Flow cytometric analysis of hematopoietic development.

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

More than 30 yr ago, a collection of cells isolated from the bone marrow were first demonstrated to repopulate hematopoiesis in a radioablated animal. These cells self- renewed while producing all of the blood products and were named hematopoietic stem cells (HSCs). Since then, HSCs have been a tremendous boon to both basic science in understanding cell biology and as therapy in a cancer transplant setting. More recent work has shown that the HSCs, and possibly populations of cells residing in other tissues, have the properties of stem cells and the ability to repopulate nonrelated organs and tissues. This promiscuous repopulation has been termed plasticity and is the center of much research and even more debate. Our laboratory has recently proven that the HSCs meet the requirements of a stem cell by self-renewing and producing all of the blood lineages while concurrently demonstrating their ability to produce nonhematopoietic endothelial cells of blood vessels. This plasticity of the HSCs demonstrates hemangioblast activity, proven by both single cell and serial HSC transplants, and is the focus of this chapter.

Original languageEnglish
Pages (from-to)65-80
Number of pages16
JournalMethods in molecular medicine
Volume105
StatePublished - 2005

Fingerprint

Hematopoietic Stem Cells
Hemangioblasts
Stem Cells
Transplants
Hematopoiesis
Bone Marrow Cells
Blood Vessels
Cell Biology
Endothelial Cells
Research
Population
Neoplasms

Cite this

Flow cytometric analysis of hematopoietic development. / Srour, Edward; Yoder, Mervin.

In: Methods in molecular medicine, Vol. 105, 2005, p. 65-80.

Research output: Contribution to journalArticle

@article{553dbac598494468b41784067bae04cb,
title = "Flow cytometric analysis of hematopoietic development.",
abstract = "More than 30 yr ago, a collection of cells isolated from the bone marrow were first demonstrated to repopulate hematopoiesis in a radioablated animal. These cells self- renewed while producing all of the blood products and were named hematopoietic stem cells (HSCs). Since then, HSCs have been a tremendous boon to both basic science in understanding cell biology and as therapy in a cancer transplant setting. More recent work has shown that the HSCs, and possibly populations of cells residing in other tissues, have the properties of stem cells and the ability to repopulate nonrelated organs and tissues. This promiscuous repopulation has been termed plasticity and is the center of much research and even more debate. Our laboratory has recently proven that the HSCs meet the requirements of a stem cell by self-renewing and producing all of the blood lineages while concurrently demonstrating their ability to produce nonhematopoietic endothelial cells of blood vessels. This plasticity of the HSCs demonstrates hemangioblast activity, proven by both single cell and serial HSC transplants, and is the focus of this chapter.",
author = "Edward Srour and Mervin Yoder",
year = "2005",
language = "English",
volume = "105",
pages = "65--80",
journal = "Methods in molecular medicine",
issn = "1543-1894",
publisher = "Humana Press",

}

TY - JOUR

T1 - Flow cytometric analysis of hematopoietic development.

AU - Srour, Edward

AU - Yoder, Mervin

PY - 2005

Y1 - 2005

N2 - More than 30 yr ago, a collection of cells isolated from the bone marrow were first demonstrated to repopulate hematopoiesis in a radioablated animal. These cells self- renewed while producing all of the blood products and were named hematopoietic stem cells (HSCs). Since then, HSCs have been a tremendous boon to both basic science in understanding cell biology and as therapy in a cancer transplant setting. More recent work has shown that the HSCs, and possibly populations of cells residing in other tissues, have the properties of stem cells and the ability to repopulate nonrelated organs and tissues. This promiscuous repopulation has been termed plasticity and is the center of much research and even more debate. Our laboratory has recently proven that the HSCs meet the requirements of a stem cell by self-renewing and producing all of the blood lineages while concurrently demonstrating their ability to produce nonhematopoietic endothelial cells of blood vessels. This plasticity of the HSCs demonstrates hemangioblast activity, proven by both single cell and serial HSC transplants, and is the focus of this chapter.

AB - More than 30 yr ago, a collection of cells isolated from the bone marrow were first demonstrated to repopulate hematopoiesis in a radioablated animal. These cells self- renewed while producing all of the blood products and were named hematopoietic stem cells (HSCs). Since then, HSCs have been a tremendous boon to both basic science in understanding cell biology and as therapy in a cancer transplant setting. More recent work has shown that the HSCs, and possibly populations of cells residing in other tissues, have the properties of stem cells and the ability to repopulate nonrelated organs and tissues. This promiscuous repopulation has been termed plasticity and is the center of much research and even more debate. Our laboratory has recently proven that the HSCs meet the requirements of a stem cell by self-renewing and producing all of the blood lineages while concurrently demonstrating their ability to produce nonhematopoietic endothelial cells of blood vessels. This plasticity of the HSCs demonstrates hemangioblast activity, proven by both single cell and serial HSC transplants, and is the focus of this chapter.

UR - http://www.scopus.com/inward/record.url?scp=16644369882&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=16644369882&partnerID=8YFLogxK

M3 - Article

VL - 105

SP - 65

EP - 80

JO - Methods in molecular medicine

JF - Methods in molecular medicine

SN - 1543-1894

ER -