Flexibility in cell targeting by pseudotyping lentiviral vectors.

Daniela Bischof, Kenneth Cornetta

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

Lentiviral vectors have become an important research tool and have just entered into clinical trials. As wild-type lentiviruses engage specific receptors that have limited tropism, most investigators have replaced the endogenous envelope glycoprotein with an alternative envelope. Such pseudotyped vectors have the potential to infect a wide variety of cell types and species. Alternatively, selection of certain viral envelope glycoproteins may also facilitate cell targeting to enhance directed gene transfer. We describe the method for generating pseudotyped vector and provide information regarding available pseudotypes and their respective target tissues.

Original languageEnglish (US)
Pages (from-to)53-68
Number of pages16
JournalMethods in molecular biology (Clifton, N.J.)
Volume614
DOIs
StatePublished - 2010
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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