Flow cytometric immunophenotypic characterization of pediatric and adult minimally differentiated acute myeloid leukemia (AML-MO)

Patricia K. Kotylo, In Sook Seo, Franklin O. Smith, Nyla A. Heerema, Naomi S. Fineberg, Kathy Miller, Marianne E. Greene, Pauline Chou, Attilio Orazi

Research output: Contribution to journalArticlepeer-review

36 Scopus citations

Abstract

We reviewed the clinicopathologic and immunophenotypic profiles of 7 pediatric and 11 adult minimally differentiated acute myelogenous leukemias (AML-MO). We also compared and evaluated myeloperoxidase in leukemic blasts using standard cytochemical and polyclonal antibody immunohistochemical stains. No distinctive clinical findings were noted in either patient group; however, thrombocytopenia typically was more prominent in adults. Adult AML- MO also was associated with an immature myeloid profile (CD34+, terminal deoxynucleotidyl transferase positive, CD13+, and CD33+), in contrast with pediatric AML-MO, which usually lacked terminal deoxynucleotidyl transferase or CD34 but expressed bright CD33 with weak or negative CD13. Coexpression of the T-cell-associated antigen CD7 was observed in both groups. Antibody immunohistochemical stains were more sensitive than cytochemical stains for detection of myeloperoxidase activity and a useful adjunct for establishing a diagnosis of myeloid leukemia in paraffin-embedded marrow tissues.

Original languageEnglish (US)
Pages (from-to)193-200
Number of pages8
JournalAmerican journal of clinical pathology
Volume113
Issue number2
DOIs
StatePublished - 2000

Keywords

  • Adult
  • Immunophenotyping
  • Minimally differentiated acute myeloid leukemia
  • Pediatric

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Fingerprint Dive into the research topics of 'Flow cytometric immunophenotypic characterization of pediatric and adult minimally differentiated acute myeloid leukemia (AML-MO)'. Together they form a unique fingerprint.

Cite this