995

N2 - OBJECTIVE: To analyze of medullary carcinoma of the thyroid (MCT) diagnosed by fine needle aspiration (FNA) utilizing cytomorphologic features and ancillary studies. STUDY DESIGN: Nine cases of MCT were collected, and the cytomorphologic findings were reviewed. Additionally, immunocytochemistry, immunoelectron microscopy and ultrastructural examination results were reviewed for selected cases. RESULTS: In five cases, loose groups predominated over single cells, whereas single cells predominated in three cases. One case showed only highly cohesive groups of cells. Most cells were round to oval, and every case had some degree of plasmacytoid morphology. Spindle-shaped cells were predominant in one case and were occasionally noted as a subpopulation in the other cases. Binucleation was noted in seven cases, and scattered, abnormally large nuclei were identified in five cases. The cytoplasm was moderate to abundant and delicate in all cases. Routine immunocytochemical staining for calcitonin and chromogranin was positive in three of four cases, and staining positive for the markers was detected by immunoelectron microscopy in two cases. In four cases, electron microscopy revealed neurosecretory granules. CONCLUSION: The cytomorphologic appearance of medullary thyroid carcinoma is highly distinctive, and the diagnosis can be corroborated by appropriate ancillary studies.

AB - OBJECTIVE: To analyze of medullary carcinoma of the thyroid (MCT) diagnosed by fine needle aspiration (FNA) utilizing cytomorphologic features and ancillary studies. STUDY DESIGN: Nine cases of MCT were collected, and the cytomorphologic findings were reviewed. Additionally, immunocytochemistry, immunoelectron microscopy and ultrastructural examination results were reviewed for selected cases. RESULTS: In five cases, loose groups predominated over single cells, whereas single cells predominated in three cases. One case showed only highly cohesive groups of cells. Most cells were round to oval, and every case had some degree of plasmacytoid morphology. Spindle-shaped cells were predominant in one case and were occasionally noted as a subpopulation in the other cases. Binucleation was noted in seven cases, and scattered, abnormally large nuclei were identified in five cases. The cytoplasm was moderate to abundant and delicate in all cases. Routine immunocytochemical staining for calcitonin and chromogranin was positive in three of four cases, and staining positive for the markers was detected by immunoelectron microscopy in two cases. In four cases, electron microscopy revealed neurosecretory granules. CONCLUSION: The cytomorphologic appearance of medullary thyroid carcinoma is highly distinctive, and the diagnosis can be corroborated by appropriate ancillary studies.

KW - aspiration biopsy

KW - carcinoma

KW - electron microscopy

KW - immunocytochemistry

KW - thyroid neoplasms

UR - http://www.scopus.com/inward/record.url?scp=0029151544&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029151544&partnerID=8YFLogxK

M3 - Article

VL - 39

SP - 920

EP - 930

JO - Acta Cytologica

JF - Acta Cytologica

SN - 0001-5547

IS - 5

ER -