Flow cytometric analysis of DNA content and RAS P21 oncoprotein expression in ovarian neoplasms

Patricia K. Kotylo, Helen Michael, Naomi Fineberg, Gregory Sutton, Lawrence M. Roth

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Flow cytometric analysis of DNA content and ras p21 expression were studied in paraffin-embedded normal ovary(NO, n = 10), serous cyst-adenoma (SA, n = 11), serous tumors of low malignant potential (LMP, n = 13), and papillaryserous cystadenocarcinoma (SCa, n = 7). Tissue for DNA analysis was processed via a modified Hedley technique; a separate aliquot of the same sample was stained with a monoclonalantibody directed to oncoprotein ras p21 (clone Y13259). NO (10/10) and SA (11/11) demonstrated diploid DNA stemlines; 4/12 LMP and 5/7 SCa were aneuploid. S-phase fraction varied with significant differences (p < 0.001) for SA (mean = 4.4!%), LMP (mean = 9.0%), and SCa (mean = 12.7%). When all cases were evaluated, p21 expression was relatively lower in NO and SA (mean = 4.2%) in contrast to LMP and SCa (mean = 13.0% and 8.1%). Diploid cases were examined separately, and lesions with high p21 expression were associated with a diagnosis of LMP/SCa (p < 0.001), whereas diploid tissues with low p21 expression were associated with a nonmalignant diagnosis (NO/SA). This study suggests that aneuploidy or diploidy with high p21 expression (>10%) may be associated with LMP or frankly malignant ovarian tumors.

Original languageEnglish (US)
Pages (from-to)30-37
Number of pages8
JournalInternational Journal of Gynecological Pathology
Issue number1
StatePublished - Jan 1992



  • Cytometry
  • Dna ploidy
  • Ovarian neoplasms
  • p21

ASJC Scopus subject areas

  • Obstetrics and Gynecology
  • Pathology and Forensic Medicine

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