Flow cytometric immunophenotypic characterization of pediatric and adult minimally differentiated acute myeloid leukemia (AML-MO)

Patricia K. Kotylo, In Sook Seo, Franklin O. Smith, Nyla A. Heerema, Naomi S. Fineberg, Kathy Miller, Marianne E. Greene, Pauline Chou, Attilio Orazi

Research output: Contribution to journalArticle

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Abstract

We reviewed the clinicopathologic and immunophenotypic profiles of 7 pediatric and 11 adult minimally differentiated acute myelogenous leukemias (AML-MO). We also compared and evaluated myeloperoxidase in leukemic blasts using standard cytochemical and polyclonal antibody immunohistochemical stains. No distinctive clinical findings were noted in either patient group; however, thrombocytopenia typically was more prominent in adults. Adult AML- MO also was associated with an immature myeloid profile (CD34+, terminal deoxynucleotidyl transferase positive, CD13+, and CD33+), in contrast with pediatric AML-MO, which usually lacked terminal deoxynucleotidyl transferase or CD34 but expressed bright CD33 with weak or negative CD13. Coexpression of the T-cell-associated antigen CD7 was observed in both groups. Antibody immunohistochemical stains were more sensitive than cytochemical stains for detection of myeloperoxidase activity and a useful adjunct for establishing a diagnosis of myeloid leukemia in paraffin-embedded marrow tissues.

Original languageEnglish
Pages (from-to)193-200
Number of pages8
JournalAmerican Journal of Clinical Pathology
Volume113
Issue number2
StatePublished - 2000

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Acute Myeloid Leukemia
Coloring Agents
DNA Nucleotidylexotransferase
Pediatrics
Peroxidase
CD7 Antigens
Myeloid Leukemia
Antibodies
Thrombocytopenia
Paraffin
Bone Marrow
T-Lymphocytes

Keywords

  • Adult
  • Immunophenotyping
  • Minimally differentiated acute myeloid leukemia
  • Pediatric

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Flow cytometric immunophenotypic characterization of pediatric and adult minimally differentiated acute myeloid leukemia (AML-MO). / Kotylo, Patricia K.; Seo, In Sook; Smith, Franklin O.; Heerema, Nyla A.; Fineberg, Naomi S.; Miller, Kathy; Greene, Marianne E.; Chou, Pauline; Orazi, Attilio.

In: American Journal of Clinical Pathology, Vol. 113, No. 2, 2000, p. 193-200.

Research output: Contribution to journalArticle

Kotylo, PK, Seo, IS, Smith, FO, Heerema, NA, Fineberg, NS, Miller, K, Greene, ME, Chou, P & Orazi, A 2000, 'Flow cytometric immunophenotypic characterization of pediatric and adult minimally differentiated acute myeloid leukemia (AML-MO)', American Journal of Clinical Pathology, vol. 113, no. 2, pp. 193-200.
Kotylo, Patricia K. ; Seo, In Sook ; Smith, Franklin O. ; Heerema, Nyla A. ; Fineberg, Naomi S. ; Miller, Kathy ; Greene, Marianne E. ; Chou, Pauline ; Orazi, Attilio. / Flow cytometric immunophenotypic characterization of pediatric and adult minimally differentiated acute myeloid leukemia (AML-MO). In: American Journal of Clinical Pathology. 2000 ; Vol. 113, No. 2. pp. 193-200.
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AU - Greene, Marianne E.

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